本文選題：活性氧 + 精液質(zhì)量�。� 參考：《廣西醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：研究通過測定精液活性氧濃度與精液常規(guī)參數(shù)及與體外受精結(jié)局的關(guān)系，分析活性氧含量與精液質(zhì)量及體外受精是否存在相關(guān)性，評估精液活性氧在男性不育診斷及治療中的應(yīng)用價(jià)值。 方法：資料來自2012年10至2013年1月因輸卵管因素不孕在本中心初次接受IVF-ET診療的72對夫婦，運(yùn)用Diff-Quik精子形態(tài)學(xué)分析法進(jìn)行嚴(yán)格精子形態(tài)學(xué)分析，用苯胺藍(lán)染色法進(jìn)行精子核成熟度檢測，化學(xué)發(fā)光法檢測精液活性氧含量，于體外受精第一天觀察受精情況，第三天觀察受精卵卵裂及優(yōu)質(zhì)胚胎情況。Pearson相關(guān)分析法分析精液活性氧含量與精液質(zhì)量及IVF結(jié)局的關(guān)系。 結(jié)果:72例患者精液活性氧含量(72.19±3.56)RLU/s。Pearson相關(guān)分析顯示精液活性氧含量與精子活率、正常形態(tài)率、受精率、正常受精率均呈顯著負(fù)相關(guān)(r=-0.799、-0.827、-0.768、-0.792，P=0.012、0.032、0.000、0.003，均0.05)，與精子密度、不成熟精子率呈正相關(guān)(r=0.317、0.794，P=0.008、0.001，均0.05)，與男方年齡、精液量及IVF卵裂率、優(yōu)胚率無相關(guān)性(P均0.05)。 結(jié)論：精液活性氧含量與精液質(zhì)量及體外受精結(jié)局有相關(guān)性。適量活性氧在精子的發(fā)生、成熟和獲能具有一定的作用，然而，過量活性氧可致精子活力下降，，受精率降低；但對IVF卵裂率和優(yōu)質(zhì)胚胎率無顯著影響。 目的:研究體外離心對正常精子與少弱精子的氧化應(yīng)激損傷；探討抗氧化劑谷胱甘肽、左卡尼汀在精子體外離心過程中抗氧化應(yīng)激損傷的作用效果。 方法:按WHO標(biāo)準(zhǔn)選取35份正常精子樣本和38份少弱精子樣本，每份樣本各取1800ul，分為空白對照組、離心對照組、谷胱甘肽離心組和左卡尼汀離心組，各450ul。對照組：僅加入EBSS平衡液不離心；離心對照組：加入EBSS平衡液并離心；谷胱甘肽離心組：加入含有一定濃度谷胱甘肽的EBSS平衡液并離心；左卡尼汀離心組：加入含有一定濃度左卡尼汀的EBSS平衡液并離心，檢測各組活性氧（ROS）、丙二醛（MDA）及精子DNA斷裂指數(shù)（DFI）。統(tǒng)計(jì)檢驗(yàn)采用隨機(jī)區(qū)組設(shè)計(jì)方差分析并做組間兩兩比較。 結(jié)果:1.四組組間比較均有統(tǒng)計(jì)學(xué)意義。正常精子組：FROS=280.10、P=0.000；FMDA=15.98，P=0.001；FDFI=74.84，P=0.002，P均0.05；少弱精子組：FROS=9.45、P=0.000；FMDA=15.79，P=0.000；FDFI=13.56，P=0.000，P均0.05。2.兩兩比較示正常精子組和少弱精子組的離心對照組ROS、MDA及DFI水平較空白對照組均明顯升高(P均0.05)；谷胱甘肽離心組與左卡尼汀離心組的ROS、MDA及DFI水平均較離心對照組間明顯降低，差異有統(tǒng)計(jì)學(xué)意義(P均0.05)；而谷胱甘肽離心組及左卡尼汀離心組間的比較均無統(tǒng)計(jì)學(xué)意義(P均0.05))。 結(jié)論:正常精子和少弱精子在離心處理過程中均會(huì)產(chǎn)生過量活性氧導(dǎo)致過氧化損傷;在精液離心前添加一定濃度的左卡尼汀或谷胱甘肽可減少離心過程中產(chǎn)生的過量活性氧對精子的氧化應(yīng)激性損傷，從而提高精子質(zhì)量。
[Abstract]:Objective: to study the relationship between the concentration of reactive oxygen species (Ros) in semen and semen routine parameters and the outcome of in vitro fertilization (IVF), and to analyze the correlation between Ros content and semen quality and in vitro fertilization (IVF). To evaluate the value of semen reactive oxygen species (Ros) in the diagnosis and treatment of male infertility. Methods: from October 2012 to January 2013, 72 couples who were first treated with IVF-ET because of fallopian tube infertility were analyzed by Diff-Quik sperm morphology analysis. The sperm nuclear maturity was detected by aniline blue staining, the active oxygen species in semen was detected by chemiluminescence, and the fertilization was observed on the first day of in vitro fertilization. On the third day, the cleavage of fertilized eggs and high quality embryos were observed. Pearson correlation analysis was used to analyze the relationship between the content of Ros and the quality of semen and the outcome of IVF. Results the results of 72.19 鹵3.56)RLU/s.Pearson correlation analysis showed that there was a significant negative correlation between semen Ros content and sperm motility, normal morphology, fertilization rate, normal fertilization rate, and normal fertilization rate. There was a significant negative correlation between Ros content and sperm density in 72 patients with Ros 0.012, 0. 032, 0. 003, 0. 05, 0. 05, and 0. 779 9, 0. 827, 0. 768 and 0. 792%, 0. 032, 0. 000, 0. 003, 0. 05, 0. 05, 0. 05, 0. 05, 0. 05 respectively. There was a positive correlation between immature sperm rate and age, semen volume and cleavage rate of IVF, and there was no correlation between the rate of superior embryo and the age of male, sperm volume and cleavage rate of IVF, and there was no correlation between the rate of superior embryo and the age of male side, the quantity of spermatozoa and the rate of cleavage of IVF. Conclusion: the content of Ros in semen is correlated with the quality of semen and the outcome of in vitro fertilization. Proper amount of Ros had certain effect on spermatogenesis, maturation and capacitation. However, excessive Ros could decrease sperm motility and fertilization rate, but had no significant effect on IVF cleavage rate and high quality embryo rate. Aim: to investigate the effects of antioxidant glutathione and levacarnitine on oxidative stress injury of normal sperm and oligozoospermia during centrifugation in vitro. Methods: according to WHO standard, 35 normal sperm samples and 38 oligozoospermia samples were divided into three groups: blank control group, centrifugal control group, glutathione centrifugation group and levacarnitine centrifugation group, each with 450 ululins. The control group only added EBSS equilibrium solution without centrifugation, the centrifugal control group added EBSS equilibrium solution and centrifugation, the glutathione centrifugation group added EBSS equilibrium solution containing a certain concentration of glutathione and centrifugation. Levacarnitine centrifugation group: the EBSS equilibrium solution containing a certain concentration of L-carnitine was added and centrifuged. The reactive oxygen species (Ros), malondialdehyde (MDA) and sperm DNA breakage index (DNA) were detected in each group. Random block design ANOVA was used to test the statistical results. The result is 1: 1. There were significant differences among the four groups. Normal spermatozoa group: normal spermatozoa group (280.10) P0. 000% FMDAA 15.98% P0. 001 FDFI (74.84) (P = 0.002) P, P = 0. 05; in oligozoospermia group, it is 9. 45% P0. 000 FROS0. 000% FROSU 15. 79P0. 000FDFI 13.56P0. 000 (P = 0. 05.2p = 0. 05.2P = 0. 05.2P = 0. 05.2P = 0. 05.2P = 0. 05.2P = 0.05.2respectively). The results showed that the levels of rosid MDA and DFI in normal sperm group and oligozoospermia group were significantly higher than those in blank control group, while the levels of rosid MDA and DFI in glutathione centrifugation group and leucarnitine centrifugation group were significantly lower than those in control group. The difference was statistically significant (P < 0.05), but there was no significant difference between glutathione centrifugation group and L-carnitine centrifugation group (P < 0.05). Conclusion: both normal spermatozoa and oligozoospermia can produce excessive reactive oxygen species (Ros) and lead to peroxidation damage during centrifugation. Adding a certain concentration of leucarnitine or glutathione to semen before centrifugation can reduce the oxidative stress damage of spermatozoa produced by excessive reactive oxygen species during centrifugation and improve the quality of spermatozoa.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R714.8

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