本文選題：卵巢腫瘤 + 肝X受體�。� 參考：《山東大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年01期

【摘要】：目的檢測(cè)肝X受體蛋白在卵巢癌組織及正常卵巢組織中的表達(dá),探討肝X受體激動(dòng)劑T0901317對(duì)卵巢癌細(xì)胞系SKOV3細(xì)胞活力及凋亡的影響。方法收集2015年8月至2016年8月行初次手術(shù)治療的51例卵巢癌患者的組織標(biāo)本,另選取32例因子宮肌瘤等良性病變切除的正常卵巢組織作對(duì)照。采用免疫組織化學(xué)方法檢測(cè)肝X受體蛋白在卵巢癌組織及正常卵巢組織中的表達(dá);CCK8檢測(cè)T0901317對(duì)卵巢癌細(xì)胞系SKOV3增殖的影響;流式細(xì)胞術(shù)檢測(cè)T0901317對(duì)卵巢癌細(xì)胞系SKOV3凋亡的影響。結(jié)果肝X受體蛋白在正常卵巢組織較卵巢癌組織表達(dá)量高,兩組比較差異有統(tǒng)計(jì)學(xué)意義(P0.001),T0901317抑制卵巢癌細(xì)胞系SKOV3增殖具有明顯的劑量依賴性和時(shí)間依賴性(P0.05),T0901317促進(jìn)SKOV3凋亡具有明顯的劑量依賴性(P0.05)。結(jié)論肝X受體表達(dá)下調(diào)可能與卵巢癌的發(fā)生發(fā)展有關(guān),激活肝X受體可能會(huì)成為卵巢癌潛在的治療靶點(diǎn)。
[Abstract]:Objective to detect the expression of liver X receptor protein in ovarian cancer and normal ovarian tissues, and to investigate the effects of T0901317, a hepatic X receptor agonist, on the viability and apoptosis of ovarian cancer cell line SKOV3. Methods from August 2015 to August 2016, the tissue specimens of 51 patients with ovarian cancer underwent initial surgical treatment were collected and 32 normal ovarian tissues were selected as control group, which were excised from benign lesions such as uterine leiomyoma. The expression of liver X receptor protein in ovarian cancer and normal ovarian tissues was detected by immunohistochemical method. The effect of T0901317 on the proliferation of ovarian cancer cell line SKOV3 and the effect of T0901317 on apoptosis of ovarian cancer cell line SKOV3 were detected by flow cytometry. Results the expression of X receptor protein in normal ovary was higher than that in ovarian cancer. There was a significant difference between the two groups in the inhibition of SKOV3 proliferation by P0.001T0901317 in a dose-dependent and time-dependent manner. P0.05T0901317 promoted SKOV3 apoptosis in a dose-dependent manner. Conclusion the down-regulation of liver X receptor expression may be related to the occurrence and development of ovarian cancer, and activation of liver X receptor may be a potential therapeutic target for ovarian cancer.
【作者單位】： 山東大學(xué)齊魯醫(yī)院婦產(chǎn)科;
【基金】：山東省重點(diǎn)研發(fā)計(jì)劃(2015GGE27352)
【分類號(hào)】：R737.31

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 方峻,魏文寧,劉仲萍,宋善俊;人組織因子基因表達(dá)載體的構(gòu)建及其在人卵巢癌細(xì)胞系中的表達(dá)[J];中國(guó)實(shí)驗(yàn)血液學(xué)雜志;2003年06期

2 吳尚輝,鄧朝暉,祝斌,顧煥華,羅學(xué)濱,祝和成;一株新的卵巢癌細(xì)胞系的建立及其生物學(xué)特性分析[J];實(shí)用預(yù)防醫(yī)學(xué);2005年01期

3 李曉艷;王澤華;;卵巢癌細(xì)胞系中環(huán)氧化酶2的表達(dá)研究[J];邯鄲醫(yī)學(xué)高等�？茖W(xué)校學(xué)報(bào);2006年02期

4 程繼文;蔡捷;鄺曉聰;陳心秋;唐步堅(jiān);黃清華;劉燕;李力;;腹水來(lái)源卵巢癌細(xì)胞系的建立及其生物學(xué)特性[J];癌癥;2009年03期

5 徐紅梅;夏美慧;梁作文;鐘加滕;趙雪儉;何津;;卵巢癌細(xì)胞系中差異表達(dá)蛋白的相關(guān)性研究[J];中國(guó)婦幼保健;2010年07期

6 盧建,張金山,牟瀚舟,許沈華;糖皮質(zhì)激素對(duì)人卵巢癌細(xì)胞系增殖的抑制作用[J];中華婦產(chǎn)科雜志;1996年03期

7 徐明娟,劉宇健,宋亮年,王昭梅,張瓊;視黃酸對(duì)人卵巢癌細(xì)胞系3AO增殖分化的影響[J];第二軍醫(yī)大學(xué)學(xué)報(bào);1997年05期

8 顧琳慧;鄭智國(guó);陳魯;許沈華;;高低轉(zhuǎn)移人乳頭狀漿液性卵巢癌細(xì)胞系蛋白表達(dá)差異及其意義[J];中國(guó)實(shí)用婦科與產(chǎn)科雜志;2009年11期

9 王晶;雷利娜;尹洪麗;;透明質(zhì)酸結(jié)合蛋白1在人卵巢癌細(xì)胞系中表達(dá)及其與紫杉醇敏感性的關(guān)系[J];中國(guó)實(shí)用婦科與產(chǎn)科雜志;2012年01期

10 王建六，錢和年，李文錦，，馮捷;人卵巢癌細(xì)胞系在不同品系免疫小鼠腎包膜下生長(zhǎng)規(guī)律的探討[J];現(xiàn)代婦產(chǎn)科進(jìn)展;1995年04期

相關(guān)會(huì)議論文 前10條

1 鄭智國(guó);高

本文編號(hào)：1913320