本文選題：陰道壁 + 脫細胞基質�。� 參考：《上海交通大學學報(醫(yī)學版)》2017年10期

【摘要】：目的·探討人陰道壁脫細胞基質的納米/微米級結構特點,為生物材料制備仿生人工陰道提供參考數(shù)據(jù)。方法·對35例陰道壁脫垂的絕經(jīng)后患者盆底重建手術中修剪下的陰道壁組織進行脫細胞處理,制備陰道壁脫細胞基質,并對脫細胞基質進行蘇木精-伊紅(H-E)染色、Masson染色、電鏡掃描和Micro-CT掃描,采集并分析陰道壁脫細胞基質的大體形態(tài)及定量數(shù)據(jù)。結果·35例陰道壁標本脫細胞完全,基本無細胞殘留。陰道壁脫細胞基質由互相交錯的膠原纖維構成,為納米級膠原纖維構成的雙層纖維網(wǎng)狀結構。膠原纖維呈束狀排列,纖維束之間分布孔隙。陰道壁脫細胞基質上皮側結構致密,為致密層;上皮下組織結構疏松,為疏松層。致密層和疏松層的纖維絲直徑無顯著差異[(82.4±9.3)nm vs(87.5±10.2)nm,P=0.432],纖維束寬度無顯著差異[(67.6±9.3)μm vs(65.3±5.2)μm,P=0.634],疏松層的纖維束分離度較致密層大[(180.1±24.5)μm vs(118.2±23.0)μm,P=0.003],疏松層的孔隙率較致密層高[(77.1±4.2)%vs(66.6±2.8)%,P=0.002]。陰道前壁和陰道后壁的脫細胞基質的微納結構參數(shù)沒有顯著差異。結論·人陰道壁脫細胞基質致密層和疏松層的纖維絲直徑、纖維束寬度無顯著差異,但致密層纖維束的孔隙率較疏松層小。
[Abstract]:Objective to study the nanometer / micron structure of human vaginal wall acellular matrix and to provide reference data for the preparation of biomimetic artificial vagina. Methods 35 postmenopausal patients with vaginal wall prolapse were treated with acellular tissue of vaginal wall trimmed during pelvic floor reconstruction, and the acellular matrix of vaginal wall was prepared, and the acellular matrix was stained by Masson staining with hematoxylin-eosin H-E staining. The morphological and quantitative data of acellular matrix of vaginal wall were collected and analyzed by electron microscopy and Micro-CT scanning. Results 35 cases of vaginal wall specimens were completely acellular and almost no cell residue. The acellular matrix of vaginal wall is composed of interlaced collagen fibers, which is a double fiber reticular structure composed of nanometer collagen fibers. The collagenous fibers were arranged in bundles, and the pores were distributed between the fibrous bundles. The acellular matrix epithelium of the vaginal wall is compact and the upper subcutaneous tissue is loose, which is the loose layer. There was no significant difference in fiber diameter between dense layer and loose layer [82.4 鹵9.3)nm vs(87.5 鹵10.2nmP0. 432], and there was no significant difference in the width of fiber bundle [67.6 鹵9.3) 渭 m vs(65.3 鹵5.2 渭 m P0. 634]. The separation degree of fiber bundle in loose layer was larger than that in dense layer [180.1 鹵24. 5 渭 m vs(118.2 鹵23. 0 渭 m P0. 003], and the porosity of loose layer was higher than that of dense layer [77.1 鹵4.2)%vs(66.6 鹵2. 8]. There was no significant difference in microstructural parameters of acellular matrix between anterior wall of vagina and posterior wall of vagina. Conclusion there is no significant difference in the diameter and width of fiber bundle between acellular matrix dense layer and loose layer of human vaginal wall, but the porosity of dense layer fiber bundle is smaller than that of loose layer.
【作者單位】： 上海交通大學附屬第六人民醫(yī)院婦產(chǎn)科;山東省臨沂市婦女兒童醫(yī)院婦產(chǎn)科;
【基金】：國家自然科學基金(81550008)~~
【分類號】：R713
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本文編號：1916700