本文選題：蛋白激酶CK2α + β-Catenin��； 參考：《遼寧醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的 通過研究蛋白激酶CK2α在卵巢癌組織和OVCAR3細胞株中的表達情況，分析CK2α與β-Catenin、Survivin在卵巢癌中的相互作用關(guān)系，進一步探討蛋白激酶CK2α在卵巢癌發(fā)生發(fā)展中的作用機制。 方法 1、采用免疫組化SP法檢測CK2α、β-Catenin、Survivin在8例卵巢單純性囊腫、8例卵巢良性囊腺瘤、20例卵巢漿液性囊腺癌，9例卵巢粘液性囊腺癌組織標本中的表達情況，并分析CK2α、β-Catenin、Survivin在卵巢癌中的相關(guān)性。 2、體外培養(yǎng)人卵巢癌OVCAR3細胞株，實驗分為空白對照組（未處理組）、DMSO溶劑組、TBB組（TBB終濃度分別為12.5μmol/L、25μmol/L、50μmol/L、75μmol/L、100μmol/L）。藥物作用24h后，MTT比色法測定細胞生長抑制率。 3、應(yīng)用RT-PCR和Western blotting技術(shù)分別檢測蛋白激酶CK2α、β-Catenin、Survivin mRNA和蛋白在各組人卵巢癌OVCAR3細胞中的表達，分析各組CK2α和β-Catenin、Survivin基因表達的變化及它們之間的相互關(guān)系。 結(jié)果 1、與卵巢單純性囊腫和良性卵巢腫瘤組織相比，CK2α、β-Catenin、Survivin在卵巢癌中的蛋白表達水平明顯提高P0.05㖞；CK2α與β-Catenin、Survivin在卵巢癌中表達呈正相關(guān)（R=0.438、0.479，P0.05）；CK2α、β-Catenin、Survivin在低 中分化組的蛋白表達水平陽性率明顯高于高分化組(P0.05)；CK2α、β-Catenin、Survivin在Ⅱ Ⅲ期的蛋白表達水平陽性率與Ⅰ期相比明顯增加，差異有統(tǒng)計學(xué)意義(P0.05)。 2、與DMSO溶劑組相比，以不同濃度TBB組處理的OVCAR3細胞生長抑制率隨TBB濃度的增加而明顯增加，呈劑量依賴性。 3、RT-PCR結(jié)果表明，隨著TBB濃度的增加，OVCAR3細胞株CK2α的mRNA表達水平降低，但β-Catenin、Survivin的mRNA表達水平并沒有明顯變化。 4、Western blotting結(jié)果表明，與對照組相比，加入TBB后，CK2α的蛋白表達受到高效特異的抑制，β-Catenin、Survivin蛋白表達水平明顯降低。 結(jié)論 1、蛋白激酶CK2α、β-Catenin、Survivin高表達可能與卵巢癌的發(fā)生和發(fā)展有關(guān),并且CK2α與β-Catenin、Survivin在卵巢癌中的表達呈正相關(guān) 2、TBB對人卵巢癌OVCAR3細胞株細胞生長增殖具有顯著抑制作用，其機制可能與降低CK2α基因表達，進而下調(diào)細胞增殖、凋亡相關(guān)基因β-Catenin、Survivin蛋白表達水平與活性有關(guān)。
[Abstract]:Objective to study the expression of protein kinase CK2 偽 in ovarian carcinoma and OVCAR3 cell line, and to analyze the interaction between CK2 偽 and 尾 -Catenin survivin in ovarian cancer, and to explore the mechanism of protein kinase CK2 偽 in the development of ovarian cancer. Methods 1. Immunohistochemical SP method was used to detect the expression of CK2 偽, 尾 -Catenin survivin in 8 cases of ovarian simple cysts and 8 cases of benign ovarian cystadenoma, 20 cases of ovarian serous cystadenocarcinoma and 9 cases of ovarian mucinous cystadenocarcinoma. The correlation of CK2 偽, 尾 -Catenin survivin in ovarian cancer was analyzed. 2Human ovarian cancer OVCAR3 cell line was cultured in vitro and divided into blank control group (the final concentration of TBB in untreated group was 12.5 渭 mol / L ~ 25 渭 mol / L ~ 50 渭 mol / L ~ (75) 渭 mol 路L ~ (-1) ~ 100 渭 mol 路L ~ (-1). The cell growth inhibition rate was measured by MTT colorimetry 24 hours after treatment. The expression of protein kinase CK2 偽, 尾 -Catenin survivin mRNA and protein in human ovarian cancer OVCAR3 cells was detected by RT-PCR and Western blotting. The expression changes of CK2 偽 and 尾 -Catenin survivin gene in each group and their relationship were analyzed. Results (1) compared with simple ovarian cyst and benign ovarian tumor, the expression of CK2 偽, 尾 -Catenin survivin in ovarian carcinoma was significantly higher than that in ovarian carcinoma. The expression of P0.05a and 尾 -Catenin survivin in ovarian carcinoma was positively correlated with the expression of 尾 -Catenin survivin in ovarian carcinoma. The positive rate of CK2 偽, 尾 -Catenin survivin protein expression in poorly differentiated group was significantly higher than that in well-differentiated group (P 0.05). The positive rate of 尾 -Catenin survivin protein expression in stage 鈪，

本文編號：2027621