發(fā)布時間：2018-05-03 12:55

  本文選題：子宮腔粘連 + ER　； 參考：《臨床與實驗病理學(xué)雜志》2015年04期

【摘要】：目的探討雌激素受體(ER)、孕激素受體(PR)在子宮腔粘連(intrauterine adheious,IUA)患者子宮內(nèi)膜組織中的表達(dá)及其臨床意義。方法采用免疫組化Max Vision兩步法和實時熒光定量PCR(qRT-PCR)技術(shù)檢測IUA組(研究組)和非IUA組(對照組)子宮內(nèi)膜組織中ER、PR的表達(dá)水平。結(jié)果免疫組化檢測顯示ER蛋白在研究組(3.52±0.71)和對照組(2.75±1.00)中的表達(dá)差異有統(tǒng)計學(xué)意義(P=0.01);qRT-PCR法檢測顯示ER mRNA在研究組(1.59±0.26)和對照組(1.00±0.19)中的表達(dá)差異有統(tǒng)計學(xué)意義(P=0.00)。免疫組化檢測顯示PR蛋白在研究組(3.26±0.70)和對照組(3.58±0.28)中的表達(dá)差異無統(tǒng)計學(xué)意義(P=0.12);qRT-PCR法檢測顯示PR mRNA在研究組(1.15±0.21)和對照組(1.00±0.31)中的表達(dá)差異無統(tǒng)計學(xué)意義(P=0.21)。免疫組化檢測顯示ER、PR蛋白分別在子宮內(nèi)膜腺體和子宮內(nèi)膜間質(zhì)中均有表達(dá),差異有統(tǒng)計學(xué)意義(χ2=5.797,P=0.016;χ2=4.857,P=0.027)。結(jié)論子宮內(nèi)膜組織中ER表達(dá)研究組高于對照組,PR在兩組之間的表達(dá)無差異;ER、PR蛋白在兩組子宮內(nèi)膜腺體中表達(dá)較多,在子宮內(nèi)膜間質(zhì)中表達(dá)較少甚至不表達(dá);ER、PR表達(dá)的特點為IUA患者臨床使用雌、孕激素治療提供一定的理論參考。
[Abstract]:Objective to investigate the expression and clinical significance of estrogen receptor ERA and progesterone receptor PRA in endometrial tissue of patients with intrauterine adhesion (IUAA). Methods the expression of ERPR in endometrium of IUA group (study group) and non- group (control group) was detected by immunohistochemical Max Vision two-step method and real-time fluorescence quantitative PCRQ RT-PCR technique. Results the expression of ER protein in the study group (3.52 鹵0.71) was significantly different from that in the control group (2.75 鹵1.00). The expression of ER mRNA in the study group (1.59 鹵0.26) and the control group (1.00 鹵0.19) was significantly different by RT-PCR. The expression of PR protein in the study group (3.26 鹵0.70) and the control group (3.58 鹵0.28) was not significantly different from that in the control group (P < 0.01). The expression of PR mRNA in the study group (1.15 鹵0.21) and the control group (1.00 鹵0.31) had no significant difference (P0.21). The expression of ERP protein in endometrial glands and endometrial stroma was detected by immunohistochemistry, and the difference was statistically significant (蠂 2 / 5.797 / P ~ (0.016); 蠂 ~ (2) = 4.857 / P ~ (0.027). Conclusion the expression of ER in endometrial tissue in study group is higher than that in control group. There is no difference in the expression of ER and PR between the two groups. The characteristics of low or no expression of ERP PR in the stroma of endometrium provide a theoretical reference for the clinical use of estrogen and progesterone in patients with IUA.
【作者單位】： 安徽醫(yī)科大學(xué)第二附屬醫(yī)院婦產(chǎn)科;
【基金】：國家自然科學(xué)基金青年科學(xué)基金(81100412)
【分類號】：R711.74

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本文編號：1838569