B7-H4和HK6在上皮性卵巢腫瘤中的表達(dá)及臨床意義
發(fā)布時(shí)間：2018-09-02 08:06
【摘要】：目的 通過檢測(cè)B7-H4蛋白和HK6蛋白在正常卵巢組織、卵巢上皮性良性腫瘤組織、交界性腫瘤組織以及惡性腫瘤組織中的表達(dá)水平，探討B(tài)7-H4蛋白和HK6蛋白在上皮性卵巢腫瘤的發(fā)生發(fā)展中的可能作用及其臨床意義。研究方法 本研究以70例卵巢上皮性腫瘤組織為研究對(duì)象，以10例正常卵巢組織為對(duì)照組，采用免疫組織化學(xué)染色SP法檢測(cè)B7-H4蛋白和HK6蛋白的表達(dá)，比較它們?cè)谡Ｂ殉步M織、卵巢上皮性良性、交界性及惡性腫瘤組織中的陽(yáng)性表達(dá)情況；并對(duì)二者在卵巢上皮性惡性腫瘤組織中的表達(dá)情況與患者的臨床病理參數(shù)之間的關(guān)系進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果 1、B7-H4蛋白在正常卵巢組織、卵巢上皮性良性、交界性及惡性腫瘤組織中的陽(yáng)性表達(dá)率分別為0.0%（0/10）、20.0%（3/15）、30.0%（3/10）、80.0%（36/45），在卵巢上皮性惡性腫瘤組的陽(yáng)性表達(dá)率明顯高于前三組，差異有統(tǒng)計(jì)學(xué)意義（χ2=31.192，P=0.000），而卵巢上皮性良性腫瘤組與交界性腫瘤組相比較，差異無(wú)統(tǒng)計(jì)學(xué)意義（χ2=0.009，P=0.924）。 2、B7-H4蛋白在卵巢上皮性惡性腫瘤組織中的表達(dá)： （1）≤50歲組的陽(yáng)性表達(dá)率為75.0%（6/8），＞50歲組的陽(yáng)性表達(dá)率為81.1%（30/37），兩者比較差異無(wú)統(tǒng)計(jì)學(xué)意義（χ2=0.000，P=1.000）； （2）漿液性癌、黏液性癌、子宮內(nèi)膜樣癌和透明細(xì)胞癌中的陽(yáng)性表達(dá)率分別為96.2%（25/26）、28.6%（2/7）、83.8%（5/6）和66.7%（4/6），在黏液性癌組的陽(yáng)性表達(dá)率為28.6%（2/7），明顯低于非粘液性癌組89.5%（34/38），差異有統(tǒng)計(jì)學(xué)意義（χ2=10.161，P=0.001），而非粘液性癌各組之間比較差異無(wú)統(tǒng)計(jì)學(xué)意義（χ2=4.052，P=0.132）； （3）Ⅰ~Ⅱ期的陽(yáng)性表達(dá)率為52.9%（9/17），明顯低于Ⅲ~Ⅳ期96.5%（27/28），差異具有統(tǒng)計(jì)學(xué)意義（χ2=9.932，P=0.002）； （4）高分化（G1）組陽(yáng)性表達(dá)率為37.5%（3/8），明顯低于中（G2）、低分化（G3）組89.2%（33/37），差異具有統(tǒng)計(jì)學(xué)意義（χ2=10.984，P=0.001）； （5）有淋巴結(jié)轉(zhuǎn)移組的陽(yáng)性表達(dá)率為94.7%（18/19），高于無(wú)淋巴結(jié)轉(zhuǎn)移組69.2%（18/26），差異具有統(tǒng)計(jì)學(xué)意義（χ2=4.464，P=0.035）； （6）有腹水組的陽(yáng)性表達(dá)率為85.7%（12/14），無(wú)腹水組的陽(yáng)性表達(dá)率為77.4%（24/31），兩者比較差異無(wú)統(tǒng)計(jì)學(xué)意義（χ2=0.058，P=0.809）。 3、HK6蛋白在正常卵巢組織、卵巢上皮性良性、交界性及惡性腫瘤組織中的陽(yáng)性表達(dá)率分別為10.0%（1/10）、13.3%（2/15）、50.0%（5/10）和60.0%（27/45）。HK6蛋白在卵巢上皮性良性腫瘤組中的陽(yáng)性表達(dá)率明顯低于交界性及惡性腫瘤組，差異有統(tǒng)計(jì)學(xué)意義（χ2=9.490，P=0.002），而交界性腫瘤組與惡性腫瘤組相比較，差異無(wú)統(tǒng)計(jì)學(xué)意義（χ2=0.051，P=0.822）。 4、HK6蛋白在卵巢惡性腫瘤組織中的表達(dá)： （1）≤50歲組的陽(yáng)性表達(dá)率為62.5%（5/8），＞50歲組的陽(yáng)性表達(dá)率為59.5%（22/37），兩者比較差異無(wú)統(tǒng)計(jì)學(xué)意義（χ2=0.000，P=1.000）； （2）漿液性癌、黏液性癌、子宮內(nèi)膜樣癌和透明細(xì)胞癌中的陽(yáng)性表達(dá)率分別為65.4%（17/26）、57.1%（4/7）、50.0%（3/6）和50.0%（3/6），各種不同組織學(xué)類型間相互比較，差異無(wú)統(tǒng)計(jì)學(xué)意義（χ2=0.833，P=0.842）； （3）Ⅰ~Ⅱ期的陽(yáng)性表達(dá)率為35.3%（6/17），低于Ⅲ~Ⅳ期75.0%（21/28），差異有統(tǒng)計(jì)學(xué)意義（χ2=6.949，P=0.008）； （4）高分化（G1）組的陽(yáng)性表達(dá)率為12.5%（l/8），明顯低于中（G2）、低分化（G3）組70.3%（26/37），差異具有統(tǒng)計(jì)學(xué)意義（χ2=6.898，P=0.009）； （5）有淋巴結(jié)轉(zhuǎn)移組的陽(yáng)性表達(dá)率為84.2%（16/19），，高于無(wú)淋巴結(jié)轉(zhuǎn)移組42.3%（11/26），差異具有統(tǒng)計(jì)學(xué)意義（χ2=8.031，P=0.005）。 （6）有腹水組的陽(yáng)性表達(dá)率為64.3%（9/14），無(wú)腹水組的陽(yáng)性表達(dá)率為58.2%(18/31)，兩者比較差異無(wú)統(tǒng)計(jì)學(xué)意義（χ2=0.004，P=0.948）。 5、B7-H4蛋白和HK6蛋白的表達(dá)呈低度線性正相關(guān)關(guān)系（r=0.218，P=0.039）。 結(jié)論 1、B7-H4蛋白和HK6蛋白在卵巢上皮性腫瘤組織中均較高水平表達(dá)，且表達(dá)強(qiáng)度隨病理分期增加而增強(qiáng)，提示B7-H4蛋白和HK6蛋白可能參與卵巢上皮性腫瘤的進(jìn)展。 2、在卵巢上皮性惡性腫瘤組織中B7-H4蛋白和HK6蛋白的表達(dá)程度均與病理分期、病理分級(jí)及淋巴結(jié)轉(zhuǎn)移有關(guān)，而與患者年齡及腹水無(wú)關(guān)。B7-H4蛋白的表達(dá)還與組織學(xué)類型有關(guān)，HK6蛋白的表達(dá)則與之無(wú)關(guān)。 3、B7-H4蛋白和HK6蛋白的表達(dá)間存在一定的線性正相關(guān)關(guān)系，說(shuō)明B7-H4蛋白和HK6蛋白可能協(xié)同參與了卵巢上皮性惡性腫瘤的發(fā)生和發(fā)展。
[Abstract]:objective
The expression of B7-H4 protein and HK6 protein in normal ovarian tissues, ovarian epithelial benign tumor tissues, borderline tumor tissues and malignant tumor tissues were detected to explore the possible role of B7-H4 protein and HK6 protein in the occurrence and development of epithelial ovarian tumors and its clinical significance.
The expression of B7-H4 protein and HK6 protein in 70 cases of ovarian epithelial tumors and 10 cases of normal ovarian tissues were detected by immunohistochemical staining SP method. The positive expression of B7-H4 protein and HK6 protein in normal ovarian tissues, ovarian epithelial benign, borderline and malignant ovarian tissues were compared. Statistical analysis was made on the relationship between the expression of the protein in ovarian epithelial malignant tumors and the clinicopathological parameters.
Result
1. The positive rates of B7-H4 protein in normal ovarian tissues, benign epithelial ovarian tissues, borderline ovarian tissues and malignant tumors were 0.0% (0/10), 20.0% (3/15), 30.0% (3/10) and 80.0% (36/45), respectively. The positive rates in ovarian epithelial malignant tumors were significantly higher than those in the first three groups (2 = 31.192, P = 0.000). There was no significant difference between benign tumor group and borderline tumor group (x 2=0.009, P=0.924).
2, the expression of B7-H4 protein in epithelial ovarian cancer tissues:
(1) The positive expression rate was 75.0% (6/8) in the group under 50 years old and 81.1% (30/37) in the group over 50 years old. There was no significant difference between the two groups (_2 = 0.000, P = 1.000).
(2) The positive expression rates in serous carcinoma, mucinous carcinoma, endometrioid carcinoma and clear cell carcinoma were 96.2% (25/26), 28.6% (2/7), 83.8% (5/6) and 66.7% (4/6), respectively. The positive expression rates in mucinous carcinoma group were 28.6% (2/7), significantly lower than those in non-mucinous carcinoma group (89.5% (34/38), and the difference was statistically significant (2 = 10.161, P = 0.001). There was no significant difference among groups (chi 2=4.052, P=0.132).
(3) The positive expression rate of stage I-II was 52.9% (9/17), significantly lower than that of stage III-IV (96.5% (27/28), and the difference was statistically significant (_2 = 9.932, P = 0.002).
(4) The positive expression rate in G1 group was 37.5% (3/8), significantly lower than that in G2 group and 89.2% (33/37) in G3 group. The difference was statistically significant (2 = 10.984, P = 0.001).
(5) The positive rate of lymph node metastasis group was 94.7% (18/19), which was higher than that of non-lymph node metastasis group (69.2% (18/26), and the difference was statistically significant (_2 = 4.464, P = 0.035).
(6) The positive expression rate was 85.7% (12/14) in ascites group and 77.4% (24/31) in non-ascites group. There was no significant difference between the two groups (_2 = 0.058, P = 0.809).
3. The positive rates of HK6 protein in normal ovarian tissues, benign epithelial ovarian tissues, borderline ovarian tissues and malignant tumors were 10.0% (1/10), 13.3% (2/15), 50.0% (5/10) and 60.0% (27/45), respectively. The positive rates of HK6 protein in benign ovarian epithelial tumors were significantly lower than those in borderline ovarian tissues and malignant ovarian tumors (2=9). There was no significant difference between the borderline tumor group and the malignant tumor group (_2 = 0.051, P = 0.822).
4, the expression of HK6 protein in ovarian cancer tissues:
(1) The positive expression rate was 62.5% (5/8) in the group under 50 years old and 59.5% (22/37) in the group over 50 years old. There was no significant difference between the two groups (_2 = 0.000, P = 1.000).
(2) The positive expression rates of serous carcinoma, mucinous carcinoma, endometrioid carcinoma and clear cell carcinoma were 65.4% (17/26), 57.1% (4/7), 50.0% (3/6) and 50.0% (3/6), respectively. There was no significant difference between different histological types (2 = 0.833, P = 0.842).
(3) The positive rate of stage I-II was 35.3% (6/17), lower than 75.0% (21/28) in stage III-IV, and the difference was statistically significant (_2 = 6.949, P = 0.008).
(4) The positive expression rate of G1 group was 12.5% (l/8), significantly lower than that of G2 group and 70.3% (26/37) in G3 group. The difference was statistically significant (2 = 6.898, P = 0.009).
(5) The positive rate of lymph node metastasis group was 84.2% (16/19), which was higher than that of non-lymph node metastasis group (42.3% (11/26), and the difference was statistically significant (_2 = 8.031, P = 0.005).
(6) The positive expression rate was 64.3% (9/14) in ascites group and 58.2% (18/31) in non-ascites group. There was no significant difference between the two groups (_2 = 0.004, P = 0.948).
5, there was a low positive linear correlation between B7-H4 protein and HK6 protein (r=0.218, P=0.039).
conclusion
1, B7-H4 protein and HK6 protein were highly expressed in ovarian epithelial tumors, and the expression intensity increased with the pathological stage, suggesting that B7-H4 protein and HK6 protein may be involved in the progression of ovarian epithelial tumors.
2. The expression of B7-H4 and HK6 proteins in ovarian epithelial malignant tumors was correlated with pathological stage, pathological grade and lymph node metastasis, but not with age and ascites. The expression of B7-H4 protein was correlated with histological type, but not with HK6 protein.
3. There is a linear positive correlation between the expression of B7-H4 protein and HK6 protein, indicating that B7-H4 protein and HK6 protein may be involved in the occurrence and development of ovarian epithelial malignancies.
【學(xué)位授予單位】：泰山醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.31
本文編號(hào)：2218762
[Abstract]:objective
The expression of B7-H4 protein and HK6 protein in normal ovarian tissues, ovarian epithelial benign tumor tissues, borderline tumor tissues and malignant tumor tissues were detected to explore the possible role of B7-H4 protein and HK6 protein in the occurrence and development of epithelial ovarian tumors and its clinical significance.
The expression of B7-H4 protein and HK6 protein in 70 cases of ovarian epithelial tumors and 10 cases of normal ovarian tissues were detected by immunohistochemical staining SP method. The positive expression of B7-H4 protein and HK6 protein in normal ovarian tissues, ovarian epithelial benign, borderline and malignant ovarian tissues were compared. Statistical analysis was made on the relationship between the expression of the protein in ovarian epithelial malignant tumors and the clinicopathological parameters.
Result
1. The positive rates of B7-H4 protein in normal ovarian tissues, benign epithelial ovarian tissues, borderline ovarian tissues and malignant tumors were 0.0% (0/10), 20.0% (3/15), 30.0% (3/10) and 80.0% (36/45), respectively. The positive rates in ovarian epithelial malignant tumors were significantly higher than those in the first three groups (2 = 31.192, P = 0.000). There was no significant difference between benign tumor group and borderline tumor group (x 2=0.009, P=0.924).
2, the expression of B7-H4 protein in epithelial ovarian cancer tissues:
(1) The positive expression rate was 75.0% (6/8) in the group under 50 years old and 81.1% (30/37) in the group over 50 years old. There was no significant difference between the two groups (_2 = 0.000, P = 1.000).
(2) The positive expression rates in serous carcinoma, mucinous carcinoma, endometrioid carcinoma and clear cell carcinoma were 96.2% (25/26), 28.6% (2/7), 83.8% (5/6) and 66.7% (4/6), respectively. The positive expression rates in mucinous carcinoma group were 28.6% (2/7), significantly lower than those in non-mucinous carcinoma group (89.5% (34/38), and the difference was statistically significant (2 = 10.161, P = 0.001). There was no significant difference among groups (chi 2=4.052, P=0.132).
(3) The positive expression rate of stage I-II was 52.9% (9/17), significantly lower than that of stage III-IV (96.5% (27/28), and the difference was statistically significant (_2 = 9.932, P = 0.002).
(4) The positive expression rate in G1 group was 37.5% (3/8), significantly lower than that in G2 group and 89.2% (33/37) in G3 group. The difference was statistically significant (2 = 10.984, P = 0.001).
(5) The positive rate of lymph node metastasis group was 94.7% (18/19), which was higher than that of non-lymph node metastasis group (69.2% (18/26), and the difference was statistically significant (_2 = 4.464, P = 0.035).
(6) The positive expression rate was 85.7% (12/14) in ascites group and 77.4% (24/31) in non-ascites group. There was no significant difference between the two groups (_2 = 0.058, P = 0.809).
3. The positive rates of HK6 protein in normal ovarian tissues, benign epithelial ovarian tissues, borderline ovarian tissues and malignant tumors were 10.0% (1/10), 13.3% (2/15), 50.0% (5/10) and 60.0% (27/45), respectively. The positive rates of HK6 protein in benign ovarian epithelial tumors were significantly lower than those in borderline ovarian tissues and malignant ovarian tumors (2=9). There was no significant difference between the borderline tumor group and the malignant tumor group (_2 = 0.051, P = 0.822).
4, the expression of HK6 protein in ovarian cancer tissues:
(1) The positive expression rate was 62.5% (5/8) in the group under 50 years old and 59.5% (22/37) in the group over 50 years old. There was no significant difference between the two groups (_2 = 0.000, P = 1.000).
(2) The positive expression rates of serous carcinoma, mucinous carcinoma, endometrioid carcinoma and clear cell carcinoma were 65.4% (17/26), 57.1% (4/7), 50.0% (3/6) and 50.0% (3/6), respectively. There was no significant difference between different histological types (2 = 0.833, P = 0.842).
(3) The positive rate of stage I-II was 35.3% (6/17), lower than 75.0% (21/28) in stage III-IV, and the difference was statistically significant (_2 = 6.949, P = 0.008).
(4) The positive expression rate of G1 group was 12.5% (l/8), significantly lower than that of G2 group and 70.3% (26/37) in G3 group. The difference was statistically significant (2 = 6.898, P = 0.009).
(5) The positive rate of lymph node metastasis group was 84.2% (16/19), which was higher than that of non-lymph node metastasis group (42.3% (11/26), and the difference was statistically significant (_2 = 8.031, P = 0.005).
(6) The positive expression rate was 64.3% (9/14) in ascites group and 58.2% (18/31) in non-ascites group. There was no significant difference between the two groups (_2 = 0.004, P = 0.948).
5, there was a low positive linear correlation between B7-H4 protein and HK6 protein (r=0.218, P=0.039).
conclusion
1, B7-H4 protein and HK6 protein were highly expressed in ovarian epithelial tumors, and the expression intensity increased with the pathological stage, suggesting that B7-H4 protein and HK6 protein may be involved in the progression of ovarian epithelial tumors.
2. The expression of B7-H4 and HK6 proteins in ovarian epithelial malignant tumors was correlated with pathological stage, pathological grade and lymph node metastasis, but not with age and ascites. The expression of B7-H4 protein was correlated with histological type, but not with HK6 protein.
3. There is a linear positive correlation between the expression of B7-H4 protein and HK6 protein, indicating that B7-H4 protein and HK6 protein may be involved in the occurrence and development of ovarian epithelial malignancies.
【學(xué)位授予單位】：泰山醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.31
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2 谷文文;杜丹麗;;HK6和B7-H4在卵巢癌診斷價(jià)值的探討[J];蚌埠醫(yī)學(xué)院學(xué)報(bào);2012年06期
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