【摘要】：目的:探討噻可拉林協(xié)同順鉑促進宮頸癌c4-1、Hela細胞凋亡的機制。方法:用不同濃度噻可拉林和順鉑單獨或聯(lián)合作用于體外培養(yǎng)的c4-1、Hela細胞,MTT法檢測c4-1、Hela細胞生長抑制率,Hoechst 33342和AO-EB染色法檢測細胞凋亡,Western blot檢測細胞Bax、Bcl2、p53、p21蛋白的表達,流式細胞術(shù)檢測細胞增殖周期。結(jié)果:c4-1及Hela細胞經(jīng)不同濃度的噻可拉林和順鉑單獨或聯(lián)合作用后,細胞的體外增殖活力被明顯抑制,呈劑量依賴關(guān)系,且在聯(lián)合使用噻可拉林(10nmol/L):順鉑(4μmol/L)用量時抑制效果最明顯(P0.05);AO-EB染色法顯示,噻可拉林和順鉑聯(lián)合使用,細胞凋亡更明顯。Western blot則顯示,Bax、p53、p21表達明顯上調(diào),而Bcl2的表達則明顯下調(diào)。結(jié)論:噻可拉林能協(xié)同順鉑上調(diào)Bax、p53、p21的表達和下調(diào)Bcl2的表達,抑制宮頸癌c4-1、Hela細胞增殖同時促進細胞凋亡。
[Abstract]:Aim: to investigate the mechanism of tiacolarine combined with cisplatin in promoting apoptosis of cervical cancer cell line 4-1 Hela. Methods: different concentrations of thiacolamine and cisplatin were used to detect the growth inhibition rate of C4-1Hela cells alone or in combination with cisplatin in vitro. Hoechst 33342 and AO-EB staining were used to detect the expression of Bax,Bcl2,p53,p21 protein in C4-1Hela cells. Cell cycle was detected by flow cytometry. Results after treated with different concentrations of thiacolamine and cisplatin alone or in combination with different concentrations of thiacolamine and cisplatin, the proliferation activity of Hela cells was significantly inhibited in a dose-dependent manner. In combination with thiacolamine (10nmol/L) and cisplatin (4 渭 mol/L), the inhibitory effect was the most obvious (P0.05) in combination with cisplatin. Western blot showed that the expression of p53 p21 was up-regulated and the expression of Bcl2 was down-regulated in combination with thiacolamine and cisplatin. Conclusion: tiacolamine can increase the expression of Bax,p53,p21 and down-regulate the expression of Bcl2 in combination with cisplatin, inhibit the proliferation of C4-1Hela cells and promote apoptosis.
【作者單位】： 江漢大學(xué)附屬醫(yī)院婦產(chǎn)科;武漢市婦女兒童醫(yī)療保健中心婦產(chǎn)科;
【分類號】：R737.33
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本文編號：2233549