【摘要】：目的檢測解整合素-金屬蛋白酶12在血清、絨毛及蛻膜組織中的表達(dá)含量，，胰島素樣生長因子II在絨毛組織內(nèi)的表達(dá)含量，旨在探討ADAM12可作為判斷早孕期胚胎發(fā)育水平的客觀指標(biāo)。 方法選擇早孕期女性（5~10周）49例，其中正常妊娠26例（占53.1％），稽留流產(chǎn)23例（占46.9％）；留取血清，采用酶聯(lián)免疫吸附實(shí)驗(yàn)法檢測血清ADAM12水平；收集人工流產(chǎn)負(fù)壓吸引術(shù)后絨毛組織及蛻膜組織，采用免疫組織化學(xué)染色方法，檢測其ADAM12蛋白表達(dá)水平。收集人工流產(chǎn)負(fù)壓吸引術(shù)后絨毛組織，采用免疫組織化學(xué)染色方法，檢測其IGF-II表達(dá)水平。 結(jié)果正常早孕女性血清ADAM12水平隨著孕周的增加而升高；在不同孕周，稽留流產(chǎn)組孕婦血清ADAM12含量均明顯低于同孕周的正常妊娠孕婦，且差異具有統(tǒng)計(jì)學(xué)意義（p＜0.05）；稽留流產(chǎn)組絨毛組織內(nèi)ADAM12表達(dá)量較少，且表達(dá)量低于正常妊娠組，差異具有統(tǒng)計(jì)學(xué)意義（p＜0.05）；在蛻膜組織內(nèi)情況相同，且差異具有統(tǒng)計(jì)學(xué)意義（p＜0.05）；稽留流產(chǎn)組絨毛組織內(nèi)IGF-II表達(dá)量較少，且表達(dá)量低于正常妊娠組，差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05）；正常早孕女性絨毛組織內(nèi)ADAM12與IGF-II表達(dá)量水平呈明顯正相關(guān)關(guān)系（r=0.369，p=0.016）。 結(jié)論正常早孕血清ADAM12水平隨著孕周的增加而升高；稽留流產(chǎn)組孕婦血清ADAM12水平明顯降低；稽留流產(chǎn)組孕婦絨毛組織及蛻膜組織內(nèi)ADAM12蛋白表達(dá)量均明顯降低；稽留流產(chǎn)組孕婦絨毛組織內(nèi)IGF-II表達(dá)量明顯降低；稽留流產(chǎn)組孕婦ADAM12含量的減少可以推測ADAM12可作為判斷早孕期胚胎發(fā)育水平的客觀指標(biāo)；ADAM12可以作為檢測指標(biāo)判斷早孕結(jié)局；正常早孕女性絨毛組織內(nèi)ADAM12與IGF-II表達(dá)量水平呈明顯正相關(guān)關(guān)系，可以推測二
[Abstract]:Objective to detect the expression of integrin-metalloproteinase-12 in serum, villi and decidua, and the expression of insulin-like growth factor II in chorionic villi. The aim of this study was to explore that ADAM12 could be used as an objective index to judge the level of embryonic development in early pregnancy. Methods A total of 49 women (510 weeks) with early pregnancy were selected, of whom 26 were normal pregnancy (53.1%), 23 were missed abortion (46.9%), and the serum was collected and detected by enzyme linked immunosorbent assay (Elisa). The chorionic villi and decidua tissues of induced abortion were collected and the expression of ADAM12 protein was detected by immunohistochemical staining. The expression of IGF-II was detected by immunohistochemical staining. Results the serum ADAM12 level of normal early pregnant women increased with the increase of gestational weeks, and the serum ADAM12 levels of women with missed abortion were significantly lower than those of normal pregnant women of the same gestational age (p < 0. 05). The expression of ADAM12 in chorionic villi of missed abortion group was lower than that in normal pregnancy group (p < 0. 05), the same in decidua tissue and the difference was statistically significant (p < 0. 05). The expression of IGF-II in chorionic villi of missed abortion group was lower than that in normal pregnancy group (P < 0. 05), and the expression level of ADAM12 and IGF-II in chorionic villi of women with normal early pregnancy was positively correlated (r 0. 369 p0. 016). Conclusion the serum ADAM12 level of normal early pregnancy increased with the increase of gestational age, the serum ADAM12 level of missed abortion group decreased significantly, and the expression of ADAM12 protein in villi and decidua tissues of missed abortion group decreased significantly. The expression of IGF-II in chorionic villi of missed abortion group was significantly decreased, and the decrease of ADAM12 content in missed abortion group could be speculated that ADAM12 could be used as an objective index to judge the level of embryonic development in early pregnancy. ADAM12 could be used as an index to judge the outcome of early pregnancy. There was a significant positive correlation between the expression of ADAM12 and IGF-II in the chorionic villi of normal early pregnant women.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R714.2

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