發(fā)布時(shí)間：2018-04-04 17:37

  本文選題：原因不明復(fù)發(fā)性流產(chǎn)　切入點(diǎn)：Th17細(xì)胞　出處：《廣西醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：通過檢測(cè)原因不明復(fù)發(fā)性流產(chǎn)（URSA）患者外周血和蛻膜組織中Th17、Th22細(xì)胞比例及白細(xì)胞介素-22（IL-22）水平，了解URSA患者外周血及蛻膜組織中Th17、Th22細(xì)胞比例及IL-22水平變化。 方法：選取2013年9月至2014年2月于廣西醫(yī)科大學(xué)第一附屬醫(yī)院就診的原因不明復(fù)發(fā)性流產(chǎn)患者15例為研究組，，正常早孕要求人流（NP）的患者為對(duì)照組。于人流術(shù)前采集其肘靜脈血5ml，人流術(shù)后留取蛻膜組織。通過流式細(xì)胞術(shù)和酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）檢測(cè)其外周血及蛻膜組織中Th17、Th22細(xì)胞比例及IL-22水平。 結(jié)果：1、URSA組患者外周血及蛻膜組織Th17（CD4+IL-17+）細(xì)胞比例分別為1.62%±0.52%和2.11%±0.61%，均高于相應(yīng)NP組患者1.24%±0.27%和1.47%±0.41%，,差異有統(tǒng)計(jì)學(xué)意義（P0.05）。并且蛻膜局部Th17細(xì)胞比例水平高于外周血，差異有統(tǒng)計(jì)學(xué)意義（P0.05）。 2、 URSA組患者外周血及蛻膜組織Th22細(xì)胞比例分別為1.59%±0.52%和1.91%±0.58%，均明顯高于相應(yīng)NP組患者1.11%±0.24%和1.33%±0.32%，,差異有統(tǒng)計(jì)學(xué)意義（P0.01）。并且蛻膜局部Th22細(xì)胞比例水平高于外周血，差異有統(tǒng)計(jì)學(xué)意義（P0.05）。 3、 URSA組患者外周血及蛻膜組織中IL-22水平分別是181.98±8.10pg/ml，198.52±10.98pg/ml，均高于對(duì)應(yīng)NP組患者104.28±4.73pg/ml，110.47±6.43pg/ml，差異有統(tǒng)計(jì)學(xué)意義（P0.01）。并且蛻膜局部IL-22水平高于外周血，差異有統(tǒng)計(jì)學(xué)意義（P0.01）。 4、比較URSA組患者與NP組患者外周血及蛻膜組織中IL-22細(xì)胞因子水平與Th17細(xì)胞比例的相關(guān)性，其相關(guān)系數(shù)及P值分別為：外周血中(r=0.157, P0.05)、(r=0.437, P0.05)；蛻膜中(r=0.069, P0.05)、(r=0.235,P0.05)。結(jié)果示兩變量間線性關(guān)系極弱或無線性關(guān)系存在。 5、比較URSA組患者與NP組患者外周血及蛻膜組織中IL-22細(xì)胞因子水平與Th22細(xì)胞比例的相關(guān)性，其相關(guān)系數(shù)及P值分別為：外周血中(r=0.885,P0.05)、(r=0.749, P0.01)；蛻膜中(r=0.849, P0.05)、(r=0.934,P0.01)。結(jié)果均呈正相關(guān)關(guān)系。 6、分別比較URSA組患者與NP組患者外周血與蛻膜組織中Th17細(xì)胞比例的相關(guān)性，其相關(guān)系數(shù)及P值分別為：(r=0.661, P0.01)、(r=0.954,P0.01)均呈正相關(guān)關(guān)系。 7、分別比較URSA組患者與NP組患者外周血與蛻膜組織中Th22細(xì)胞比例的相關(guān)性，其相關(guān)系數(shù)及P值分別為：(r=0.685, P0.01)、(r=0.943,P0.01)，均呈正相關(guān)關(guān)系。 8、分別比較URSA組患者與NP組患者外周血與蛻膜組織中IL-22水平的相關(guān)性，其相關(guān)系數(shù)及P值分別為：(r=0.875, P0.01)、(r=0.823, P0.01)均呈正相關(guān)關(guān)系。 結(jié)論：1、妊娠婦女外周血及蛻膜組織中Th17、Th22細(xì)胞比例和IL-22水平增高與URSA的發(fā)生具有相關(guān)性；并且相較于外周血，局部蛻膜中Th17、Th22細(xì)胞比例和IL-22水平改變與妊娠狀態(tài)有更密切的關(guān)系。 2、妊娠婦女（包括URSA患者）外周血與局部蛻膜的Th17、Th22細(xì)胞比例及IL-22水平改變呈平行改變。 3、外周血及蛻膜組織中Th17、Th22細(xì)胞比例及IL-22水平可能可以作為URSA的預(yù)測(cè)指標(biāo)。
[Abstract]:Objective: to investigate the changes of Th17 Th22 cells and IL-22 levels in peripheral blood and decidua of patients with URSA by detecting the proportion of Th17 Th22 cells in peripheral blood and decidua tissues of patients with unexplained recurrent abortion (Ursa) and the level of interleukin-22 (IL-22).Methods: from September 2013 to February 2014, 15 patients with unexplained recurrent abortion were selected as study group, and those with normal early pregnancy as control group.The cubital venous blood was collected 5 ml before the abortion and the decidua tissue was retained after the abortion.The proportion of Th17 Th22 cells and the level of IL-22 in peripheral blood and decidua were detected by flow cytometry and Elisa.The percentage of Th17 cells in decidua was higher than that in peripheral blood, and the difference was statistically significant (P 0.05).The percentage of Th22 cells in decidua was higher than that in peripheral blood, and the difference was statistically significant (P 0.05).3. The levels of IL-22 in peripheral blood and decidua in URSA group were 181.98 鹵8.10 PG / ml, 198.52 鹵10.98 PG / ml, respectively, which were significantly higher than those in NP group (104.28 鹵4.73 PG / ml, 110.47 鹵6.43 PG / ml, P 0.01).The level of local IL-22 in decidua was higher than that in peripheral blood, and the difference was statistically significant (P 0.01).4,姣旇緝URSA緇勬?zhèn)ｈ�呬笌NP緇勬?zhèn)ｈ�呭鍛ㄨ鍙?qiáng)铚曡啘缁劸l囦腑IL-22緇嗚優(yōu)鍥犲瓙姘村鉤涓嶵h17緇嗚優(yōu)姣斾緥鐨勭浉鍏蟲

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