發(fā)布時(shí)間：2018-04-20 23:21

  本文選題：絨癌 + F基因��； 參考：《南方醫(yī)科大學(xué)學(xué)報(bào)》2015年05期

【摘要】：目的探討葡萄胎病理相關(guān)新基因F10與絨癌細(xì)胞系JEG-3裸鼠皮下成瘤的關(guān)系。方法通過(guò)細(xì)胞轉(zhuǎn)染技術(shù)及RNA干擾技術(shù),分別建立和篩選出F10基因穩(wěn)定過(guò)表達(dá)及沉默的絨癌細(xì)胞系JEG-3。取SPF級(jí)裸鼠(4~5周齡)30只,隨機(jī)均分為JEG-3F10過(guò)表達(dá)組、JEG-3 F10沉默組、JEG-3未處理組(n=10),分別接種F10基因過(guò)表達(dá)的JEG-3細(xì)胞、F10基因沉默的JEG-3細(xì)胞和未處理的JEG-3細(xì)胞株5×107個(gè)于頸背部皮下。接種后每3~4 d稱量裸鼠質(zhì)量觀察,記錄腫瘤發(fā)生時(shí)間,觀察皮下腫瘤的生長(zhǎng)情況。繪制在體腫瘤生長(zhǎng)曲線,計(jì)算各組裸鼠的成瘤率。結(jié)果 3組的成瘤率均為100%(10/10)。JEG-3 F10過(guò)表達(dá)組、JEG-3F10沉默組、JEG-3未處理組的成瘤時(shí)間分別為6.2±0.78、7±2.49、6.3±0.67 d,組間比較無(wú)統(tǒng)計(jì)學(xué)差異(F=0.781,P=0.468)。JEG-3 F10過(guò)表達(dá)組腫瘤的在體生長(zhǎng)速度較JEG-3 F10沉默組、JEG-3未處理組增快,差異有統(tǒng)計(jì)學(xué)意義(P0.05),JEG-3 F10未處理組腫瘤的在體生長(zhǎng)速度較JEG-3 F10沉默組快(P0.05)。細(xì)胞接種5周后處死小鼠,取瘤組織稱重,JEG-3 F10過(guò)表達(dá)組、JEG-3 F10沉默組、JEG-3未處理組腫瘤重量分別為571.1±221.10 mg、136.2±66.25 mg、354.5±116.23 mg,組間比較存在統(tǒng)計(jì)學(xué)差異(F=21.199,P=0.000)。結(jié)論 F10基因與絨癌細(xì)胞系JEG-3的增殖調(diào)節(jié)有關(guān),可增強(qiáng)JEG-3細(xì)胞系在裸鼠體內(nèi)的致瘤性。
[Abstract]:Objective to investigate the relationship between a novel histopathological gene F10 and subcutaneous tumorigenesis of choriocarcinoma cell line JEG-3 in nude mice. Methods A choriocarcinoma cell line JEG-3 with stable overexpression and silencing of F10 gene was established and screened by cell transfection and RNA interference. Thirty SPF grade nude mice were randomly divided into JEG-3F10 overexpression group (JEG-3 F10 silencing group) and JEG-3 untreated group (JEG-3 untreated group). JEG-3 cells silenced by F10 gene over-expressed JEG-3 cells and 5 脳 107 untreated JEG-3 cell lines were inoculated subcutaneously on the neck and back respectively. The quality of nude mice was measured every 3 ~ 4 days after inoculation, the occurrence time of tumor was recorded and the growth of subcutaneous tumor was observed. The tumor growth curve in vivo was drawn and the tumorigenic rate of each group was calculated. Results the tumorigenesis rate of the three groups was 6.2 鹵0.787 鹵2.49 鹵0.67 days in the 100%(10/10).JEG-3 F10 overexpression group and JEG-3F10 silencing group, respectively. There was no significant difference between the three groups. The growth rate of the tumor in the overexpression group was faster than that in the untreated group of JEG-3 F10 silencing group. The in vivo growth rate of JEG-3 F10 untreated group was faster than that of JEG-3 F10 silencing group (P 0.05). After 5 weeks of cell inoculation, the mice were killed. The tumor weight of the untreated JEG-3 F10 group was 571.1 鹵221.10 mg / g, respectively, and the tumor weight was 571.1 鹵221.10 mg / kg, 354.5 鹵116.23 mg / g respectively. There was a significant difference between the two groups in F21.199P0.000mg. Conclusion F10 gene is related to the proliferation regulation of choriocarcinoma cell line JEG-3 and can enhance the tumorigenicity of JEG-3 cell line in nude mice.
【作者單位】： 南方醫(yī)科大學(xué)南方醫(yī)院婦產(chǎn)科;
【基金】：國(guó)家自然科學(xué)基金(81170595)~~
【分類號(hào)】：R737.33

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本文編號(hào)：1779906