本文選題：Twist + KiSS-1　； 參考：《瀘州醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的：通過檢測(cè)Twist和KiSS-1在正常宮頸、宮頸上皮內(nèi)瘤變（CIN）及宮頸鱗癌（squamous cervical cancer,SCC）組織中的表達(dá)情況，探討二者在宮頸鱗癌發(fā)生發(fā)展中的作用及其意義。方法：采用免疫組織化學(xué)Envison法檢測(cè)12例正常宮頸（normal cervical epithelium，NCE）,20例宮頸上皮內(nèi)瘤變（cervical intraepithelial neoplasia，CIN)及47例宮頸鱗癌組織中Twist和KiSS-1的表達(dá)情況，分析二者的表達(dá)與宮頸癌患者各臨床病理因素的相關(guān)性。采用χ2檢驗(yàn)或Fisher’s精確概率法比較計(jì)數(shù)資料間的差異，采用Spearman法進(jìn)行相關(guān)性檢驗(yàn)，生存曲線采用Kaplan-Meier，生存率比較采用Log-rank檢驗(yàn)，COX比例風(fēng)險(xiǎn)模型進(jìn)行多因素分析。結(jié)果：1、Twist在正常宮頸、CIN中主要表達(dá)于細(xì)胞核，而在SCC中主要表達(dá)于細(xì)胞質(zhì)中。Twist在正常宮頸、CIN、SCC中的表達(dá)呈逐漸上升趨勢(shì)，其陽性表達(dá)率分別為：16.67%（2/12）、30%（6/20）、85.11%（40/47），三組間比較，差異有統(tǒng)計(jì)學(xué)意義（χ2＝29.407，P=0.0000.05）；Twist在SCC中的陽性表達(dá)率與正常宮頸組中的陽性表達(dá)率比較，差異有統(tǒng)計(jì)學(xué)意義（χ2＝21.830，P=0.0000.05）；Twist在SCC組織中的陽性表達(dá)率與CIN中的陽性表達(dá)率比較，差異有統(tǒng)計(jì)學(xué)意義（χ2＝19.798，P=0.0000.05）。Twist在宮頸癌患者不同臨床特征組中，如患者年齡、FIGO分期、組織分化程度、淋巴結(jié)轉(zhuǎn)移、間質(zhì)浸潤深度等的表達(dá)差異無統(tǒng)計(jì)學(xué)意義(P0.05)，但有淋巴結(jié)轉(zhuǎn)移者比無轉(zhuǎn)移者有較高的Twist表達(dá)，其中無淋巴結(jié)轉(zhuǎn)移者陽性表達(dá)為81.08%，有淋巴結(jié)轉(zhuǎn)移者為100%，經(jīng)統(tǒng)計(jì)學(xué)分析，χ2＝2.223，P=0.3180.05，其差異無統(tǒng)計(jì)學(xué)意義。2、KiSS-1在宮頸上皮、SCC中主要表達(dá)于細(xì)胞膜和細(xì)胞質(zhì)，胞核中亦有少量表達(dá)。KiSS-1在正常宮頸組、CIN組、SCC組中的表達(dá)呈逐漸下降的趨勢(shì)，其陽性表達(dá)率分別為：91.67%（11/12）、70.00%（14/20）、27.7%（13/47），，三組間比較，差異有統(tǒng)計(jì)學(xué)意義（χ2＝20.832，P=0.0000.05）；KiSS-1在SCC組中的陽性表達(dá)率與正常宮頸組中的陽性表達(dá)率比較，差異有統(tǒng)計(jì)學(xué)意義（χ2＝38.417，P=0.0000.05）； KiSS-1在SCC組中的陽性表達(dá)率與CIN組中的陽性表達(dá)率比較，差異有統(tǒng)計(jì)學(xué)意義（χ2＝29.987，P=0.0000.05）。KiSS-1在宮頸癌不同臨床特征組中，如患者年齡、FIGO分期、組織分化程度、淋巴結(jié)轉(zhuǎn)移、間質(zhì)浸潤深度等的表達(dá)差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。3、Twist和KiSS-1在宮頸鱗癌組織中的表達(dá)無相關(guān)性（rs=0.027，P=0.8590.05）。4、單因素及多因素分析結(jié)果顯示Twist及KiSS-1的表達(dá)與宮頸鱗癌患者的預(yù)后無關(guān)（P值均0.05）。結(jié)論：1. Twist在正常宮頸組織、CIN及宮頸鱗癌組織中的表達(dá)呈依次升高趨勢(shì)，說明Twist可能與宮頸鱗癌的發(fā)生發(fā)展有關(guān)。Twist在宮頸鱗癌中的表達(dá)與年齡、FIGO分期、組織學(xué)分級(jí)、間質(zhì)浸潤深度及有無淋巴結(jié)轉(zhuǎn)移等臨床病理因素?zé)o關(guān)。2.KiSS-1在正常宮頸組織、CIN及宮頸鱗癌組織中的表達(dá)呈依次降低趨勢(shì)，說明KiSS-1的低表達(dá)與宮頸鱗癌的發(fā)生發(fā)展可能相關(guān)。KiSS-1在宮頸鱗癌中的表達(dá)與年齡、FIGO分期、組織學(xué)分級(jí)、間質(zhì)浸潤深度及有無淋巴結(jié)轉(zhuǎn)移等臨床病理因素?zé)o關(guān)。3. Twist的表達(dá)及KiSS-1的表達(dá)與宮頸鱗癌患者的預(yù)后無關(guān)。4. Twist及KiSS-1在宮頸鱗癌中的表達(dá)無相關(guān)性。
[Abstract]:Objective: To investigate the role and significance of the expression of Twist and KiSS-1 in the normal cervix, cervical intraepithelial neoplasia (CIN) and squamous cervical cancer (SCC), and to explore the role and significance of the two cases in the development of cervical squamous cell carcinoma. Methods: 12 cases of normal cervix (normal cervical epith) were detected by immunohistochemical Envison method. Elium, NCE), the expression of Twist and KiSS-1 in 20 cases of cervical intraepithelial neoplasia (cervical intraepithelial neoplasia, CIN) and 47 cases of cervical squamous cell carcinoma. The correlation between the expression of the two and the clinicopathological factors of the patients with cervical cancer was analyzed. The difference between the count data was compared by the chi 2 test or the Fisher 's precision method, and Spearman was used. The correlation test was carried out, the survival curve was Kaplan-Meier, the survival rate was compared with the Log-rank test and the COX proportional risk model was used for multiple factors analysis. Results: 1, Twist was in the normal cervix and CIN was mainly expressed in the nucleus, while the expression of.Twist in the cytoplasm was gradually rising in the normal cervix, CIN, and SCC in the cytoplasm. The positive expression rates were 16.67% (2/12), 30% (6/20) and 85.11% (40/47). The difference between the three groups was statistically significant (x 2 = 29.407, P=0.0000.05), and the positive rate of Twist in SCC was compared with the positive rate in the normal cervix group (x 2 = 21.830, P=0.0000.05), and Twist was positive in SCC tissues. Compared with the positive expression rate in CIN, the difference was statistically significant (x 2 = 19.798, P=0.0000.05).Twist in different clinical characteristics of cervical cancer patients, such as patients' age, FIGO staging, degree of differentiation, lymph node metastasis, interstitial infiltration depth, and so on (P0.05), but there was no transfer of lymph node metastases. The positive expression of the Twist was 81.08% and the lymph node metastasis was 100%, and the difference was 2.223, P=0.3180.05. The difference was not statistically significant.2, KiSS-1 was in the cervical epithelium, SCC was mainly expressed in the cell membrane and the cell cytoplasm, and a small amount of.KiSS-1 was expressed in the normal cervix group. In group CIN, the expression in group SCC showed a trend of gradual decline. The positive expression rates were 91.67% (11/12), 70% (14/20) and 27.7% (13/47). The difference was statistically significant (x 2 = 20.832, P=0.0000.05), and the positive rate of KiSS-1 in the SCC group was compared with the positive rate in the normal cervical group, the difference was statistically significant. 2 = 38.417, P=0.0000.05); the positive expression rate of KiSS-1 in group SCC compared with the positive expression rate in group CIN was statistically significant (x 2 = 29.987, P=0.0000.05) in different clinical features of cervical cancer, such as patients' age, FIGO stage, tissue differentiation, lymph node metastasis, interstitial infiltration depth and so on Statistical significance (P0.05).3, Twist and KiSS-1 expression in cervical squamous cell carcinoma (rs=0.027, P=0.8590.05).4. Single factor and multivariate analysis showed that the expression of Twist and KiSS-1 was not related to the prognosis of cervical squamous cell carcinoma (P value was 0.05). Conclusion: 1. Twist in normal cervical tissues, CIN, and cervical squamous cell carcinoma tissue The trend in turn indicates that Twist may be associated with the development of cervical squamous cell carcinoma and the expression and age of.Twist in cervical squamous cell carcinoma, FIGO staging, histological grading, interstitial infiltration depth and lymph node metastasis are not related to.2.KiSS-1 in normal cervical tissue, and the expression of CIN and cervical squamous cell carcinoma is decreasing in turn. The expression of KiSS-1 and the development of cervical squamous cell carcinoma may be related to the expression and age of.KiSS-1 in cervical squamous cell carcinoma, FIGO staging, histological grading, interstitial infiltration depth and lymph node metastasis are not related to the expression of.3. Twist and the expression of KiSS-1 and the prognosis of cervical squamous carcinoma is not related to.4. Twist and KiSS-1. There is no correlation in the expression in squamous cell carcinoma of the cervix.
【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.33

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