【摘要】：目的：本文旨在通過檢測(cè)PDCD5 (programmed cell death 5,程序性細(xì)胞死亡因子5)在子宮內(nèi)膜癌(Endometrial carcinoma, EC)組織中的表達(dá)水平,結(jié)合臨床資料,并分析其意義,初步探討PDCD5的表達(dá)與子宮內(nèi)膜癌發(fā)生的相關(guān)性。方法：收集2012年5月至2013年2月于濱州醫(yī)學(xué)院附屬醫(yī)院婦科行婦科手術(shù),且均經(jīng)病理學(xué)檢查證實(shí)為EC為研究對(duì)象,總標(biāo)本例數(shù)n=60；另隨機(jī)選取同期行婦科手術(shù)中所取得子宮內(nèi)膜不典型增生(endometrial atypical hyperplasia, EAH)組織(n=28)和正常增生期子宮內(nèi)膜(normal proliferative endometrium, NPE)組織(n=28)作為對(duì)照。分別采用實(shí)時(shí)熒光定量PCR(Realtime fluores-cence quantitative PCR, Real time PCR)和免疫組織化學(xué)法(Immunohistochemical, IHC)檢測(cè)PDCD5 mRNA和蛋白的表達(dá)水平,并分析其與子宮內(nèi)膜癌病理特征的關(guān)系。本實(shí)驗(yàn)所遵守的標(biāo)準(zhǔn)為濱州醫(yī)學(xué)院附屬醫(yī)院倫理委員會(huì)所制定的相關(guān)倫理學(xué)標(biāo)準(zhǔn)。結(jié)果：子宮內(nèi)膜癌中PDCD5 mRNA的表達(dá)明顯低于對(duì)照組,且差異有統(tǒng)計(jì)學(xué)意義,P≤0.05。PDCD5蛋白在EC組織中的陽性表達(dá)率為45.0%,顯著低于EAH及NPE組織中的陽性表達(dá)率(P≤0.05)。而且結(jié)果顯示PDCD5的表達(dá)水平和EC患者的臨床分期、組織學(xué)分級(jí)、肌層浸潤(rùn)以及有無淋巴結(jié)轉(zhuǎn)移有相關(guān)性(均P≤0.05),而與子宮內(nèi)膜癌患者的年齡未發(fā)現(xiàn)明顯的關(guān)聯(lián)性(P0.05)。結(jié)論:采用實(shí)時(shí)熒光定量PCR和免疫組織化學(xué)技術(shù)檢測(cè)子宮內(nèi)膜癌組織中PDCD5的表達(dá),發(fā)現(xiàn)PDCD5在子宮內(nèi)膜癌組織中的表達(dá)較對(duì)照組織中的表達(dá)降低甚至缺失。且PDCD5的表達(dá)水平與子宮內(nèi)膜癌的臨床病理參數(shù)密切相關(guān)：臨床分期越高,PDCD5表達(dá)越低；組織學(xué)分級(jí)越高,PDCD5蛋白的表達(dá)越低；肌層浸潤(rùn)越深,PDCD5表達(dá)越低；淋巴結(jié)有轉(zhuǎn)移,PDCD5表達(dá)越低；PDCD5表達(dá)與年齡無關(guān)。PDCD5有可能成為一種潛在的子宮內(nèi)膜癌的早期診斷分子標(biāo)記物。
[Abstract]:Objective: to investigate the expression of PDCD5 (programmed cell death 5 and programmed cell death factor 5 (PDCD5 (programmed cell death 5) in endometrial carcinoma (Endometrial carcinoma, EC) and to analyze their clinical significance. To explore the correlation between the expression of PDCD5 and the occurrence of endometrial carcinoma. Methods: from May 2012 to February 2013, gynecological operations were performed in affiliated Hospital of Binzhou Medical College, and were confirmed as EC by pathological examination. Another randomly selected endometrial atypical hyperplasia (endometrial atypical hyperplasia, EAH) tissue (nm28) and normal proliferative endometrial (normal proliferative endometrium, NPE) tissue (nm28) obtained during gynecological surgery were used as control group. The expression of PDCD5 mRNA and protein was detected by real-time fluorescence quantitative PCR (Realtime fluores-cence quantitative PCR, Real time PCR) and immunohistochemical (Immunohistochemical, IHC), and the relationship between the expression of PDCD5 mRNA and the pathological features of endometrial carcinoma was analyzed. The standard of this experiment is the ethical standard of the affiliated Hospital Ethics Committee of Binzhou Medical College. Results: the expression of PDCD5 mRNA in endometrial carcinoma was significantly lower than that in control group, and the positive expression rate of P 鈮，

本文編號(hào)：2172141