本文選題：隱匿性乙型肝炎病毒感染　切入點：顯性乙型肝炎病毒感染　出處：《中國公共衛(wèi)生》2016年11期

【摘要】：目的探討孕婦人群顯性和隱匿性乙型肝炎病毒(HBV)感染及其HBV病毒株S基因的分子進化特征。方法 2014年6月1日—31日,在知情同意下,連續(xù)招募安徽省安慶市立醫(yī)院產(chǎn)科初次住院活胎孕婦156人,收集其人口學(xué)特征和血清;對所有血清平行進行乙型肝炎病毒表面抗原(HBsAg)酶聯(lián)免疫吸附法和電化學(xué)發(fā)光法檢測、應(yīng)用實時PCR定量測定HBV DNA、應(yīng)用巢式PCR進行HBV S片段擴增、測序及進化分析。結(jié)果156名孕婦中,顯性和隱匿性HBV感染率分別為10.3%(16/156)、8.6%(12/140);成功獲得13株HBV S基因序列,其中顯性7株,隱匿性6株;所有病毒株均為B基因型;僅1例隱匿性HBV株為ayw1血清型,其余12株均為adw2血清型。結(jié)論孕婦人群攜帶較高比例的顯性和隱匿性HBV。
[Abstract]:Objective to investigate the dominant and occult hepatitis B virus (HBV) infection in pregnant women and the molecular evolution of S gene of HBV virus strain.Methods from June 1 to 31, 2014, 156 pregnant women were recruited from Anhui Anqing Municipal Hospital for the first time in obstetrics department with informed consent to collect their demographic characteristics and serum.All sera were detected by enzyme linked immunosorbent assay (Elisa) and electrochemiluminescence assay (ECL) for hepatitis B virus surface antigen HBsAg (HBsAg), real-time PCR was used to quantify HBV, nested PCR was used to amplify HBV S fragment, sequencing and evolution analysis were performed.Results among 156 pregnant women, the dominant and occult HBV infection rates were 10.3 / 156 / 8.66 / 140, respectively, and 13 HBV S gene sequences were successfully obtained, of which 7 were dominant and 6 were occult. All the virus strains were B genotypes, and only one occult HBV strain was ayw1 serotype.The other 12 strains were all adw2 serotypes.Conclusion pregnant women carry a high proportion of dominant and occult HBV.
【作者單位】： 復(fù)旦大學(xué)公共衛(wèi)生學(xué)院衛(wèi)生微生物學(xué)教研室;復(fù)旦大學(xué)公共衛(wèi)生學(xué)院教育部公共衛(wèi)生重點實驗室;復(fù)旦大學(xué)衛(wèi)生部衛(wèi)生技術(shù)評估重點實驗室;安徽省安慶市立醫(yī)院檢驗科;安徽省安慶市立醫(yī)院婦產(chǎn)科;復(fù)旦大學(xué)公共衛(wèi)生學(xué)院衛(wèi)生統(tǒng)計學(xué)教研室;
【基金】：國家自然科學(xué)基金(81373065) 上海市教育委員會科研創(chuàng)新重點項目(14ZZ015)
【分類號】：R714.251
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本文編號：1712039