【摘要】：目的:研究臨床致病白念珠菌交配型基因MTL純合子與雜合子的流行病學(xué)特點,分析其與外陰陰道念珠菌病患者的臨床特征、白念珠菌致病力及毒力的關(guān)系。方法:通過普通PCR對臨床外陰陰道念珠菌病患者陰道分泌物分離的致病白念珠菌MTL基因進(jìn)行檢測,應(yīng)用實時熒光定量PCR反應(yīng)分析MTL純合子與雜合子中毒力相關(guān)基因ASL3、SAP1和HWP1基因的表達(dá),并采用Rosco藥片擴(kuò)散法對白念珠菌進(jìn)行體外藥物敏感試驗。結(jié)果:1338株白念珠菌中,1330株為MTLa/α雜合子,8株為MTL純合子(7株MTLa/a純合子,1株MTLα/α純合子)。8株MTL純合子所致外陰陰道念珠菌病患者中6例患者達(dá)到真菌學(xué)治愈。與雜合子相比,白念珠菌MTL純合子對唑類藥物耐藥性較高(0~75%vs 0~2.7%,P0.05)。毒力相關(guān)基因ASL3在兩者中的表達(dá)無明顯統(tǒng)計學(xué)差異,SAP1在MTL雜合子中表達(dá)稍高(1.02±0.68 vs 0.83±0.31,P0.05),而HWP1在雜合子中表達(dá)明顯增高(1.71±1.42 vs 1.20±0.62,P0.05)。結(jié)論:外陰陰道念珠菌病白念珠菌MTL純合子在外陰陰道念珠菌病患者中流行率較低;與雜合子相比,MTL純合子耐藥性增加;而毒力相關(guān)基因HWP1、SAP1和ASL3在純合子中表達(dá)降低,這與其臨床特征相符。
[Abstract]:Objective: to study the epidemiological characteristics of MTL homozygote and heterozygote of clinical pathogenic Candida albicans mating gene, and to analyze its relationship with the clinical characteristics, pathogenicity and virulence of Candida albicans in patients with vulvovaginal candidiasis. Methods: the MTL gene of Candida albicans isolated from vaginal secretions of patients with vulvovaginal candidiasis was detected by ordinary PCR. The expression of MTL homozygous and heterozygous genes ASL3nSAP1 and HWP1 were analyzed by real-time fluorescence quantitative PCR reaction. The drug sensitivity of Candida albicans was tested by Rosco tablet diffusion method in vitro. Results among the 13 38 strains of Candida albicans, 1 330 were MTLa/ 偽 heterozygotes and 8 were MTL homozygotes (7 MTLa/a homozygotes 1 MTL 偽 / 偽 homozygote). 6 of 8 cases of vulvovaginal candidiasis caused by MTL homozygotes achieved mycological cure. Compared with heterozygote, MTL homozygote of Candida albicans was more resistant to azolium than that of heterozygote (P 0.05). There was no significant difference in the expression of virulence related gene ASL3 between them. The expression of SAP1 in MTL heterozygote was slightly higher than that in heterozygote (1.02 鹵0.68 vs 0.83 鹵0.31 P 0.05), while the expression of HWP1 in heterozygote was significantly higher (1.71 鹵1.42 vs 1.20 鹵0.62P 0.05). Conclusion: the prevalence rate of MTL homozygotes in vulvovaginal candidiasis patients is lower than that of heterozygotes, while the expression of virulence related genes HWP1SAP1 and ASL3 in the homozygotes is lower than that in heterozygotes. This is consistent with its clinical features.
【作者單位】： 北京大學(xué)深圳醫(yī)院婦產(chǎn)科;北京大學(xué)深圳醫(yī)院檢驗科;深圳市科創(chuàng)委婦科疾病重點實驗室;
【基金】：深圳科創(chuàng)委基礎(chǔ)研究項目(No:201201001和JCYJ20140415162338813)
【分類號】：R711.31

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 陳雪蓉;肖敦振;;白念珠菌毒力因子的研究進(jìn)展[J];中國婦幼保健;2007年32期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 羅紅梅;苑天紅;佘曉玲;賀娟;譚心;馬研;;高效提取二相性白假絲酵母菌總RNA實驗研究[J];貴陽醫(yī)學(xué)院學(xué)報;2012年06期

2 易佳麗;劉亞莉;劍輝;劉軼群;;白色念珠菌胃潰瘍分離株和胃癌分離株的毒力及磷脂酶活性差異的實驗研究[J];中國醫(yī)藥科學(xué);2014年20期

3 唐莉;隗伏冰;汪珊;;妊娠合并外陰陰道假絲酵母菌病的菌種分布的相關(guān)研究[J];婦產(chǎn)與遺傳(電子版);2014年02期

4 夏艷;陸琴;陳美新;金志軍;;復(fù)發(fā)性假絲酵母菌性外陰陰道炎與腸道假絲酵母菌同源感染患者的臨床治療研究[J];國際婦產(chǎn)科學(xué)雜志;2015年02期

5 王雪連;孔慶濤;劉芳;沈永年;王高峰;馮姣;黨潔明;桑紅;;女性外陰瘙癢與白念珠菌超敏反應(yīng)的關(guān)系[J];醫(yī)學(xué)研究生學(xué)報;2014年03期

6 張麗娟;寧玉梅;;妊娠期外陰陰道假絲酵母菌病的研究進(jìn)展[J];中國計劃生育學(xué)雜志;2014年08期

7 周銀娣;溫和;;陰道炎診斷的研究進(jìn)展[J];檢驗醫(yī)學(xué)與臨床;2014年18期

8 王曉燕;齊眉;李玉瑭;孫允東;張曉麗;劉娟;程軼U，

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