本文選題：人乳頭瘤病毒 + 基因型�。� 參考：《中華腫瘤防治雜志》2015年19期

【摘要】：目的分析武漢市第五醫(yī)院篩查的女性生殖道人乳頭瘤病毒(human papillomavirus,HPV)的感染狀況以及其分布特點(diǎn),為女性宮頸癌的防治及HPV疫苗研制提供流行病學(xué)依據(jù)。方法收集2011-05-01-2014-09-30武漢市第五醫(yī)院體檢或婦科就診的女性宮頸脫落細(xì)胞樣本共2 409份,采用導(dǎo)流雜交基因芯片技術(shù)對(duì)樣本進(jìn)行HPV基因分型檢測(cè),檢測(cè)HPV16、18、31、33、35、39、45、51、52、53、56、58、59、66和68共15種高危亞型和HPV6、11、42、43、44和81共6種低危亞型。按年齡將研究對(duì)象分為5組,18~30歲、30~40歲、40~50歲、50~60歲和60歲組,并對(duì)HPV感染病例的年齡分布以及基因型進(jìn)行分析。結(jié)果共檢測(cè)樣本2 409例,檢出HPV陽(yáng)性樣本422例,HPV陽(yáng)性檢出率為17.51%。21種HPV亞型中共檢出20種亞型,低危型HPV43未檢出,其中HPV高危亞型約占82.76%。HPV高危亞型按檢出頻次由高到低依次為16(19.24%)、52(15.63%)、58(10.42%)、53(6.61%)、33(5.61%)、39(5.01%)、18(4.81%)、31(4.01%)、68(3.41%)、66(2.61%)、59(2.20%)、56(1.40%)、51(1.00%)、35(0.60%)和45(0.20%),HPV低危亞型由高到低依次為81(8.22%)、11(5.21%)、6(2.40%)、44(1.00%)和42(0.40%)。感染類型以單一亞型感染為主,約占83.64%,其中高危亞型檢出率達(dá)到83.56%。單一亞型感染病例中的高危亞型檢出率低于其他2種感染類型,P0.05。50~60歲人群的陽(yáng)性檢出率(22.57%)高于其他年齡組的陽(yáng)性檢出率,P0.05。40~50歲人群HPV感染優(yōu)勢(shì)亞型首位為HPV52,不同于其余各年齡段人群HPV感染的首位優(yōu)勢(shì)亞型(HPV16)。結(jié)論武漢市第五醫(yī)院篩查的女性生殖道HPV感染的主要亞型為高危亞型HPV16、52。感染類型以單一亞型感染為主,其中高危亞型檢出率最高。50~60歲人群的HPV陽(yáng)性檢出率高于其他年齡分組,臨床應(yīng)重視該年齡段人群的HPV篩查。
[Abstract]:Objective to analyze the status and distribution of human papillomavirus (HPV) infection in female genital tract human papillomavirus (HPV) screened in the Fifth Hospital of Wuhan, and to provide epidemiological evidence for the prevention and treatment of female cervical cancer and the development of HPV vaccine. Methods A total of 2 409 samples of female cervical exfoliated cells were collected from the fifth hospital of Wuhan from 2011-05-01-2014-09-30. The HPV genotyping was detected by using diversion hybridization gene chip technique. A total of 15 high risk subtypes of HPV16, HPV6112A42O43M44 and 81 were detected for HPV16 (18 / 31), 3' 3' / 3' / 3' / 451 / 551, 51n / 52N, 52N, 58N / 596, 68, and HPV6111142A43M44 and 81, respectively, in which 6 low risk subtypes were detected. The subjects were divided into 5 groups according to age. The age distribution and genotype of HPV infection cases were analyzed. Results A total of 2 409 cases were detected. The positive rate of HPV in 422 cases was 17.51%. 20 subtypes were detected in 21 HPV subtypes, but 20 subtypes were not detected in low-risk HPV43. 鍏朵腑HPV楂樺嵄浜氬瀷綰﹀崰82.76%.HPV楂樺嵄浜氬瀷鎸夋鍑洪嬈＄敱楂樺埌浣庝緷嬈′負(fù)16(19.24%),52(15.63%),58(10.42%),53(6.61%),33(5.61%),39(5.01%),18(4.81%),31(4.01%),68(3.41%),66(2.61%),59(2.20%),56(1.40%),51(1.00%),35(0.60%)鍜，

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