PDCD5與XIAP蛋白在子宮腺肌病中的表達及意義
發(fā)布時間：2018-07-13 19:42
【摘要】：目的：探討凋亡及其相關因子程序性細胞死亡分子5(programmed cell death5, PDCD5)與X-連鎖調(diào)亡抑制蛋白(X-linkedinhibitor of apoptosis protein, XIAP)在子宮腺肌病(adenomyosis, AM)發(fā)病中的作用，為AM的臨床診治及深入研究提供實驗依據(jù)。方法：收集子宮全切術患者子宮標本，篩選出其中經(jīng)病理確診為AM的標本共30例，取其異位區(qū)內(nèi)膜組織（異位組）和非異位區(qū)內(nèi)膜組織（在位組）；同期收集并篩選出其中經(jīng)病理確診僅為子宮肌瘤的子宮標本共30例，取其非肌瘤區(qū)內(nèi)膜組織作為對照組。所有入選實驗對象在術前半年內(nèi)均未服用激素類和抗子宮腺肌病藥物。采用原位末端標記（TdT-mediated dUTP Nick-End Labeling, Tunel）細胞凋亡檢測試劑盒檢測三組內(nèi)膜腺上皮細胞凋亡情況，計數(shù)各組內(nèi)膜腺上皮細胞凋亡指數(shù)(apoptosis index,AI)。免疫組化法檢測PDCD5與XIAP在三組內(nèi)膜組織中的表達，應用Image Pro6.0專業(yè)圖像分析系統(tǒng)分析PDCD5與XIAP陽性細胞平均光密度值（mean optical density, MOD），分析二者在三組內(nèi)膜腺上皮細胞中的表達差異及相關性。實驗數(shù)據(jù)采用SPSS17.0軟件進行統(tǒng)計學分析，P0.05為差異有統(tǒng)計學意義。結果：1、異位組內(nèi)膜細胞凋亡指數(shù)AI略小于在位組，差異無統(tǒng)計學意義（P＞0.05），明顯小于對照組且差異有統(tǒng)計學意義(P0.05)；在位組內(nèi)膜細胞AI明顯小于對照組，差異有統(tǒng)計學意義(P0.05)。2、PDCD5在三組內(nèi)膜中的表達，異位組明顯低于對照組，差異有統(tǒng)計學意義(P0.05)；在位組低于對照組，差異有統(tǒng)計學意義（P0.05）；在位組略低于異位組，差異無統(tǒng)計學意義（P＞0.05）。3、XIAP在三組內(nèi)膜中的表達，異位組明顯高于對照組，差異有統(tǒng)計學意義(P0.05)；在位組高于對照組，差異有統(tǒng)計學意義（P0.05）在位組略低于異位組，，差異無統(tǒng)計學意義（P＞0.05）4、相關性：PDCD5與XIAP在異位組呈負相關（r=－0.415, P=0.0230.05），在在位組呈負相關（r=-0.382, P=0.0370.05），在對照組呈不相關(r=－0.126,P=0.5060.05)。結論：1、與對照組相比，異位組和在位組內(nèi)膜細胞凋亡指數(shù)AI都明顯減小，說明在AM的形成過程中可能確實存在內(nèi)膜細胞的凋亡異常，這種異常可能是直接或間接參與形成AM的重要因素之一。2、與對照組相比，異位組和在位組中促凋亡蛋白PDCD5與凋亡抑制蛋白XIAP的表達都存在明顯差異，PDCD5表達的明顯下調(diào)及XIAP表達的明顯上調(diào)，提示PDCD5和XIAP的表達異�？赡苁谴俪葾M患者子宮內(nèi)膜細胞出現(xiàn)凋亡異�，F(xiàn)象的重要原因之一，是參與AM發(fā)病并最終促使AM形成的重要原因之一。3、PDCD5與XIAP在異位和在位內(nèi)膜組織中的表達均呈負相關，提示二者存在明顯的相互拮抗或負反饋調(diào)節(jié)關系。此外，三組內(nèi)膜組織中，異位組中PDCD5的表達最低，XIAP的表達最高，這進一步表明PDCD5與XIAP的這種相互拮抗或負反饋調(diào)節(jié)作用在異位內(nèi)膜組織中表現(xiàn)得更為明顯，提示二者在內(nèi)膜細胞中表達異常導致的相互作用失衡可能是異位內(nèi)膜細胞能適應異位區(qū)環(huán)境并在其中生長，從而進一步促進AM發(fā)生發(fā)展的關鍵因素之一。
[Abstract]:Objective: To explore the role of apoptosis and its related factors, programmed cell death molecule 5 (programmed cell death5, PDCD5) and X- linked fall suppression protein (X-linkedinhibitor of apoptosis protein, XIAP) in the pathogenesis of adenomyosis (adenomyosis, AM), and provide experimental basis for clinical diagnosis and treatment and in-depth study. A total of 30 cases of uterine specimens with hysterectomy were selected and 30 specimens were confirmed by pathology. The endometrium endometrium (ectopic tissue) and non ectopic endometrium tissue (incumbent group) were selected. 30 cases of uterine specimens, which were pathologically diagnosed as myoma of uterus, were collected and screened in the same period. The non myoma endometrium tissue was taken as the control group. The three groups of endometrial gland epithelial cells apoptosis were detected by the TdT-mediated dUTP Nick-End Labeling (Tunel) cell apoptosis detection kit, and the apoptosis index (apoptosis index, AI) of the endometrium epithelial cells (apoptosis index, AI) was counted. The expression of PDCD5 and XIAP in the three groups of endometrium was detected by the immunohistochemical method. The average optical density value of PDCD5 and XIAP positive cells (mean optical density, MOD) was analyzed by the Image Pro6.0 professional image analysis system. The difference and correlation between the two groups in the three groups of endometrial gland epithelial cells were analyzed. The experimental data were carried out by SPSS17.0 software. The results of P0.05 were statistically significant. Results: 1, the apoptotic index AI of endometrium cells in ectopic group was slightly smaller than that of the incumbent group (P > 0.05), which was significantly smaller than that in the control group (P0.05), and the AI in the eutopic endometrium was significantly smaller than that in the control group (P0.05).2, PDCD5 in three groups. The expression of the membrane in the ectopic group was significantly lower than that in the control group (P0.05), and the difference was statistically significant (P0.05) in the incumbent group. The difference was slightly lower than the ectopic group (P > 0.05).3, and XIAP was in the three groups of endometrium, and the ectopic group was significantly higher than the control group, the difference was statistically significant (P0.05). The difference was significantly higher than that of the control group (P0.05), the difference was slightly lower than that of the ectopic group (P > 0.05) 4, and the correlation was negative correlation between PDCD5 and XIAP (r= 0.415, P=0.0230.05) in the ectopic group (r=-0.382, P=0.0370.05) and in the control group (r = - 0. 12 6, P=0.5060.05). 1, 1, compared with the control group, the apoptotic index of the endometrium cells in the ectopic and the incumbent groups decreased obviously, indicating that the apoptosis of the endometrium may be abnormal in the formation of AM, which may be one of the important factors to directly or indirectly participate in the formation of AM,.2, which is compared with those in the group, and the apoptotic protein PD in the ectopic group and the incumbent group. There are obvious differences in the expression of CD5 and apoptosis inhibitory protein XIAP, the obvious downregulation of PDCD5 expression and the obvious up-regulation of the expression of XIAP, suggesting that the abnormal expression of PDCD5 and XIAP may be one of the important reasons for the abnormal phenomenon of apoptosis in endometrium cells of AM patients. It is one of the important reasons for participating in AM hair disease and ultimately contributing to the formation of AM.3, P. The expression of DCD5 and XIAP in ectopic and eutopic endometrium was negatively correlated, suggesting that there were obvious antagonism or negative feedback regulation between the two groups. In addition, the expression of PDCD5 was the lowest in the three group of endometrium tissues, and the expression of XIAP was the highest. This further indicated that the mutual antagonism or negative feedback regulation of PDCD5 and XIAP was ectopic and negative feedback. The expression of endometrium in endometrium is more obvious, suggesting that the imbalance in the expression of two of the endometrium cells may be one of the key factors that can further promote the development of AM by the ectopic endometrium cells that can adapt to the ectopic environment and grow in it.
【學位授予單位】：瀘州醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R711.71
本文編號：2120534
[Abstract]:Objective: To explore the role of apoptosis and its related factors, programmed cell death molecule 5 (programmed cell death5, PDCD5) and X- linked fall suppression protein (X-linkedinhibitor of apoptosis protein, XIAP) in the pathogenesis of adenomyosis (adenomyosis, AM), and provide experimental basis for clinical diagnosis and treatment and in-depth study. A total of 30 cases of uterine specimens with hysterectomy were selected and 30 specimens were confirmed by pathology. The endometrium endometrium (ectopic tissue) and non ectopic endometrium tissue (incumbent group) were selected. 30 cases of uterine specimens, which were pathologically diagnosed as myoma of uterus, were collected and screened in the same period. The non myoma endometrium tissue was taken as the control group. The three groups of endometrial gland epithelial cells apoptosis were detected by the TdT-mediated dUTP Nick-End Labeling (Tunel) cell apoptosis detection kit, and the apoptosis index (apoptosis index, AI) of the endometrium epithelial cells (apoptosis index, AI) was counted. The expression of PDCD5 and XIAP in the three groups of endometrium was detected by the immunohistochemical method. The average optical density value of PDCD5 and XIAP positive cells (mean optical density, MOD) was analyzed by the Image Pro6.0 professional image analysis system. The difference and correlation between the two groups in the three groups of endometrial gland epithelial cells were analyzed. The experimental data were carried out by SPSS17.0 software. The results of P0.05 were statistically significant. Results: 1, the apoptotic index AI of endometrium cells in ectopic group was slightly smaller than that of the incumbent group (P > 0.05), which was significantly smaller than that in the control group (P0.05), and the AI in the eutopic endometrium was significantly smaller than that in the control group (P0.05).2, PDCD5 in three groups. The expression of the membrane in the ectopic group was significantly lower than that in the control group (P0.05), and the difference was statistically significant (P0.05) in the incumbent group. The difference was slightly lower than the ectopic group (P > 0.05).3, and XIAP was in the three groups of endometrium, and the ectopic group was significantly higher than the control group, the difference was statistically significant (P0.05). The difference was significantly higher than that of the control group (P0.05), the difference was slightly lower than that of the ectopic group (P > 0.05) 4, and the correlation was negative correlation between PDCD5 and XIAP (r= 0.415, P=0.0230.05) in the ectopic group (r=-0.382, P=0.0370.05) and in the control group (r = - 0. 12 6, P=0.5060.05). 1, 1, compared with the control group, the apoptotic index of the endometrium cells in the ectopic and the incumbent groups decreased obviously, indicating that the apoptosis of the endometrium may be abnormal in the formation of AM, which may be one of the important factors to directly or indirectly participate in the formation of AM,.2, which is compared with those in the group, and the apoptotic protein PD in the ectopic group and the incumbent group. There are obvious differences in the expression of CD5 and apoptosis inhibitory protein XIAP, the obvious downregulation of PDCD5 expression and the obvious up-regulation of the expression of XIAP, suggesting that the abnormal expression of PDCD5 and XIAP may be one of the important reasons for the abnormal phenomenon of apoptosis in endometrium cells of AM patients. It is one of the important reasons for participating in AM hair disease and ultimately contributing to the formation of AM.3, P. The expression of DCD5 and XIAP in ectopic and eutopic endometrium was negatively correlated, suggesting that there were obvious antagonism or negative feedback regulation between the two groups. In addition, the expression of PDCD5 was the lowest in the three group of endometrium tissues, and the expression of XIAP was the highest. This further indicated that the mutual antagonism or negative feedback regulation of PDCD5 and XIAP was ectopic and negative feedback. The expression of endometrium in endometrium is more obvious, suggesting that the imbalance in the expression of two of the endometrium cells may be one of the key factors that can further promote the development of AM by the ectopic endometrium cells that can adapt to the ectopic environment and grow in it.
【學位授予單位】：瀘州醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R711.71
【參考文獻】
相關期刊論文 前10條
1 劉朝暉,張岱,李克敏,廖秦平;PDCD5蛋白在正常宮頸、宮頸上皮內(nèi)瘤樣病變和宮頸癌中的表達[J];北京大學學報(醫(yī)學版);2004年04期
2 譚萬龍;熊林;鄭少斌;郁兆存;齊桓;杜躍軍;吳們;;腎透明細胞癌PDCD5表達及與預后的關系[J];南方醫(yī)科大學學報;2006年09期
3 馮靜,崔恒,魏麗惠,馬大龍;TFAR19蛋白在卵巢上皮性癌中的表達[J];中國婦產(chǎn)科臨床;2002年03期
4 盧北燕;孫瑞擎;郭昕;;PDCD5和Survivin在人宮頸癌中的表達和臨床意義[J];黑龍江醫(yī)藥科學;2010年05期
5 李書君;于靖;趙雪飛;姜穎;劉紫君;于曉光;;凋亡相關基因PDCD5對前列腺癌細胞PC-3M-1E8促凋亡作用的研究[J];中華男科學雜志;2007年11期
6 張麗;石彬;任秀朋;趙昕;劉效群;;XIAP、XAF1、TNF-α在子宮內(nèi)膜異位癥發(fā)病機制中的作用[J];中國計劃生育學雜志;2012年09期
7 趙杰;張秀軍;趙靜;趙麗娜;劉鵬;;PDCD5基因及其與疾病的關系[J];生命的化學;2006年02期
8 劉志英;程華;肖剛;;乳腺癌組織中ER、CerbB-2、XIAP的表達變化及意義[J];山東醫(yī)藥;2012年46期
9 李紅霞,冷靜,姚玉宇,李風山,彭韜,倉為民,錢寧;子宮內(nèi)膜異位癥與細胞凋亡關系的研究[J];江蘇醫(yī)藥;2000年11期
10 劉靜;蔣常文;;X連鎖凋亡抑制蛋白XIAP腫瘤相關性研究現(xiàn)狀[J];醫(yī)學綜述;2009年19期
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