本文選題：宮頸癌 + 宮頸上皮內(nèi)瘤變��； 參考：《安徽醫(yī)科大學(xué)》2014年碩士論文

【摘要】：在婦科最常見(jiàn)的惡性腫瘤中，宮頸癌是其中之一，全世界每年宮頸癌新增病例約529800，死亡病例約275100，其中發(fā)展中國(guó)家每年新增約453300，死亡病例約242000，，占全世界的85%以上[1]。宮頸上皮內(nèi)瘤變（cervical intraepizhelialneoplasia，CIN）是與子宮頸浸潤(rùn)癌密切相關(guān)的一組子宮頸病變，具有兩種不同結(jié)局：一是病變自然消退；二是病變發(fā)展，進(jìn)展為浸潤(rùn)癌[2]。Trop-2由定位于1P32上的TACSTD2（腫瘤相關(guān)鈣信號(hào)轉(zhuǎn)導(dǎo)基因2）編碼[4]、由323個(gè)氨基酸構(gòu)成的的單次跨膜糖蛋白[3]，為一類鈣離子通道信號(hào)轉(zhuǎn)化器，但其信號(hào)轉(zhuǎn)導(dǎo)的具體機(jī)制仍不清楚[5]。目前國(guó)內(nèi)尚未見(jiàn)Trop-2與宮頸病變關(guān)系的臨床研究報(bào)道，本研究用RT-PCR、免疫組織化學(xué)等技術(shù)檢測(cè)Trop-2在宮頸病變中的表達(dá)，探討其與宮頸病變臨床病理的關(guān)系。 目的觀察并探討Trop-2在人宮頸病變中的表達(dá)及其臨床相關(guān)性。方法運(yùn)用RT-PCR的方法檢測(cè)6例正常宮頸組織、6例CIN Ⅰ或CIN Ⅱ組織、6例CINⅢ組織和6例宮頸癌組織中Trop-2mRNA的表達(dá)情況；免疫組織化學(xué)方法檢測(cè)Trop-2蛋白在20例正常宮頸組織、20例CIN Ⅰ或CIN Ⅱ組織、20例CINⅢ組織及20例宮頸癌組織中的表達(dá)情況。 結(jié)果1、Trop-2mRNA在上述的宮頸病變組織中均有表達(dá)，且隨著病變程度的加重，表達(dá)量越多，Trop-2mRNA表達(dá)水平隨著宮頸病變程度的逐步加深而依次升高。正常宮頸組、CINⅠCINⅡ組、CINⅢ組、宮頸癌組中Trop-2mRNA的平均灰度值分別為0.52,0.76,1.00,1.55，各組之間差異均具有統(tǒng)計(jì)學(xué)意義(P0．01)。2、Trop-2蛋白在CIN組織及宮頸癌組織中呈陽(yáng)性表達(dá)，主要定位在細(xì)胞膜上，且表達(dá)水平隨著宮頸病變程度的加重而依次升高（P0.01）。在低度上皮內(nèi)瘤變組織中，表皮基底層以上三分之一細(xì)胞可見(jiàn)連續(xù)性淺棕色著色，高度上皮內(nèi)瘤變組織表皮基底層三分之二及上皮全層亦見(jiàn)連續(xù)性淺棕色著色，而在正常宮頸組織上皮未見(jiàn)淺棕色著色，個(gè)別組織呈現(xiàn)黃色，但并不連續(xù)；3、宮頸癌組中，Trop-2表達(dá)有19例，強(qiáng)陽(yáng)性表達(dá)有15例，陽(yáng)性表達(dá)3例，弱陽(yáng)性表達(dá)1例，陰性表達(dá)1例；在CINⅠ和CINⅡ組中，呈陽(yáng)性表達(dá)的共有16例，其中強(qiáng)陽(yáng)性表達(dá)2例，陽(yáng)性表達(dá)4例，弱陽(yáng)性表達(dá)10；在CINⅢ組中，呈陽(yáng)性表達(dá)的總有18例，其中強(qiáng)陽(yáng)性表達(dá)3例，陽(yáng)性表達(dá)12例，弱陽(yáng)性表達(dá)3例，陰性表達(dá)2例。而在正常宮頸組織中，Trop-2弱陽(yáng)性表達(dá)2例，陰性表達(dá)18例，隨著宮頸病變程度的加深，呈陽(yáng)性或強(qiáng)陽(yáng)性表達(dá)的例數(shù)越多。 結(jié)論1、Trop-2的高表達(dá)可能參與宮頸病變的發(fā)生發(fā)展；2、Trop-2或可作為宮頸癌臨床篩查及診斷的一個(gè)新的標(biāo)記物3、Trop-2有望成為靶向治療宮頸癌的一個(gè)靶點(diǎn)。
[Abstract]:Among the most common gynecologic malignancies, cervical cancer is one of them. About 529800 of the new cases of cervical cancer are newly added in the world, about 275100 of the deaths in the world, of which about 453300 of the developing countries are added each year, and about 242000 of the deaths, accounting for more than 85% [1]. cervical intraepithelial neoplasia (cervical intraepizhelialneoplasia, CIN) from the cervix of the world. A group of cervical lesions closely related to invasive cancer has two different outcomes: one is the natural degeneration of the lesion, and the two is the development of the lesion, and the progression is that the [2].Trop-2 of the invasive cancer is encoded by the TACSTD2 (tumor related calcium signal transduction gene 2) located on the 1P32, and the single transmembrane glycoprotein [3], which is constructed by 323 amino acids, is a class of calcium ions. The specific mechanism of signal transduction, however, is still not clear in the specific mechanism of signal transduction. There is no clinical study on the relationship between Trop-2 and cervical lesions at present in [5].. This study uses RT-PCR, immunohistochemistry and other techniques to detect the expression of Trop-2 in cervical lesions, and to explore the relationship with the clinicopathology of cervical disease.
Objective To observe and explore the expression and clinical relevance of Trop-2 in human cervical lesions. Methods RT-PCR was used to detect the expression of Trop-2mRNA in 6 normal cervical tissues, 6 CIN I or CIN II tissues, 6 CIN III tissues and 6 cervical cancer tissues, and 20 normal cervical groups were detected by immunohistochemical method. Weave 20 cases of CIN I or CIN II tissue, 20 cases of CIN III and 20 cases of cervical cancer.
Results 1, Trop-2mRNA was expressed in all the cervical lesions, and the more the expression was increased with the severity of the lesion, the level of Trop-2mRNA expression increased with the gradual deepening of the degree of cervical lesions. The average gray value of Trop-2mRNA in the normal cervix group, the CIN I CIN II group, the CIN III group and the cervical cancer group was 0.52,0.76,1.00,1., respectively. 55, the difference between each group was statistically significant (P0.01).2, Trop-2 protein was positive in the CIN tissue and cervical cancer tissue, mainly located on the cell membrane, and the expression level increased in turn (P0.01) with the aggravation of the degree of cervical lesions. In the low degree intraepithelial neoplasia, the epidermis above the basal layer of the 1/3 cells can be seen. Continuous light brown coloring, 2/3 of the epidermis basal layer of the high intraepithelial neoplasia and the epithelia also showed continuous light brown color, but no light brown color was found in the normal cervical tissue epithelium, and some tissues were yellow, but not continuous. 3, 19 cases of Trop-2 were expressed in the cervical cancer group, 15 cases were strongly positive and 3 cases were positive. There were 1 cases of weak positive expression and negative expression in 1 cases. There were 16 positive expressions in group CIN I and CIN II, of which 2 cases were strongly positive, 4 cases were positive and 10 were weakly positive. There were 18 positive expressions in group CIN III, of which 3 were strongly positive, 12 was positive, 3 cases were positive, 3 negative expression, and 2 cases negative. In the cervical tissue, Trop-2 was weakly positive in 2 cases and negative in 18 cases. With the degree of cervical lesions increasing, the number of positive or strong positive cases increased.
Conclusion 1, high expression of Trop-2 may be involved in the development of cervical lesions, and 2, Trop-2 may serve as a new marker for the clinical screening and diagnosis of cervical cancer, and Trop-2 is expected to be a target for targeted treatment of cervical cancer.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.33

【共引文獻(xiàn)】

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本文編號(hào)：2016162