本文選題：卵泡膜細(xì)胞　切入點(diǎn)：氧化型低密度脂蛋白　出處：《上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年03期

【摘要】：目的·初步探討ox-LDL對(duì)大鼠卵泡膜細(xì)胞增殖和雄激素合成相關(guān)基因LXR-α和St AR表達(dá)的影響。方法·免疫組化檢測(cè)大鼠卵巢組織中LXR-α的表達(dá)。體外分離培養(yǎng)原代大鼠卵泡膜細(xì)胞,分別用25、50、100、150、200、300和400 mg/L的ox-LDL處理,用real-time PCR檢測(cè)LXR-αm RNA的變化,用MTT檢測(cè)細(xì)胞活力,用Western blotting檢測(cè)LXR-α和St AR蛋白的表達(dá)。結(jié)果·ox-LDL對(duì)大鼠卵泡膜細(xì)胞增殖的影響和對(duì)LXR-α和St AR表達(dá)的調(diào)控呈現(xiàn)濃度依賴性變化。ox-LDL刺激24 h后,低濃度的ox-LDL(25~150 mg/L)可誘導(dǎo)卵泡膜細(xì)胞增殖,以100 mg/L的ox-LDL對(duì)卵泡膜細(xì)胞增殖的促進(jìn)作用顯著。而當(dāng)ox-LDL濃度繼續(xù)上升,細(xì)胞存活率下降,以400 mg/L的ox-LDL對(duì)卵泡膜細(xì)胞增殖的抑制作用顯著。低濃度ox-LDL(25~150 mg/L)刺激,導(dǎo)致LXR-αm RNA的表達(dá)升高,其中150 mg/L ox-LDL對(duì)LXR-αm RNA的表達(dá)量影響顯著。高濃度ox-LDL抑制LXR-αm RNA的表達(dá),其中400 mg/L的ox-LDL對(duì)LXR-αm RNA的表達(dá)量影響顯著。150 mg/L ox-LDL促進(jìn)大鼠卵泡膜細(xì)胞LXR-α和St AR蛋白的表達(dá)上升,但150 mg/L ox-LDL對(duì)St AR蛋白表達(dá)的促進(jìn)作用,與對(duì)照組相比,差異沒有統(tǒng)計(jì)學(xué)意義;而400 mg/L ox-LDL能顯著抑制大鼠卵泡膜細(xì)胞LXR-α和St AR蛋白的表達(dá)。結(jié)論·低濃度的ox-LDL可誘導(dǎo)卵泡膜細(xì)胞增殖,促進(jìn)LXR-α和St AR的表達(dá),而高濃度oxLDL降低細(xì)胞活力,抑制LXR-α和St AR的表達(dá)。
[Abstract]:Objective to investigate the effects of ox-LDL on proliferation and androgen synthesis related genes LXR- 偽 and St AR expression in rat follicular membrane cells.Methods the expression of LXR- 偽 in rat ovary was detected by immunohistochemistry.Primary rat follicular membrane cells were isolated and cultured in vitro. The cells were treated with 2550100150200300 and 400 mg/L ox-LDL respectively. The changes of LXR- 偽 m RNA were detected by real-time PCR, the cell viability was detected by MTT, and the expression of LXR- 偽 and St AR proteins were detected by Western blotting.Results the effects of ox-LDL on the proliferation of rat follicular membrane cells and the regulation of LXR- 偽 and St AR expression showed concentration-dependent changes. After 24 h of stimulation with ox-LDL, low concentration of ox-LDL(25~150 mg / L could induce the proliferation of follicular membrane cells.The effect of 100 mg/L ox-LDL on the proliferation of follicular membrane cells was significant.However, when the concentration of ox-LDL continued to increase, the cell survival rate decreased, and the inhibitory effect of 400 mg/L ox-LDL on the proliferation of follicular membrane cells was significant.Low concentration of ox-LDL(25~150 mg / L) stimulated the expression of LXR- 偽 m RNA, and 150 mg/L ox-LDL significantly affected the expression of LXR- 偽 m RNA.400 mg/L ox-LDL could significantly inhibit the expression of LXR- 偽 and St AR protein in rat follicular membrane cells.Conclusion low concentration of ox-LDL can induce the proliferation of follicular membrane cells and promote the expression of LXR- 偽 and St AR, while high concentration of oxLDL can decrease cell viability and inhibit the expression of LXR- 偽 and St AR.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院婦產(chǎn)科;重慶市人口和計(jì)劃生育科學(xué)技術(shù)研究院國家衛(wèi)生計(jì)生委出生缺陷與生殖健康重點(diǎn)實(shí)驗(yàn)室;
【基金】：重慶市衛(wèi)生和計(jì)劃生育委員會(huì)2011年醫(yī)學(xué)科研計(jì)劃項(xiàng)目(2011-2-104)~~
【分類號(hào)】：R711.75

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