本文選題：外陰陰道念珠菌病 + 白念珠菌��； 參考：《中國感染與化療雜志》2017年03期

【摘要】：目的探討上海地區(qū)外陰陰道念珠菌病的菌種分布及其主要致病菌的分子流行特點(diǎn),并進(jìn)行菌株遺傳進(jìn)化分析。方法收集2015年8月至2016年2月復(fù)旦大學(xué)附屬婦產(chǎn)科醫(yī)院、上海市第一婦嬰保健院及國際和平婦幼保健院3所醫(yī)院外陰陰道念珠菌病患者分離得念珠菌共2 185株,進(jìn)行菌種鑒定及統(tǒng)計分析,并采用ATB FUNGUS 3試劑盒檢測抗真菌藥物體外最低抑菌濃度,對其主要致病菌運(yùn)用多位點(diǎn)序列分型(MLST)的方法進(jìn)行分子分型,同時應(yīng)用e BURST和MEGA6.0軟件進(jìn)行遺傳進(jìn)化分析。結(jié)果 2 185株念珠菌屬中共鑒定出白念珠菌1 988株,光滑念珠菌149株,熱帶念珠菌20株,其他念珠菌28株;白念珠菌對氟康唑耐藥率為6.5%;93株白念珠菌共檢測出26個二倍體序列型(DST),其中50株敏感菌序列型為分散分布,而43株耐氟康唑白念珠菌集中于同一個克隆復(fù)合體(CC 69),并處于同一個進(jìn)化樹分枝上(Clade 1)。結(jié)論白念珠菌是上海地區(qū)3所婦產(chǎn)科醫(yī)院外陰陰道念珠菌病的主要致病菌,對三唑類藥物的耐藥率較高,而且耐氟康唑白念珠菌表現(xiàn)為CC 69的克隆傳播流行,需要進(jìn)行重點(diǎn)監(jiān)控。
[Abstract]:Objective to investigate the distribution of vulvovaginal candidiasis and the molecular epidemiological characteristics of the main pathogenic bacteria in Shanghai, and to analyze the genetic evolution of the strains. Methods from August 2015 to February 2016, 2 185 strains of candidiasis were isolated from patients with vulvovaginal candidiasis in the affiliated Obstetrics and Gynecology Hospital of Fudan University, Shanghai first Maternal and Child Health Hospital and International Peace Maternal and Child Health Hospital. The ATB FUNGUS 3 kit was used to detect the minimal inhibitory concentration of antifungal drugs in vitro, and the multilocus sequence typing method was used to type the main pathogenic bacteria. At the same time, the genetic evolution analysis was carried out by using e BURST and MEGA6.0 software. Results A total of 1 988 Candida albicans, 149 Candida lucidum, 20 Candida tropics and 28 other Candida strains were identified from 2 185 Candida strains. The resistance rate of Candida albicans to fluconazole was 6.5% and 93 strains of Candida albicans were detected. 43 strains of fluconazole-resistant Candida albicans were concentrated in the same clone complex CC69, and were located on the same branch of the evolutionary tree. Conclusion Candida albicans is the main pathogen of vulvovaginal candidiasis in 3 obstetrics and gynecology hospitals in Shanghai, and the resistance rate to triazole is high, and fluconazole-resistant Candida albicans is the clone transmission epidemic of CC69. Key monitoring needs to be carried out.
【作者單位】： 復(fù)旦大學(xué)附屬婦產(chǎn)科醫(yī)院檢驗科;上海市第一婦嬰保健院檢驗科;國際和平婦幼保健院檢驗科;
【基金】：上海申康醫(yī)院發(fā)展中心市級醫(yī)院臨床輔助科室能力建設(shè)項目(SHDC22014016)
【分類號】：R711.31

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本文編號：1881076