本文選題：VEGF + MMP-2��； 參考：《南華大學》2014年碩士論文

【摘要】：目的： 檢測血管內(nèi)皮生長因子(vaseularendothelialgrowthfaetor,VEGF)、基質(zhì)金屬蛋白酶2(matrix metalloproteinase2, MMP-2)和基質(zhì)金屬蛋白酶9(matrixmetalloproteinase9, MMP-9)在子宮內(nèi)膜息肉組織（復雜型增生及簡單型增生）、息肉旁內(nèi)膜組織及子宮正常內(nèi)膜組織中的蛋白表達；探討VEGF、MMP-2及MMP-9在子宮內(nèi)膜息肉發(fā)病機制中的作用及其相關(guān)性。 方法： 標本收集：收集湘南學院附屬醫(yī)院2009年12月到2012年12月60例確診為子宮內(nèi)膜息肉組織樣本為觀察組，其中經(jīng)確診有30例為復雜性增生型子宮內(nèi)膜息肉組織樣本，30例為簡單增生型子宮內(nèi)膜息肉組織樣本。然后再選擇同期子宮內(nèi)膜息肉旁的內(nèi)膜組織樣本30例為對照1組，選擇正常子宮內(nèi)膜組織樣本30例為對照2組。 免疫組化：取60例子宮內(nèi)膜息肉、30例子宮內(nèi)膜息肉旁的內(nèi)膜組織及30例正常子宮內(nèi)膜的組織石蠟標本，免疫組織化學染色，顯微鏡下觀察VEGF、MMP-2及MMP-9蛋白表達。 結(jié)果判斷：根據(jù)染色細胞進行定性判斷；采用陽性細胞百分率的半定量法來判定染色結(jié)果，在每例的染色結(jié)果中，陽性程度的判定采用積分方法：0~2分為陰性(-)；3~5分為弱陽性(+)；6~8分為陽性(++)；9~12分為強陽性(+++)。所有結(jié)果均由兩位病理醫(yī)師獨立評定。 統(tǒng)計分析：采用SPSS19.0統(tǒng)計軟件包進行統(tǒng)計學分析。根據(jù)資料性質(zhì)采用不同統(tǒng)計方法：兩樣本率的比較采用x2檢驗或fisher確切概率法；等級資料的比較采Mann-Whitney秩和檢驗。相關(guān)性分析采用spearman相關(guān)分析；計量資料采用t檢驗及方差分析。P0.05為統(tǒng)計學顯著性意義標準。 結(jié)果： （1）與對照1組和對照2組相比，子宮內(nèi)膜息肉組包括復雜型增生息肉組和簡單增生型息肉組VEGF蛋白表達增高，差異有統(tǒng)計學意義(P0.05)；在子宮內(nèi)膜息肉組中，復雜型增生息肉組的VEGF陽性標本百分率高于簡單增生型息肉組，差異具有統(tǒng)計學意義（x2=18.685，，P0.01），兩組在染色分值上沒有明顯差異（x2=2.685，P0.05）。 （2）MMP-2、MMP-9在復雜型增生息肉組和簡單增生型息肉組的表達明顯高于對照1組和對照2組，差異有統(tǒng)計學意義(P0.05)；MMP-9在對照1組和對照2組中的表達有明顯差異(P0.05)，而復雜增生型子宮內(nèi)膜息肉組的MMP-2、MMP-9陽性標本百分率為91.2%（27/30）、88.2%（26/30），明顯高于簡單增生型息肉組陽性標本百分率45.2%(14/30)、40.7%（12/30），而且其染色分值差異也具有統(tǒng)計學意義（x2=18.685，P0.01；x2=21.243，P0.05）。 （3）在子宮內(nèi)膜息肉組織中，VEGF、MMP-2、MMP-9三者表達呈正相關(guān)(F0.05)。 結(jié)論： （1） VEGF、MMP-2、MMP-9三者在子宮內(nèi)膜息肉組織高表達。 （2）復雜增生型子宮內(nèi)膜息肉VEGF、MMP-2、MMP-9蛋白表達高于簡單增生型子宮內(nèi)膜息肉。 （3）VEGF、MMP-2、MMP-9三者在子宮內(nèi)膜息肉蛋白表達呈正相關(guān)。
[Abstract]:Objective: to detect the expression of vascular endothelial growth factor (VEGF), matrix metalloproteinase (2(matrix) metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) in endometrial polyps (complex hyperplasia and simple hyperplasia, peripolyp endometrial tissue and parenchyma). Protein expression in normal endometrial tissue; To investigate the role and correlation of VEGF MMP-2 and MMP-9 in the pathogenesis of endometrial polyps. Methods: specimens were collected from 60 cases of endometrial polyps from December 2009 to December 2012 in the affiliated Hospital of Xiangnan University. 30 cases of complex proliferative endometrial polyps were confirmed as simple proliferative endometrial polyps. Then 30 samples of endometrial tissue adjacent to endometrial polyps were selected as control group and 30 cases of normal endometrial tissue as control group. Immunohistochemical staining: the expressions of VEGF MMP-2 and MMP-9 were observed by immunohistochemical staining in 30 cases of endometrial tissues adjacent to endometrial polyps and 30 cases of normal endometrium. Results: qualitative judgment was made according to the staining cells, the semi-quantitative method of the percentage of positive cells was used to determine the staining results, and in each case, the positive degree was determined by integral method: 0 ~ 2 points were negative. In 3, 5 were weakly positive (P < 0. 05), 8 were positive (P < 0. 05), 12 were strongly positive (P < 0. 05). All results were assessed independently by two pathologists. Statistical analysis: SPSS 19.0 statistical software package was used for statistical analysis. According to the nature of the data, different statistical methods are used: the comparison of two sample rates is made by using x2 test or fisher exact probability method, and the comparison of grade data is based on Mann-Whitney rank sum test. Correlation analysis using spearman correlation analysis, measurement data using t test and variance analysis. P0.05 as the statistical significance standard. Results: compared with control group 1 and control group 2, the expression of VEGF protein in endometrial polyp group was significantly higher than that in control group (P 0.05), including complex hyperplasia polyp group and simple proliferative polyp group. The percentage of VEGF positive specimens in complex polyps was higher than that in simple proliferative polyps. There was no significant difference in the staining scores between the two groups. The expression of MMP-2MMP-9 in the complex hyperplasia polyp group and simple proliferative polyp group was significantly higher than that in the control group and the control group (P 0.05), and the difference was statistically significant (P 0.05). The expression of MMP-9 in control group 1 was significantly higher than that in control group 2 (P 0.05), while the positive rate of MMP-2 + MMP-9 in complex proliferative endometrial polyps was 91. 2%, 27 / 30 and 88. 2 / 30 respectively, which was significantly higher than that in simple proliferative polyp group (45.214 / 3040.7) and the positive rate of MMP-9 was 12 / 30%, and the positive rate of MMP-9 was significantly higher than that of simple proliferative polyp group (45.214 / 3040.7%), and the percentage of MMP-9 positive specimens was 91.2%. The difference of score was also statistically significant (P 0.01). The expression of VEGF, MMP-2, MMP-9 in endometrial polyps was positively correlated with that of F0. 05. Conclusion: (1) the expression of MMP-2 MMP-9 in endometrial polyps is higher than that in simple proliferative endometrial polyps, and the expression of MMP-9 is higher in endometrial polyps than that in simple proliferative endometrial polyps. The expression of MMP-9 and MMP-9 in endometrial polyps is higher than that in simple proliferative endometrial polyps, and the expression of MMP-9 in endometrial polyps is higher than that in simple proliferative endometrial polyps. The expression of endometrial polyp protein was positively correlated.
【學位授予單位】：南華大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.33

【參考文獻】

相關(guān)期刊論文 前10條

1 陳美如;鄭玉容;李麗妮;;子宮內(nèi)膜息肉的手術(shù)治療及術(shù)后復發(fā)的預防[J];當代醫(yī)學;2011年27期

2 湯惠茹;王麗平;陳曉琳;王玉潔;吳瑞芳;;子宮內(nèi)膜息肉的手術(shù)治療及術(shù)后復發(fā)的預防[J];中國婦產(chǎn)科臨床雜志;2010年03期

3 孫藜;王樹鶴;;子宮內(nèi)膜息肉發(fā)病機制及易患因素研究進展[J];中國婦產(chǎn)科臨床雜志;2011年05期

4 張詩武,高欣;血管生成擬態(tài)和血管生成及其意義[J];國外醫(yī)學(腫瘤學分冊);2003年03期

5 紀統(tǒng)慧;孫麗洲;戴輝華;王秀麗;;環(huán)氧化酶-2及細胞色素芳香化酶基因mRNA在育齡婦女子宮內(nèi)膜息肉中的表達[J];現(xiàn)代中西醫(yī)結(jié)合雜志;2009年26期

6 周林;徐岬;康建芳;;基質(zhì)金屬蛋白酶-2、基質(zhì)金屬蛋白酶-9和血管內(nèi)皮生長因子在視網(wǎng)膜母細胞瘤中的表達及意義[J];眼科學報;2010年01期

7 宗瑞平;;Bcl-2基因及其與子宮內(nèi)膜癌的關(guān)系[J];醫(yī)學理論與實踐;2006年04期

8 武力;王新允;;子宮內(nèi)膜息肉的相關(guān)因素探討[J];醫(yī)學綜述;2007年11期

9 孟麗;趙肖麗;李芳;任秀麗;趙靜;;宮腔鏡治療子宮內(nèi)膜息肉及術(shù)后復發(fā)的預防[J];中國內(nèi)鏡雜志;2012年01期

10 趙蔚;常才;;子宮內(nèi)膜息肉及其診斷方法[J];中國實用婦科與產(chǎn)科雜志;2008年10期

相關(guān)博士學位論文 前1條

1 何淑明;宮頸、子宮內(nèi)膜病變患者宮頸、陰道分泌物CA125表達機理及臨床意義[D];南方醫(yī)科大學;2010年

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