本文選題：VEGF + MMP-2�。� 參考：《南華大學(xué)》2014年碩士論文

【摘要】：目的： 檢測血管內(nèi)皮生長因子(vaseularendothelialgrowthfaetor,VEGF)、基質(zhì)金屬蛋白酶2(matrix metalloproteinase2, MMP-2)和基質(zhì)金屬蛋白酶9(matrixmetalloproteinase9, MMP-9)在子宮內(nèi)膜息肉組織（復(fù)雜型增生及簡單型增生）、息肉旁內(nèi)膜組織及子宮正常內(nèi)膜組織中的蛋白表達(dá)；探討VEGF、MMP-2及MMP-9在子宮內(nèi)膜息肉發(fā)病機(jī)制中的作用及其相關(guān)性。 方法： 標(biāo)本收集：收集湘南學(xué)院附屬醫(yī)院2009年12月到2012年12月60例確診為子宮內(nèi)膜息肉組織樣本為觀察組，其中經(jīng)確診有30例為復(fù)雜性增生型子宮內(nèi)膜息肉組織樣本，30例為簡單增生型子宮內(nèi)膜息肉組織樣本。然后再選擇同期子宮內(nèi)膜息肉旁的內(nèi)膜組織樣本30例為對照1組，選擇正常子宮內(nèi)膜組織樣本30例為對照2組。 免疫組化：取60例子宮內(nèi)膜息肉、30例子宮內(nèi)膜息肉旁的內(nèi)膜組織及30例正常子宮內(nèi)膜的組織石蠟標(biāo)本，免疫組織化學(xué)染色，顯微鏡下觀察VEGF、MMP-2及MMP-9蛋白表達(dá)。 結(jié)果判斷：根據(jù)染色細(xì)胞進(jìn)行定性判斷；采用陽性細(xì)胞百分率的半定量法來判定染色結(jié)果，在每例的染色結(jié)果中，陽性程度的判定采用積分方法：0~2分為陰性(-)；3~5分為弱陽性(+)；6~8分為陽性(++)；9~12分為強(qiáng)陽性(+++)。所有結(jié)果均由兩位病理醫(yī)師獨(dú)立評定。 統(tǒng)計分析：采用SPSS19.0統(tǒng)計軟件包進(jìn)行統(tǒng)計學(xué)分析。根據(jù)資料性質(zhì)采用不同統(tǒng)計方法：兩樣本率的比較采用x2檢驗(yàn)或fisher確切概率法；等級資料的比較采Mann-Whitney秩和檢驗(yàn)。相關(guān)性分析采用spearman相關(guān)分析；計量資料采用t檢驗(yàn)及方差分析。P0.05為統(tǒng)計學(xué)顯著性意義標(biāo)準(zhǔn)。 結(jié)果： （1）與對照1組和對照2組相比，子宮內(nèi)膜息肉組包括復(fù)雜型增生息肉組和簡單增生型息肉組VEGF蛋白表達(dá)增高，差異有統(tǒng)計學(xué)意義(P0.05)；在子宮內(nèi)膜息肉組中，復(fù)雜型增生息肉組的VEGF陽性標(biāo)本百分率高于簡單增生型息肉組，差異具有統(tǒng)計學(xué)意義（x2=18.685，，P0.01），兩組在染色分值上沒有明顯差異（x2=2.685，P0.05）。 （2）MMP-2、MMP-9在復(fù)雜型增生息肉組和簡單增生型息肉組的表達(dá)明顯高于對照1組和對照2組，差異有統(tǒng)計學(xué)意義(P0.05)；MMP-9在對照1組和對照2組中的表達(dá)有明顯差異(P0.05)，而復(fù)雜增生型子宮內(nèi)膜息肉組的MMP-2、MMP-9陽性標(biāo)本百分率為91.2%（27/30）、88.2%（26/30），明顯高于簡單增生型息肉組陽性標(biāo)本百分率45.2%(14/30)、40.7%（12/30），而且其染色分值差異也具有統(tǒng)計學(xué)意義（x2=18.685，P0.01；x2=21.243，P0.05）。 （3）在子宮內(nèi)膜息肉組織中，VEGF、MMP-2、MMP-9三者表達(dá)呈正相關(guān)(F0.05)。 結(jié)論： （1） VEGF、MMP-2、MMP-9三者在子宮內(nèi)膜息肉組織高表達(dá)。 （2）復(fù)雜增生型子宮內(nèi)膜息肉VEGF、MMP-2、MMP-9蛋白表達(dá)高于簡單增生型子宮內(nèi)膜息肉。 （3）VEGF、MMP-2、MMP-9三者在子宮內(nèi)膜息肉蛋白表達(dá)呈正相關(guān)。
[Abstract]:Objective: to detect the expression of vascular endothelial growth factor (VEGF), matrix metalloproteinase (2(matrix) metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) in endometrial polyps (complex hyperplasia and simple hyperplasia, peripolyp endometrial tissue and parenchyma). Protein expression in normal endometrial tissue; To investigate the role and correlation of VEGF MMP-2 and MMP-9 in the pathogenesis of endometrial polyps. Methods: specimens were collected from 60 cases of endometrial polyps from December 2009 to December 2012 in the affiliated Hospital of Xiangnan University. 30 cases of complex proliferative endometrial polyps were confirmed as simple proliferative endometrial polyps. Then 30 samples of endometrial tissue adjacent to endometrial polyps were selected as control group and 30 cases of normal endometrial tissue as control group. Immunohistochemical staining: the expressions of VEGF MMP-2 and MMP-9 were observed by immunohistochemical staining in 30 cases of endometrial tissues adjacent to endometrial polyps and 30 cases of normal endometrium. Results: qualitative judgment was made according to the staining cells, the semi-quantitative method of the percentage of positive cells was used to determine the staining results, and in each case, the positive degree was determined by integral method: 0 ~ 2 points were negative. In 3, 5 were weakly positive (P < 0. 05), 8 were positive (P < 0. 05), 12 were strongly positive (P < 0. 05). All results were assessed independently by two pathologists. Statistical analysis: SPSS 19.0 statistical software package was used for statistical analysis. According to the nature of the data, different statistical methods are used: the comparison of two sample rates is made by using x2 test or fisher exact probability method, and the comparison of grade data is based on Mann-Whitney rank sum test. Correlation analysis using spearman correlation analysis, measurement data using t test and variance analysis. P0.05 as the statistical significance standard. Results: compared with control group 1 and control group 2, the expression of VEGF protein in endometrial polyp group was significantly higher than that in control group (P 0.05), including complex hyperplasia polyp group and simple proliferative polyp group. The percentage of VEGF positive specimens in complex polyps was higher than that in simple proliferative polyps. There was no significant difference in the staining scores between the two groups. The expression of MMP-2MMP-9 in the complex hyperplasia polyp group and simple proliferative polyp group was significantly higher than that in the control group and the control group (P 0.05), and the difference was statistically significant (P 0.05). The expression of MMP-9 in control group 1 was significantly higher than that in control group 2 (P 0.05), while the positive rate of MMP-2 + MMP-9 in complex proliferative endometrial polyps was 91. 2%, 27 / 30 and 88. 2 / 30 respectively, which was significantly higher than that in simple proliferative polyp group (45.214 / 3040.7) and the positive rate of MMP-9 was 12 / 30%, and the positive rate of MMP-9 was significantly higher than that of simple proliferative polyp group (45.214 / 3040.7%), and the percentage of MMP-9 positive specimens was 91.2%. The difference of score was also statistically significant (P 0.01). The expression of VEGF, MMP-2, MMP-9 in endometrial polyps was positively correlated with that of F0. 05. Conclusion: (1) the expression of MMP-2 MMP-9 in endometrial polyps is higher than that in simple proliferative endometrial polyps, and the expression of MMP-9 is higher in endometrial polyps than that in simple proliferative endometrial polyps. The expression of MMP-9 and MMP-9 in endometrial polyps is higher than that in simple proliferative endometrial polyps, and the expression of MMP-9 in endometrial polyps is higher than that in simple proliferative endometrial polyps. The expression of endometrial polyp protein was positively correlated.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R737.33

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