【摘要】：目的:研究大鼠孕期鉛暴露后子代海馬突觸變化與PSD-95表達改變的相關性,探討鉛暴露損傷學習記憶功能的機制。方法:通過飲用0.02%醋酸鉛水溶液建立孕期鉛暴露模型,利用原子吸收分光光度儀檢測血鉛,透射電鏡技術檢測海馬突觸密度,蛋白印記技術檢測子代大鼠海馬組織中VGLUT、VGAT和PSD-95的表達。結果:對照組和鉛暴露組雄性21 d大鼠體重分別為(55.73±4.23)g和(56.01±5.97)g,無顯著差異(P0.05);對照組與鉛暴露組雄性21 d大鼠血鉛分別為(10.2±2.1)μg/L和(301.2±34.8)μg/L,血鉛水平明顯升高(P0.01);對照組與鉛暴露組雄性21 d大鼠單位面積突觸數(shù)目分別為32.79±2.03和23.46±1.97,突觸密度明顯減少(P0.01);鉛暴露后,VGAT的表達量沒有明顯變化(P0.05),VGLUT和PSD-95的表達量明顯減少(P0.01)。結論:PSD-95表達減少引起海馬興奮性突觸數(shù)目的下降是發(fā)育期鉛暴露損傷學習記憶功能的可能機制。
[Abstract]:Aim: to study the relationship between the changes of hippocampal synapses and PSD-95 expression in offspring after lead exposure during pregnancy and to explore the mechanism of damage to learning and memory function by lead exposure. Methods: lead exposure model was established by drinking 0.02% lead acetate solution during pregnancy. Blood lead was detected by atomic absorption spectrophotometer and synaptic density of hippocampus was measured by transmission electron microscopy. Protein imprinting technique was used to detect the expression of VGLUTT VGAT and PSD- 95 in rat hippocampus. Results: the body weight of male rats in the control group and lead exposure group were (55.73 鹵4.23) g and (56.01 鹵5.97) g, respectively (P0.05), and the blood lead levels in the control group and lead exposure group were (10.2 鹵2.1) 渭 g / L and (301.2 鹵34.8) 渭 g / L, respectively (P0.01), while those in the control group and lead exposure group were significantly increased (P0.01). The number of synapses per unit area of male rats was 32.79 鹵2.03 and 23.46 鹵1.97, respectively, and the synaptic density decreased significantly (P0.01), while the expression of VGAT and PSD-95 decreased significantly after lead exposure (P0.05). Conclusion the decrease of the number of excitatory synapses induced by the decrease of the expression of PSD-95 in the hippocampus is the possible mechanism of the impairment of learning and memory function by lead exposure during development.
【作者單位】： 第四軍醫(yī)大學軍事預防醫(yī)學院軍隊勞動與環(huán)境衛(wèi)生學教研室;
【基金】：國家重點基礎研究發(fā)展計劃(973計劃,2012CD525002) 國家自然科學基金(81273101)
【分類號】：R714.2

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