本文選題：多囊卵巢綜合癥 + 結(jié)合珠蛋白��； 參考：《重慶醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:探討多囊卵巢綜合癥(PCOS)患者血清結(jié)合珠蛋白(Hp)水平及其與各代謝指標(biāo)的相關(guān)性;并進一步探討PCOS患者Hp基因型和基因頻率的分布及其與PCOS之間的關(guān)系。第一部分:研究對象:PCOS組選擇符合PCOS診斷標(biāo)準(zhǔn)的PCOS患者106例,對照組選擇無高雄激素血癥、月經(jīng)規(guī)律的不孕癥婦女86例為研究對象。方法:測定身高、體重,計算體重指數(shù)(BMI),根據(jù)亞洲人群中正常體重指數(shù)指標(biāo),將PCOS組分為非肥胖PCOS組(57例)和肥胖PCOS組(49例);行口服葡萄糖耐量試驗和胰島素釋放試驗,計算穩(wěn)態(tài)模型胰島素抵抗指數(shù)(HOMA-IR),按照胰島素抵抗(IR)診斷標(biāo)準(zhǔn),將PCOS組分為胰島素抵抗PCOS組(45例)和非胰島素抵抗PCOS組(61例)。酶聯(lián)免疫吸附法(ELISA)檢測所有研究對象血清Hp水平。結(jié)果:(1)PCOS組血清Hp水平顯著高于對照組(P0.01),(2)肥胖PCOS組血清Hp水平高于非肥胖PCOS組(P0.01);(3)胰島素抵抗PCOS組血清Hp水平顯著高于非胰島素抵抗PCOS組(P0.01)。(4)血清Hp水平變化與BMI(P0.01)、HOMA-IR(P0.01)正相關(guān)結(jié)論:PCOS患者血清Hp水平與BMI、HOMA-IR正相關(guān),提示Hp可能與PCOS患者的肥胖和胰島素抵抗關(guān)系密切。結(jié)合珠蛋白可能是PCOS診治過程中的有效觀察指標(biāo)。第二部分:研究對象:PCOS組選擇符合PCOS診斷標(biāo)準(zhǔn)的PCOS患者106例,對照組選擇無高雄激素血癥、月經(jīng)規(guī)律的不孕癥婦女86例為研究對象。方法:PCR法檢測Hp基因型和基因頻率的分布。結(jié)果:PCOS組與對照組Hp2-2基因型頻率為0.368和0.186;Hp2-1基因型頻率為0.424和0.477;Hp1-1基因型頻率為0.208和0.337。PCOS組Hp2-2基因型頻率顯著高于對照組(0.368vs0.186,P0.01),Hp2等位基因頻率也高于對照組(0.580vs0.424,P0.01)。結(jié)論:Hp2基因在PCOS中有聚集現(xiàn)象,攜有Hp2-2基因型的人群可能對PCOS有較高的遺傳易感性。
[Abstract]:Objective: to investigate the relationship between serum globin (Hp) level and metabolic indexes in patients with polycystic ovary syndrome (PCOS), and to explore the distribution of HP genotype and gene frequency in patients with PCOS and their relationship with PCOS.Part one: the subjects of the study were 106 PCOS patients who met the diagnostic criteria of PCOS and 86 infertile women who had no hyperandrogenemia and regular menstruation in the control group.Methods: height, body weight, body mass index (BMI) were measured. According to the normal body mass index (BMI) in Asian population, PCOS was divided into non-obese PCOS group (n = 57) and obese PCOS group (n = 49). Oral glucose tolerance test (OGTT) and insulin release test were performed.According to the diagnostic criteria of insulin resistance, PCOS was divided into insulin resistance (PCOS) group (n = 45) and non-insulin resistance (PCOS) group (n = 61).Serum HP levels in all subjects were detected by enzyme linked immunosorbent assay (Elisa).Conclusion Serum HP level in patients with PCOS is positively correlated with BMIOHOMA-IR.The results suggest that HP may be associated with obesity and insulin resistance in PCOS patients.Binding globin may be an effective index in the diagnosis and treatment of PCOS.Part two: one hundred and six PCOS patients who met the diagnostic criteria of PCOS were selected in the study group. 86 infertile women without hyperandrogenemia and regular menstruation were selected as the subjects in the control group.Methods HP genotypes and gene frequencies were detected by PCR.Results the frequencies of Hp2-2 genotypes 0.368 and 0.186 Hp2-1 were 0.424 and 0.477Hp1-1, and the frequencies of Hp2-2 genotype in 0.337.PCOS group were significantly higher than those in control group (0.368vs0.186 vs 0.186 P0.01P0.01a). The frequencies of Hp2 allele were also higher than those of control group (0.580vs0.424P0.01a).Conclusion there is a clustering phenomenon in PCOS with the gene of 1: Hp2, and the population with Hp2-2 genotype may have a higher genetic susceptibility to PCOS.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R711.75

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