本文選題：SIRT-1　切入點(diǎn)：western-blot　出處：《青島大學(xué)》2017年碩士論文

【摘要】：目的:探討正常子宮頸組織、宮頸不同級別上皮內(nèi)瘤變組織(低級別上皮內(nèi)瘤變組織、高級別上皮內(nèi)瘤變組織)及宮頸癌組織中SIRT-1蛋白的表達(dá)及其臨床意義。方法:收集2014-10-01至2016-01-30于青島大學(xué)醫(yī)學(xué)院附屬醫(yī)院婦科及青島市婦女兒童醫(yī)院婦科中心行手術(shù)治療或?qū)m頸活檢確診的患者140例,其中低級別上皮內(nèi)瘤變組織(LSIL)26例,高級別上皮內(nèi)瘤變組織(HSIL)35例及宮頸癌組織55例(包括47例鱗狀細(xì)胞癌,8例非鱗癌)。另選擇同期我院因子宮肌瘤行全子宮切除術(shù)后病理確診為正常宮頸的患者24例為對照。應(yīng)用免疫組織化學(xué)法及western-blot實(shí)驗(yàn)方法檢測SIRT-1蛋白的表達(dá)情況,并探討SIRT-1蛋白與宮頸癌患者臨床及各病理特征之間的相關(guān)性,統(tǒng)計(jì)學(xué)分析采用SPSS19.0。結(jié)果:1、免疫組化實(shí)驗(yàn)結(jié)果顯示SIRT-1蛋白主要在細(xì)胞核中表達(dá),棕黃色顆粒狀染色為陽性(見圖3-1);在正常子宮頸組織、低級別上皮內(nèi)瘤變組織、高級別上皮內(nèi)瘤變組織、癌性宮頸組織中的陽性表達(dá)率分別為0、15.38%、37.14%、40.00%,差異有統(tǒng)計(jì)學(xué)意義(X2=16.82,P0.05)。2.western-blot實(shí)驗(yàn)方法檢測中SIRT-1蛋白在正常宮頸組織、低級別宮頸上皮內(nèi)瘤變組(LSIL)、高級別宮頸上皮內(nèi)瘤變組(HSIL)及宮頸癌組中的表達(dá)情況與免疫組化實(shí)驗(yàn)的結(jié)果中SIRT-1蛋白表達(dá)基本上一致,四組間進(jìn)行比較存在顯著性差異,有統(tǒng)計(jì)學(xué)意義(F=13.86,P0.05)(見圖3-2)。3.宮頸癌組織中SIRT-1蛋白的表達(dá)與病理分化程度、有無淋巴結(jié)轉(zhuǎn)移及FIGO臨床分期有關(guān),分化程度越低陽性表達(dá)率越高,有顯著性差異(X2=6.87,P0.05);淋巴結(jié)轉(zhuǎn)移陽性組的陽性表達(dá)率高于淋巴結(jié)轉(zhuǎn)移陰性組,差異有顯著統(tǒng)計(jì)學(xué)意義(X2=7.73,P0.05);SIRT-1蛋白的陽性表達(dá)率隨臨床分期的增高而增加,有顯著統(tǒng)計(jì)學(xué)意義(X2=7.39,P0.05)。4.SIRT-1蛋白的表達(dá)與宮頸癌的組織學(xué)類型、患者的年齡無關(guān),差異無統(tǒng)計(jì)學(xué)意義(X2=1.03、0.038,P0.05)。結(jié)論:1.SIRT-1蛋白在正常宮頸組織、低級別上皮內(nèi)瘤變組織(LSIL)、高級別上皮內(nèi)瘤變組織(HSIL)、宮頸癌組織中的陽性表達(dá)率漸進(jìn)性增高,提示SIRT-1可能參與了正常宮頸組織-低級別上皮內(nèi)病變-高級別上皮內(nèi)病變-宮頸癌發(fā)生發(fā)展的漸進(jìn)過程。2.SIRT-1蛋白在組織病理學(xué)分化程度低、淋巴結(jié)轉(zhuǎn)移陽性、臨床分期晚的癌癥患者中高表達(dá),提示SIRT-1蛋白表達(dá)的異�？赡茉趯m頸癌的發(fā)生、進(jìn)展及轉(zhuǎn)移的過程中起到了重要作用。3.SIRT-1蛋白有望成為宮頸癌的早期診斷及預(yù)后判斷的生物學(xué)指標(biāo),為制定宮頸癌防治策略提供新的靶標(biāo)和科學(xué)證據(jù)。
[Abstract]:Objective: to study the normal cervical tissue, cervical intraepithelial neoplasia tissue of different grades (low grade intraepithelial neoplasia tissue), The expression and clinical significance of SIRT-1 protein in high grade intraepithelial neoplasia) and cervical carcinoma. Methods: from 2014-10-01 to 2016-01-30, the patients were operated at the gynecology center of the affiliated Hospital of Qingdao University Medical College and the Gynecological Center of Qingdao Women and Children's Hospital. 140 patients diagnosed by treatment or cervical biopsy, Among them, 26 cases of low grade intraepithelial neoplasia were found. 35 cases of high grade intraepithelial neoplasia and 55 cases of cervical carcinoma (including 47 cases of squamous cell carcinoma and 8 cases of non-squamous cell carcinoma) were selected. 24 cases of normal cervix were pathologically diagnosed after hysterectomy in our hospital during the same period. Immunohistochemical method and western-blot assay were used to detect the expression of SIRT-1 protein. To explore the correlation between SIRT-1 protein and clinical and pathological features of cervical cancer patients, SPSS 19.0. results showed that SIRT-1 protein was mainly expressed in the nucleus. Brown-yellow granular staining was positive (see Fig. 3-1; in normal cervical tissues, low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia, The positive expression rates of SIRT-1 protein in cervical cancer tissues were 0.15.38 and 37.14, respectively. The difference was statistically significant (P 0.05). 2. Western blot was used to detect SIRT-1 protein in normal cervix. The expression of SIRT-1 protein in low grade cervical intraepithelial neoplasia group, high grade cervical intraepithelial neoplasia group and cervical carcinoma group was basically consistent with the results of immunohistochemistry, and there was significant difference among the four groups. The expression of SIRT-1 protein was related to the degree of pathological differentiation, lymph node metastasis and clinical stage of FIGO. The lower the differentiation degree, the higher the positive expression rate. The positive expression rate of SIRT-1 protein in positive lymph node metastasis group was higher than that in negative lymph node metastasis group, and the positive expression rate of SIRT-1 protein increased with the increase of clinical stage. There was significant statistical significance between the expression of X2O7.39 P0.05N. 4. The expression of SIRT-1 protein was not related to the histological type of cervical cancer, but there was no significant difference in age of the patients. Conclusion: 1. SIRT-1 protein is expressed in normal cervical tissues. The positive expression rate of LSILA in low grade intraepithelial neoplasia and HSIL in high grade intraepithelial neoplasia was increased gradually in cervical carcinoma. These results suggest that SIRT-1 may be involved in the progressive process of normal cervix, low grade intraepithelial lesion, high grade intraepithelial lesion and cervical carcinogenesis. 2. SIRT-1 protein has low histopathological differentiation and positive lymph node metastasis. The overexpression of SIRT-1 protein in patients with late clinical stage of cancer suggests that the abnormal expression of SIRT-1 protein may occur in cervical cancer. SIRT-1 protein plays an important role in the progression and metastasis of cervical cancer. 3. SIRT-1 protein is expected to be a biological index for early diagnosis and prognosis of cervical cancer and provide new target and scientific evidence for the development of cervical cancer prevention and treatment strategy.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R737.33

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