本文選題：子宮內(nèi)膜容受性 + FKBP51��； 參考：《山東大學(xué)》2014年碩士論文

【摘要】：[目的]容受性較好的子宮內(nèi)膜和發(fā)育正常的胚泡,是保證妊娠過程順利進(jìn)行的兩個決定性因素。排卵正常的不孕患者最常見的妊娠失敗原因就是子宮內(nèi)膜容受性的降低導(dǎo)致的植入失敗。在育齡女性多種疾病的發(fā)展進(jìn)程中,都存在著各種各樣導(dǎo)致子宮內(nèi)膜容受性降低的因素,這些因素相互影響、相互作用最終導(dǎo)致了這些患者妊娠率的低下。弄清此過程中降低子宮內(nèi)膜容受性的因素的作用機(jī)制,對指導(dǎo)臨床采取干預(yù)措施提高患者的生育能力,增加妊娠率有著至關(guān)重要的意義。目前,大量研究關(guān)注于親免素蛋白FKBP51,該蛋白在性激素的調(diào)節(jié)過程的許多方面都起到一定的作用,其表達(dá)受到多種性激素水平的調(diào)控,并且還作為性激素受體復(fù)合物的組成部分,調(diào)節(jié)性激素在各組織的作用水平。本研究旨在探索：伴隨子宮內(nèi)膜增殖期向分泌期的轉(zhuǎn)換,其性激素水平變化是否會導(dǎo)致FKBP51蛋白在子宮內(nèi)膜的表達(dá)水平產(chǎn)生明顯的差異。 [方法]收集80例子宮內(nèi)膜活檢的內(nèi)膜標(biāo)本,制作子宮內(nèi)膜組織組合芯片。對芯片上每一點(diǎn)標(biāo)本進(jìn)行病理分期,用免疫組化的方法檢測FKBP51抗體在子宮內(nèi)膜組織組合芯片(UTN801)上的表達(dá)。顯微鏡觀察半定量評定FKBP51表達(dá)水平,使用灰度分析軟件統(tǒng)計(jì)灰度值,采用SPSS v19.0中兩獨(dú)立樣本T檢驗(yàn)對陽性積分和灰度值進(jìn)行統(tǒng)計(jì)學(xué)分析。 [結(jié)果]隨性激素調(diào)節(jié)水平的變化,FKBP51在子宮內(nèi)膜組織的表達(dá)水平存在顯著差異,子宮內(nèi)膜分泌期的FKBP51表達(dá)水平明顯高于增殖期表達(dá)水平(P0.001)。 [結(jié)論]在子宮內(nèi)膜組織,伴隨性激素水平的變化,FKBP51的表達(dá)水平有顯著的差異。正常子宮內(nèi)膜周期中,增殖期向分泌期的轉(zhuǎn)變,伴隨著雌激素和雄激素水平作用的下調(diào),孕激素作用水平的上調(diào),在此過程中,FKBP51的表達(dá)水平隨之升高,分泌期其表達(dá)水平顯著高于增殖期。
[Abstract]:Objective: endometrium with good receptivity and blastocyst with normal development are two decisive factors to ensure the smooth progress of pregnancy. The most common cause of pregnancy failure in infertile patients with normal ovulation is the failure of implantation due to reduced endometrial receptivity. In the development process of many diseases of women of childbearing age, there are various factors that lead to the decrease of endometrial receptivity. These factors affect each other and ultimately lead to the low pregnancy rate of these patients. Understanding the mechanism of reducing endometrial receptivity in this process is of great significance in guiding clinical intervention to improve fertility and increase pregnancy rate. At present, a large number of studies have focused on the protein FKBP51, which plays a role in many aspects of the regulation of sex hormones, and its expression is regulated by various levels of sex hormones, and is also a component of the sex hormone receptor complex. To regulate the action of sex hormones in various tissues. The aim of this study was to explore whether the changes of sex hormone levels in endometrium may lead to significant differences in the expression of FKBP51 protein in endometrium with the transition from endometrial proliferation to secretory phase. Methods 80 endometrial specimens from endometrial biopsy were collected and microarray of endometrial tissue was made. The expression of FKBP51 antibody on UTN801 was detected by immunohistochemistry. The expression level of FKBP51 was evaluated by microscope. The gray value of FKBP51 was calculated by gray analysis software, and the positive score and gray value were analyzed statistically by T test of two independent samples in SPSS v19.0. [results] there was significant difference in the expression of FKBP51 in endometrial tissues. The expression level of FKBP51 in secretory phase of endometrium was significantly higher than that in proliferative phase (P0.001). [conclusion] there is significant difference in the expression of FKBP51 in endometrium with the change of sex hormone level. During the normal endometrial cycle, the transition from proliferative phase to secretory phase, with the down-regulation of estrogen and androgen levels, and the up-regulation of progesterone action, the expression of FKBP51 increased during the normal endometrial cycle. The expression level in secretory phase was significantly higher than that in proliferative stage.
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R711.6

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 王瑤;馮云;牛志宏;黃曉燕;張愛軍;;子宮內(nèi)膜輕創(chuàng)對著床相關(guān)因子及IVF妊娠結(jié)局影響的研究[J];生殖與避孕;2007年07期

，

本文編號：2054162