本文選題：培養(yǎng)模式　切入點(diǎn)：胚胎質(zhì)量　出處：《生殖與避孕》2015年01期

【摘要】：目的:比較2種胚胎培養(yǎng)模式對(duì)胚胎質(zhì)量和妊娠結(jié)局的影響。方法:收集首次行IVF-ET治療、年齡38歲、采用長(zhǎng)方案促排卵、常規(guī)IVF授精、正常受精卵數(shù)3枚且第3日有新鮮胚胎移植的518個(gè)周期的臨床資料,根據(jù)正常受精卵采用的培養(yǎng)模式分成單胚胎培養(yǎng)組(n=254)和胚胎群組培養(yǎng)組(n=264)。分析患者的基本資料、胚胎發(fā)育情況以及妊娠結(jié)局。結(jié)果:患者的年齡、不孕年限、基礎(chǔ)卵泡刺激素(FSH)、Gn用量、h CG注射日內(nèi)膜厚度以及各種不孕因素的比例等參數(shù)組間差異均無統(tǒng)計(jì)學(xué)意義(P0.05);平均獲卵數(shù)、成熟率、2PN率等指標(biāo)組間也均無統(tǒng)計(jì)學(xué)差異(P0.05),但胚胎群組培養(yǎng)組的卵裂率、第3日優(yōu)質(zhì)胚胎率、剩余胚胎可利用囊胚形成率均明顯高于單胚胎培養(yǎng)組,差異有統(tǒng)計(jì)學(xué)意義(P0.05);胚胎群組培養(yǎng)組的種植率和臨床妊娠率高于單胚胎培養(yǎng)組,但差異無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:采用胚胎群組培養(yǎng)模式可以得到更多的優(yōu)質(zhì)胚胎和更高的囊胚形成率,推薦可用于日常IVF治療周期中的胚胎培養(yǎng)。
[Abstract]:Objective: to compare the effects of two kinds of embryo culture models on embryo quality and pregnancy outcome. Methods: the first IVF-ET therapy, aged 38 years, was used to promote ovulation with long regimen and conventional IVF insemination. There were 3 normal fertilized eggs and 518 cycles of fresh embryo transfer on the 3rd day. According to the culture model of normal fertilized eggs, they were divided into single embryo culture group and embryo group culture group. The basic data of the patients were analyzed. Embryonic development and pregnancy outcome. Results: age of the patient, years of infertility, There was no significant difference in endometrial thickness and the proportion of various infertility factors between the two groups, and there was no significant difference between the two groups, and the average number of eggs obtained was higher than that of the control group. There was no significant difference in maturation rate and 2PN rate between the two groups, but the cleavage rate, the third high quality embryo rate and the blastocyst formation rate in the group of embryo group culture were significantly higher than those in the group of single embryo culture, and the rate of blastocyst formation was significantly higher in the group of embryo group culture than in the group of single embryo culture. The implantation rate and clinical pregnancy rate of embryo group culture group were higher than that of single embryo culture group. Conclusion: more high quality embryos and higher blastocyst rate can be obtained by using the embryo group culture model, and it is recommended that it can be used in the embryo culture of daily IVF treatment cycle.
【作者單位】： 溫州醫(yī)科大學(xué)附屬第二醫(yī)院生殖中心;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81370674) 溫州市科技局項(xiàng)目(Y20100205)
【分類號(hào)】：R714.8

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本文編號(hào)：1699177