本文選題：三氯生 + 秀麗線蟲��； 參考：《新鄉(xiāng)醫(yī)學(xué)院》2014年碩士論文

【摘要】：背景：內(nèi)分泌干擾物(endocrine disrupting chemicals,EDCs),如三氯生(Triclosan,TCS)通過與激素受體結(jié)合或影響細(xì)胞信號途徑等方式,模擬或部分模擬激素,發(fā)揮類激素樣或抗激素樣作用,干擾機(jī)體內(nèi)激素的合成、分泌、轉(zhuǎn)運(yùn)、結(jié)合、代謝及調(diào)節(jié)等過程,從而影響兒童的生長發(fā)育。 秀麗線蟲作為一種模式動物,具有生命周期短,繁殖速度快,個體小,容易培養(yǎng)且遺傳與發(fā)育背景清楚等諸多優(yōu)點(diǎn),已廣泛應(yīng)用于生物學(xué)各領(lǐng)域的研究。秀麗線蟲對多種藥物、污染物敏感,且行為反應(yīng)模式穩(wěn)定、結(jié)果可靠,已被用于藥物篩選、檢測和評價污染。 目的：觀察三氯生(Triclosan,TCS)對秀麗線蟲(Caenorhabditis elegans,c.elegans)子代(F1)的運(yùn)動能力、后代數(shù)目及世代時間、生長發(fā)育等生物學(xué)指標(biāo)的影響,為研究TCS毒性效應(yīng)及其機(jī)制提供理論依據(jù)。 方法：實驗設(shè)置對照組和4個三氯生暴露組,濃度分別為100nmol/L、1μmol/L、10μmol/L、20μmol/L,從c.elegans L4期開始暴露,暴露時間24h,暴露后轉(zhuǎn)移至無TCS培養(yǎng)板繼續(xù)培養(yǎng)。觀察TCS對c.elegans子代(F1)頭部擺動頻率、身體彎曲頻率、后代數(shù)目及世代時間、體長及體寬等生物學(xué)指標(biāo)的影響；采用基因芯片技術(shù)分析對照組和TCS20μmol/L差異表達(dá)基因及主要作用。各組數(shù)據(jù)以均數(shù)±標(biāo)準(zhǔn)差(x±s)表示,應(yīng)用SPSS20.0軟件包進(jìn)行統(tǒng)計學(xué)分析,多組間均數(shù)采用單因素方差分析(ANOVA),以P0.05作為顯著性檢驗標(biāo)準(zhǔn)。 結(jié)果：1.各濃度TCS水平組c.elegans Fl頭部擺動頻率較對照組降低(P0.01),ANOVA示,三氯生暴露后,c.elegans Fl頭部擺動能力受到顯著影響(F=卯.245,P0.01)；各濃度TCS水平組c.elegans F1身體彎曲頻率較對照組降低(P0.01),ANOVA示,三氯生暴露后,c.elegans F1身體彎曲能力受到顯著影響(F=25.636,P0.01)。 2.與對照組比,暴露在100mol/L、10μmol/L、20μmol/L三氯生中的c.elegans F1個體的后代數(shù)目分別降低了4.71%、25.60%、39.45%、58.67%, AN OVA結(jié)果示,三氯生暴露后對c.elegans生殖能力產(chǎn)生極顯著影響(F=461.593,P0.01)；與對照組相比,三氯生暴露后秀麗線蟲的世代時間縮短了2.14%--5.38%(P0.01),并且表現(xiàn)出濃度依賴性表型。ANOVA結(jié)果示、三氯生暴對c.elegans F1世代時間產(chǎn)生顯著影響(F=27520,P0.01)。 3.與對照組比,暴露在100nmol/L、1μmol/L、101μmol/L、20μmo1/L三氯生中的c.elegans F1L4期幼蟲體長及體寬變化不明顯(P0.05),無統(tǒng)計學(xué)意義。 4.基因芯片結(jié)果示：對照組和TCS20μmol/L暴露后組間差異大于2倍,組內(nèi)差異小的表達(dá)差異基因共有113條,其中大部分為與線蟲神經(jīng)發(fā)育和調(diào)節(jié)運(yùn)動相關(guān)的基因；挑選組間差異大于3倍,組內(nèi)差異小的7個基因進(jìn)行驗證,驗證結(jié)果與基因芯片相符。 結(jié)論：1.孕期三氯生暴露對秀麗線蟲的子代運(yùn)動、生殖影響顯著,提示三氯生存在生殖毒性及抑制秀麗線蟲神經(jīng)發(fā)育。 2.GO分析和Pathway分析的結(jié)果說明孕期暴露三氯生后可能是因為基因的差異表達(dá)對線蟲的運(yùn)動及生殖能力產(chǎn)生影響。 3.基因芯片結(jié)果示差異表達(dá)基因較多,由此推測孕期三氯生暴露可能會對秀麗線蟲子代產(chǎn)生更廣泛的潛在影響。
[Abstract]:Background: endocrine disrupting chemicals (EDCs), such as three chloroplastic (Triclosan, TCS), which mimic or partially mimic hormone like or antisteroid effects by combining with hormone receptors or affecting cell signaling pathways, and interferes with the synthesis, secretion, transport, binding, metabolism, and regulation of hormones in the body. It affects the growth and development of children.
As a model animal, Caenorhabditis elegans has many advantages, such as short life cycle, fast propagation speed, small individual, easy cultivation and clear genetic background, and so on. It has been widely used in various fields of biology. The elegans are sensitive to a variety of drugs and contaminants, and are stable in reaction mode and reliable. Detection and evaluation of pollution.
Objective: To observe the effects of Triclosan (Triclosan (TCS) on the biological indexes of Caenorhabditis elegans (C.elegans) progeny (F1), the number of offspring and generation time, and the growth and development of the offspring (F1), and provide a theoretical basis for the study of the toxicity and mechanism of TCS.
Methods: the experiment set up the control group and 4 three chlorobi exposure groups, the concentration was 100nmol/L, 1 mu mol/L, 10 mu mol/L, 20 mu mol/L, from C.elegans L4 stage to exposure, exposure time 24h, and then transferred to no TCS culture plate to continue culture. The frequency of TCS to C.elegans sub generation (F1), body bending frequency, the number of offspring and generation time were observed. The effect of body length and body width and other biological indexes, the gene chip technique was used to analyze the differentially expressed genes and the main functions of the control group and TCS20 mu mol/L. The data of each group were expressed by the mean number of standard deviation (x + s), and the SPSS20.0 software package was used for statistical analysis. The average number of groups was analyzed by single factor variance analysis (ANOVA), and P0.05 was used as the significance. Test standard.
Results: 1. the head oscillating frequency of C.elegans Fl in the TCS level group was lower than that of the control group (P0.01). ANOVA showed that the head swing ability of C.elegans Fl was significantly affected after three chlorinated exposure (F= sockets.245, P0.01), and the flexural frequency of the body was lower than that of the control group. F1 body flexion ability was significantly affected (F=25.636, P0.01).
2. compared with the control group, the number of descendants of C.elegans F1 individuals exposed to 100mol/L, 10, mol/L, 20 mol/L three chloride decreased by 4.71%, 25.60%, 39.45%, 58.67%, and AN OVA results showed that three chloride exposure had a significant effect on C.elegans reproduction (F=461.593, P0.01); compared with the control group, three chloro was exposed to Caenorhabditis elegans. The generation time shortened 2.14%--5.38% (P0.01) and showed a concentration dependent phenotypic.ANOVA result indicating that three chlorochlorine had a significant effect on the C.elegans F1 generation time (F=27520, P0.01).
3. compared with the control group, the changes of body length and body width of C.elegans F1L4 larvae in 100nmol/L, 1, 101, 101, and 20 mo1/L three chloride were not significant (P0.05). There was no statistical significance.
The results of 4. gene chip showed that the difference between the control group and the TCS20 mu mol/L was more than 2 times, and there were 113 differentially differentially expressed genes in the group. Most of them were genes related to the development of the nematode nerve and the regulation of the movement. The differences between the selected groups were more than 3 times, and 7 genes with small differences in the group were verified, and the results were verified with the gene core. The film coincide.
Conclusion: during the 1. trimester, three chlorinone exposure had a significant effect on the reproduction of the offspring of the nematode Caenorhabditis elegans, suggesting that three chloro had reproductive toxicity and inhibited the development of the Caenorhabditis elegans.
The results of 2.GO analysis and Pathway analysis showed that exposure to three chlorprospan during pregnancy may be due to differential expression of genes affecting the movement and reproductive capacity of nematodes.
3. gene chip results showed that there were more differentially expressed genes. It is speculated that exposure to three chloro chloride during pregnancy may have a wider potential impact on the offspring of the male worm.
【學(xué)位授予單位】：新鄉(xiāng)醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R715.3

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