【摘要】：目的利用公共數(shù)據(jù)庫分析卵巢癌組織中12種micro RNA(mi R)的表達及其臨床意義。方法篩選在GSE14407數(shù)據(jù)集和TCGA數(shù)據(jù)庫中均具有表達信息的micro RNA,得到mi R-10B、mi R-1244、mi R-622、mi R-21、mi R-503、Let-7D、mi R-155、mi R-30C、mi R-17、mi R-101-1、mi R-186以及mi R-770共12種micro RNA,在這12種micro RNA中尋找在卵巢癌(卵巢癌組)和癌旁組織(癌旁組)中表達有差異的基因。利用在TCGA數(shù)據(jù)庫中篩選得到的505例卵巢癌患者數(shù)據(jù),年齡按照中位數(shù)分為高年齡(≥59歲)組和低年齡(59歲)組;臨床分期分為早期組(Ⅰ期+Ⅱ期)、晚期組(Ⅲ期+Ⅳ期)及未知;腫瘤分級分為低級別(G1+G2)組和高級別(G3+G4)組;病發(fā)位置分為單側(cè)組、雙側(cè)組;腫瘤殘余分為10 mm組、≥10 mm組;micro RNA按照表達量的中位數(shù)分為高表達組和低表達組,進行Kaplan-Meier單因素生存分析和多因素Cox回歸分析。結(jié)果癌旁組Let-7D和mi R-101-1表達水平高于卵巢癌組,而mi R-155和mi R-770表達水平低于卵巢癌組(均P0.05)。生存分析顯示,年齡≥59歲、臨床分期(Ⅲ+Ⅳ)期和Let-7D低表達的患者生存情況較差(P0.05)。Let-7D低表達是卵巢癌患者生存的獨立危險因素。結(jié)論 Let-7D的表達與卵巢癌預后相關,可能是卵巢癌預后的獨立影響指標。
[Abstract]:Objective to analyze the expression of 12 kinds of micro RNA (mi R) and its clinical significance in ovarian cancer by using public database. Methods micro RNAs expressing information in GSE14407 data set and TCGA database were screened, and 12 micro RNAs were obtained in the 12 micro RNA types: mi R-10BPU mi R-1244N mi R-622mmi R-222mmi R-503n R-503n R-155Cnmi R-30Cnmi R-17Cmmi R-101-1mmi R-186 and mi R-770 micro RNAs. Among the 12 micro RNA RNAs, 12 micro RNAs were found in ovarian cancer (ovarian cancer group) and paracarcinomatous group. Differentially expressed genes are expressed in the tissue (paracancerous group). According to the data of 505 ovarian cancer patients screened in TCGA database, age was divided into high age group (鈮，

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