【摘要】：目的探討微小RNA(miR)-424在宮頸上皮內(nèi)瘤變(CIN)和宮頸癌患者宮頸組織中的表達(dá)情況及其臨床意義。方法齊齊哈爾醫(yī)學(xué)院附屬第一醫(yī)院就診并最終獲得宮頸組織病理學(xué)診斷的40~69歲女性患者,收集宮頸組織;采用實(shí)時熒光定量PCR檢測不同病變程度宮頸組織中miR-424的相對表達(dá)水平并進(jìn)行比較。結(jié)果共收集129例不同程度病變患者的正常和病變宮頸組織樣本(CINⅠ組25例,CINⅡ組30例,CINⅢ組28例,宮頸癌組46例)。不同病變程度宮頸組織中miR-424的表達(dá)差異有統(tǒng)計學(xué)意義(F=47.009,P=0.000),且隨著病變程度的增加,miR-424的表達(dá)水平呈下降趨勢。miR-424高表達(dá)所占比例與宮頸組織病變程度相關(guān)(χ2=8.377,P=0.039),而與人乳頭瘤病毒(HPV)感染情況不相關(guān)。結(jié)論miR-424在不同病變宮頸的表達(dá)水平存在差異,隨著宮頸病變程度的加重,其表達(dá)水平呈降低趨勢,并且宮頸病變程度影響miR-424的表達(dá)水平。
[Abstract]:Objective to investigate the expression and clinical significance of RNA (miR)-424 in cervical tissues of patients with cervical intraepithelial neoplasia (CIN) and cervical carcinoma. Methods 4069 year old female patients with cervical histopathology were collected from the first affiliated Hospital of Qiqihar Medical College. Real-time fluorescence quantitative PCR was used to detect the relative expression of miR-424 in cervical tissues with different pathological degrees. Results the samples of normal and pathological cervical tissues were collected from 129 patients with different degrees of pathological changes (CIN 鈪，

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