本文選題：正交設(shè)計(jì) + 姜黃素　； 參考：《現(xiàn)代婦產(chǎn)科進(jìn)展》2017年11期

【摘要】：目的:采用正交實(shí)驗(yàn)設(shè)計(jì),探討姜黃素作為光敏劑介導(dǎo)的光動(dòng)力治療誘導(dǎo)宮頸癌Me180細(xì)胞凋亡的最佳作用參數(shù)。方法:選定光敏劑姜黃素的藥物濃度、激光劑量、照射時(shí)間、孵育時(shí)間四個(gè)因素,分別按姜黃素濃度2.5、5、10μmol/L,激光劑量50、100、200J/cm~2,照射時(shí)間90、180、360s,孵育時(shí)間12、24、36h。每因素3個(gè)水平設(shè)計(jì)正交設(shè)計(jì)表,按正交設(shè)計(jì)表進(jìn)行姜黃素作為光敏劑介導(dǎo)的光動(dòng)力治療。治療后細(xì)胞繼續(xù)培養(yǎng),MTT法測(cè)定細(xì)胞生存率。通過雙因素方差分析三個(gè)水平的姜黃素劑量和激光劑量的估計(jì)邊緣平均值,尋找最佳治療參數(shù)。結(jié)果:對(duì)細(xì)胞凋亡的影響因素:姜黃素劑量孵育時(shí)間照射時(shí)間激光劑量;各因素水平影響:姜黃素濃度:10μmol/L5μmol/L2.5μmol/L;激光劑量:200J/cm~2100J/cm~250J/cm~2;照射時(shí)間:360s180s90s;孵育時(shí)間:24h12h36h。雙因素方差分析表明,姜黃素濃度2.5μmol/L、5μmol/L、10μmol/L三組之間兩兩比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);100J/cm~2、200J/cm~2與50J/cm~2組間比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05),但100J/cm~2與200J/cm~2組間比較差異無統(tǒng)計(jì)學(xué)意義。結(jié)論:姜黃素介導(dǎo)光動(dòng)力治療誘導(dǎo)宮頸癌細(xì)胞凋亡最佳參數(shù)組合為姜黃素濃度5μmol/L,激光劑量100J/cm~2,照射時(shí)間180s,孵育時(shí)間24h。
[Abstract]:Aim: to investigate the optimal parameters of curcumin induced apoptosis in Me180 cells of cervical cancer induced by photodynamic therapy with Guang Min by orthogonal design. Methods: the four factors of curcumin concentration, laser dose, irradiation time and incubation time were selected according to the concentration of curcumin (2.5 渭 mol / L), laser dose (50100J / cm ~ (-2), irradiation time (90180360s) and incubation time (12 ~ 24 ~ 36h). According to the orthogonal design table, curcumin was used as a Guang Min agent for photodynamic therapy. Cell survival rate was determined by MTT assay. The best treatment parameters were found by using the estimated marginal mean of curcumin dose and laser dose at three levels of ANOVA. Results: the factors affecting cell apoptosis were as follows: irradiation time of curcumin dose, laser dose, curcumin concentration: 10 渭 mol/L5 渭 mol/L2.5 渭 mol / L; laser dose: 200 J / cm ~ 2100J 路cm ~ (-1) / cm ~ (2); irradiation time: 360s ~ 180s ~ (90 s); incubating time: 24 h ~ 12 h ~ (36 h). Two-factor ANOVA analysis showed that curcumin concentration of 2.5 渭 mol / L ~ 5 渭 mol / L ~ (10) 渭 mol / L ~ (10 渭 mol/L) was significantly higher than that of 50J/cm~2 group (P 0.05), but there was no significant difference between 100J/cm~2 group and 200J/cm~2 group. Conclusion: the best parameters for inducing apoptosis of cervical cancer cells induced by curcumin mediated photodynamic therapy are curcumin concentration of 5 渭 mol / L, laser dose of 100 J / cm ~ (-2), irradiation time of 180 s and incubation time of 24 h.
【作者單位】： 中日友好醫(yī)院婦產(chǎn)科;中日友好醫(yī)院臨床研究所分子生物學(xué)室;北京大學(xué)中日友好臨床醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金(No:81202966)
【分類號(hào)】：R737.33

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