發(fā)布時(shí)間：2018-06-06 20:17

  本文選題：卵巢癌 + O-GlcNAc　； 參考：《現(xiàn)代婦產(chǎn)科進(jìn)展》2017年07期

【摘要】：目的:探討O-GLcNAc糖基化修飾對(duì)卵巢癌細(xì)胞內(nèi)β-catenin水平以及細(xì)胞增殖的影響。方法:利用慢病毒轉(zhuǎn)染技術(shù)構(gòu)建O-GLcNAc低表達(dá)SKOV3細(xì)胞模型。利用O-GLcNAc水解酶(OGA)抑制劑PUGNAc構(gòu)建高表達(dá)A2780細(xì)胞模型。流式細(xì)胞技術(shù)檢測(cè)構(gòu)建O-GLcNAc糖基化對(duì)細(xì)胞周期的影響。MTT法檢測(cè)O-GLcNAc糖基化對(duì)細(xì)胞增殖的影響。Westernblot法檢測(cè)不同細(xì)胞模型內(nèi)β-catenin表達(dá)情況。CO-IP法檢測(cè)不同細(xì)胞模型內(nèi)β-catenin的糖基化修飾水平。結(jié)果:下調(diào)細(xì)胞內(nèi)O-GLcNAc糖基化水平能抑制細(xì)胞的增殖能力,上調(diào)細(xì)胞內(nèi)O-GLcNAc糖基化水平能增強(qiáng)細(xì)胞的增殖能力。下調(diào)細(xì)胞內(nèi)O-GLcNAc糖基化水平后,β-catenin表達(dá)以及糖基化修飾水平降低;上調(diào)細(xì)胞內(nèi)O-GLcNAc糖基化水平后,β-catenin表達(dá)以及糖基化修飾水平增高。結(jié)論:卵巢癌細(xì)胞內(nèi)OGLcNAc糖基化水平變化能通過(guò)β-catenin對(duì)細(xì)胞增殖產(chǎn)生影響。
[Abstract]:Aim: to investigate the effect of O-GLcNAc glycosylation on 尾 -catenin level and cell proliferation in ovarian cancer cells. Methods: O-GLcNAc low expression SKOV3 cell model was constructed by lentivirus transfection technique. The high expression A2780 cell model was constructed by using O-GLcNAc hydrolase (OGA) inhibitor PUGNAc. Flow cytometry was used to detect the effect of glycosylation of O-GLcNAc on cell cycle. MTT assay was used to detect the effect of O-GLcNAc glycosylation on cell proliferation. Western blot was used to detect the expression of 尾 -catenin in different cell models. CO-IP method was used to detect the glycosylation modification level of 尾 -catenin in different cell models. Results: down-regulating the level of O-GLcNAc glycosylation could inhibit the proliferation of cells, and up-regulate the level of O-GLcNAc glycosylation could enhance the proliferation of cells. After down-regulating the glycosylation level of O-GLcNAc, the expression of 尾 -catenin and the level of glycation modification decreased, but the expression of 尾 -catenin and the level of glycosylation increased after up-regulating the level of glycosylation of O-GLcNAc. Conclusion: the change of OGLcNAc glycosylation level in ovarian cancer cells can affect cell proliferation through 尾-catenin.
【作者單位】： 第四軍醫(yī)大學(xué)西京醫(yī)院婦產(chǎn)科;寧夏軍區(qū)醫(yī)院婦產(chǎn)科;寧夏醫(yī)科大學(xué);
【分類號(hào)】：R737.31

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