發(fā)布時(shí)間：2018-06-18 17:44

  本文選題：子宮內(nèi)膜異位癥 + 遷移能力��； 參考：《中南大學(xué)》2014年碩士論文

【摘要】：目的 本課題通過(guò)原代培養(yǎng)正常人及子宮內(nèi)膜異位癥患者子宮內(nèi)膜細(xì)胞,比較不同細(xì)胞的形態(tài)學(xué)結(jié)構(gòu)及遷移能力的差別。通過(guò)給予不同細(xì)胞雌、孕激素及原礬酸鈉(SoV)干預(yù),測(cè)定肝再生磷酸酶3(phosphatase of regenerating liver-3, PRL-3)的表達(dá)情況及變化,探討PRL-3與子宮內(nèi)膜細(xì)胞遷徙能力差異的關(guān)系,研究子宮內(nèi)膜異位癥高轉(zhuǎn)移高侵襲的發(fā)病機(jī)制。 方法 1.原代培養(yǎng)正常人及子宮內(nèi)膜異位癥患者子宮內(nèi)膜細(xì)胞,比較不同細(xì)胞的形態(tài)學(xué)結(jié)構(gòu)。 2.用劃線實(shí)驗(yàn)比較不同細(xì)胞的遷移能力差別。 3. qT-PCR法及Western Blot法測(cè)定不同細(xì)胞中PRL-3的表達(dá)。 4.予以PRL-3抑制劑原礬酸鈉、雌、孕激素干預(yù)后,行劃線實(shí)驗(yàn)確定細(xì)胞遷移能力變化。qT-PCR法及Western Blot法測(cè)定不同細(xì)胞中PRL-3的表達(dá)。 結(jié)果 1.子宮內(nèi)膜細(xì)胞由腺上皮細(xì)胞及間質(zhì)細(xì)胞組成,腺上皮細(xì)胞角蛋白免疫組化染色呈陽(yáng)性反應(yīng),間質(zhì)細(xì)胞波形蛋白免疫組化染色呈陽(yáng)性反應(yīng)。隨著傳代次數(shù)增加,角蛋白陽(yáng)性細(xì)胞數(shù)量越來(lái)越少,波形蛋白陽(yáng)性細(xì)胞所占比例占絕大部分,為主要細(xì)胞。 2.劃線實(shí)驗(yàn)結(jié)果顯示：在位內(nèi)膜細(xì)胞在36小時(shí)后細(xì)胞遷移的距離大于正常子宮內(nèi)膜細(xì)胞遷移的距離；對(duì)照組內(nèi)膜細(xì)胞遷移的距離大于加入0.5umol/l SoV組細(xì)胞遷移的距離；10-6mol/l雌二醇組細(xì)胞遷移的距離大于對(duì)照組細(xì)胞遷移的距離。對(duì)照組內(nèi)膜細(xì)胞遷移的距離大于10-5mol/l孕酮細(xì)胞遷移的距離。 3.PRL-3在EMs患者在位內(nèi)膜上皮細(xì)胞中表達(dá)強(qiáng)于正常人細(xì)胞中的表達(dá),兩者之間差異有統(tǒng)計(jì)學(xué)意義(P0.05)；對(duì)照組細(xì)胞中PRL-3表達(dá)強(qiáng)于加入SoV組細(xì)胞中表達(dá),兩者之間差異有統(tǒng)計(jì)學(xué)意義(P0.05)；加入雌激素組細(xì)胞中PRL-3表達(dá)強(qiáng)于對(duì)照組細(xì)胞中表達(dá),兩者之間差異有統(tǒng)計(jì)學(xué)意義(P0.05)；對(duì)照組細(xì)胞中PRL-3表達(dá)強(qiáng)于加入孕激素組細(xì)胞中的表達(dá),兩者之間差異有統(tǒng)計(jì)學(xué)意義(P0.05) 結(jié)論 1.EMs患者在位子宮內(nèi)膜細(xì)胞遷移能力強(qiáng)于正常人子宮內(nèi)膜細(xì)胞遷移能力,PRL-3在EMs在位子宮內(nèi)膜細(xì)胞中表達(dá)強(qiáng)于正常人子宮內(nèi)膜細(xì)胞,子宮內(nèi)膜細(xì)胞可能通過(guò)PRL-3促進(jìn)子宮內(nèi)膜細(xì)胞的遷移能力。 2.雌激素可能通過(guò)上調(diào)PRL-3表達(dá),正性調(diào)節(jié)子宮內(nèi)膜細(xì)胞的遷移。孕激素可能通過(guò)下調(diào)PRL-3表達(dá),負(fù)性調(diào)節(jié)子宮內(nèi)膜細(xì)胞的遷移。 3.SoV能通過(guò)抑制PRL-3的表達(dá),抑制子宮內(nèi)膜細(xì)胞的遷移能力。
[Abstract]:Purpose

In order to study the relationship between PRL - 3 and migration ability of endometrial cells , the relationship between PRL - 3 and migration ability of endometrial cells was determined by the intervention of estrogen , progesterone and sodium sulfate ( SoV ) in different cells .

method

1 . The endometrial cells of normal and endometriosis were cultured in primary culture , and the morphological structure of different cells was compared .

2 . The difference of migration ability of different cells was compared by scoring experiment .

3 . The expression of PRL - 3 in different cells was determined by qT - PCR and Western Blot .

4 . The expression of PRL - 3 in different cells was determined by the method of qT - PCR and Western Blot .

Results

1 . The endometrial cells were composed of glandular epithelial cells and stroma cells . The cytokeratins of the glandular epithelial cells showed a positive reaction with immunohistochemical staining . The number of keratin - positive cells decreased with the increase of the number of passages , and the percentage of the positive cells of the protein - positive cells accounted for most of the cells , which were the main cells .

2 . The results showed that the migration distance of the cells after 36 hours was greater than that of normal endometrial cells .
The migration of endometrial cells in the control group was greater than that in the group of 0.5mol / l SoV group .
The distance of migration of cells from 10 - 6 mol / l estradiol group was greater than that of control group .

3 . The expression of PRL - 3 in the epithelial cells of endometriosis was significantly higher than that in normal human cells ( P0.05 ) .
The expression of PRL - 3 in the control group was stronger than that in control group ( P0.05 ) .
PRL - 3 expression in estrogen group was stronger than that in control group ( P0.05 ) .
The expression of PRL - 3 in the control group was stronger than that in control group ( P0.05 ) .

Conclusion

1 . In endometriosis , the migration ability of endometrium cells was stronger than that of normal controls , PRL - 3 expression was stronger than normal endometrium cells in endometriosis , and the migration ability of endometrial cells could be promoted by PRL - 3 .

2 . Estrogen may regulate the migration of endometrial cells by up - regulating the expression of PRL - 3 , and progesterone may regulate the migration of endometrial cells by downregulating PRL - 3 expression .

3 . SoV can inhibit the migration of endometrial cells by inhibiting the expression of PRL - 3 .
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R711.71

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相關(guān)期刊論文 前2條

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本文編號(hào)：2036346