本文選題：宮頸鱗狀細胞癌 + 浸潤類型　； 參考：《寧夏醫(yī)科大學》2017年碩士論文

【摘要】：目的宮頸癌(cervical cancer,CC)是女性常見的癌癥之一,為全球女性最常見癌癥的第二位,女性惡性腫瘤死亡率排行第三位,在發(fā)展中國家,是癌癥死亡第二個最常見的原因。其中,宮頸鱗狀細胞癌(squamous cell carcinoma,SCC)約占宮頸癌的75-80%,宮頸癌的浸潤方式及程度很大程度上決定著其預后與治療。鑒于宮頸SCC比例高且危害極大,急需進一步深刻認識其生物學特征及發(fā)生機理,為新的治療方法提供科學依據(jù)。人類多種腫瘤組織的免疫組化研究表明,絕大多數(shù)腫瘤相關巨噬細胞M2計數(shù)高的腫瘤患者的臨床預后較差,因此,研究腫瘤微環(huán)境中腫瘤相關巨噬細胞,應當特別關注M2。本課題首次研究了腫瘤相關巨噬細胞M2與宮頸SCC不同浸潤方式(invasion patterns)即推邊型(pushing border pattern,PBP)和彌漫浸潤型(diffuse infiltration pattern,DIP)的關系,為深入理解宮頸SCC的浸潤機制提供了新依據(jù)。方法購置2張美國Biomax公司宮頸組織芯片,每個組織點直徑1.5mm、厚度5微米,其中可用病例為109例宮頸SCC和45例宮頸非癌組織,將109例宮頸SCC按浸潤方式分為推邊型(PBP)和彌漫浸潤型(DIP)。用免疫組織化學方法檢測組織芯片的CD163陽性細胞并分別計數(shù)。結果(1)宮頸SCC癌巢組織中的M2細胞數(shù)為(35.8±28.3),宮頸非癌組織上皮的M2細胞數(shù)為(1.2±3.6),兩組之間具有非常顯著的統(tǒng)計學差異(P=0.000);(2)宮頸SCC間質中M2細胞數(shù)為(44.7±29.4),宮頸非癌組織間質的M2細胞數(shù)為(10.4±9.2),兩組之間具有非常顯著的統(tǒng)計學差異(P=0.000);(3)宮頸SCC的兩種浸潤分型中,PBP癌巢的M2細胞數(shù)為(20.5±19.5),與DIP癌巢的M2細胞數(shù)(37.7±28.7)相比,具有顯著的統(tǒng)計學差異(P=0.046);(4)宮頸SCC的兩種浸潤分型中,宮頸SCCPBP間質的M2細胞數(shù)為(25.1±18.0),與DIP癌間質的M2細胞數(shù)(47.1±29.7)相比,具有非常顯著的統(tǒng)計學差異(P=0.002)。結論(1)首次發(fā)現(xiàn),宮頸SCC癌巢及間質中的腫瘤相關巨噬細胞M2數(shù)與非癌組織相比具有非常顯著的統(tǒng)計學差異;(2)首次發(fā)現(xiàn),DIP宮頸SCC的癌巢及間質中的腫瘤相關巨噬細胞M2數(shù)顯著多于PBP宮頸SCC。這一發(fā)現(xiàn)不僅為認識宮頸SCC的浸潤機制提供了新依據(jù),而且為宮頸SCC的免疫治療提供了實驗依據(jù)。
[Abstract]:Objective Cervical cancer CCC is one of the most common cancers in women, which is the second most common cancer in the world, the third most common cancer mortality in women, and the second most common cause of cancer death in developing countries. Among them, squamous cell carcinoma of cervix squamous cell carcinoma (SCC) accounts for about 75-80% of cervical carcinoma. The invasive mode and degree of cervical cancer largely determine its prognosis and treatment. In view of the high proportion of cervical SCC and great harm, it is urgent to further understand its biological characteristics and pathogenesis, and provide scientific basis for new treatment methods. Immunohistochemical studies of various human tumor tissues show that the majority of tumor patients with high M2 count of tumor-associated macrophages have poor clinical prognosis. Therefore, the study of tumor-associated macrophages in tumor microenvironments should pay special attention to M _ 2. In this study, we first studied the relationship between tumor associated macrophage M2 (M 2) and cervical SCC invasion pattern patterns (P P) and diffusely infiltrated infiltration pattern border (DIP), which provided a new basis for further understanding the infiltration mechanism of cervical SCC. Methods two pieces of cervical tissue microarray of Biomax were purchased. Each tissue point was 1.5mm in diameter and 5 microns in thickness. The available cases were 109 cases of cervical SCC and 45 cases of non-cancerous cervical tissues. According to the invasive pattern, 109 cases of cervical SCC were divided into edge-pushing type and diffuse infiltrating type. CD163 positive cells in tissue microarray were detected by immunohistochemical method and counted separately. Results (1) the number of M2 cells in cervical SCC cancer nest tissue was 35.8 鹵28.3 and that in cervical non-cancerous epithelium was 1.2 鹵3.6. There was a very significant statistical difference between the two groups (P < 0. 000) the number of M 2 cells in cervical SCC stroma was 44.7 鹵29.44.The number of M 2 cells in cervical noncancerous tissue was 44.7 鹵29.42a. There was significant statistical difference between the two groups (P < 0. 000). In the two invasive types of cervical SCC, the number of M 2 cells was 20. 5 鹵19. 5 and 37.7 鹵28. 7 in the cancer nest of DIP. There were significant statistical differences in the number of M2 cells in cervical SCCPBP stroma between two types of cervical SCC (P < 0.01 鹵18.0), and there was a very significant statistical difference in the number of M 2 cells between DIP and DIP stroma (47.1 鹵29.7). Conclusion 1) first found, The number of tumor-associated macrophages M2 in cervical SCC nests and stroma was significantly higher than that in non-cancerous tissues. (2) it was the first time to find that the number of tumor-associated macrophages M2 in dip cervical SCC and stroma was significantly higher than that in PBP cervix SCC. This discovery not only provides a new basis for understanding the infiltrating mechanism of cervical SCC, but also provides experimental evidence for the immunotherapy of cervical SCC.
【學位授予單位】：寧夏醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R737.33
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本文編號：1909702