本文選題：卵巢癌　切入點：結(jié)腸癌轉(zhuǎn)移相關(guān)基因　出處：《山東醫(yī)藥》2017年26期

【摘要】：目的觀察應(yīng)用基因沉默技術(shù)抑制人卵巢癌耐藥細胞結(jié)腸癌轉(zhuǎn)移相關(guān)基因1(MACC1)表達后,細胞增殖、凋亡和侵襲能力的變化及其分子機制。方法將人卵巢癌耐藥細胞株SKOV-3/DDP分成空質(zhì)粒組和轉(zhuǎn)染組,分別轉(zhuǎn)染空質(zhì)粒p-super-EGFP、重組質(zhì)粒p-super-EGFP-MACC1 shRNA,另取未經(jīng)處理的SKOV-3/DDP細胞作為空白對照組。采用RT-PCR方法檢測各組MACC1 mRNA表達,Western blotting法檢測MACC1蛋白表達,MTT法檢測細胞增殖能力,流式細胞術(shù)檢測細胞凋亡情況,體外黏附試驗檢測細胞體外黏附力,Transwell小室檢測細胞侵襲能力,Western blotting法測定C-met、ERK1/2、p-ERK1/2蛋白表達。結(jié)果與空白對照組和空質(zhì)粒組比較,轉(zhuǎn)染組MACC1 mRNA和蛋白表達減少,細胞48、72 h增殖能力減弱,細胞凋亡率增加,體外黏附和侵襲能力減弱,C-met、pERK1/2蛋白表達增加(P均0.05),而空白對照組和空質(zhì)粒組各指標(biāo)差異無統(tǒng)計學(xué)意義(P均0.05)。結(jié)論MACC1基因表達抑制后,SKOV-3/DDP的增殖、黏附和侵襲能力減弱,細胞凋亡增加,這可能與HGF/C-met和ERK通路受抑制有關(guān)。
[Abstract]:Objective to observe the effect of gene silencing on the proliferation of human ovarian cancer cells after inhibiting the expression of metastasis associated gene 1 (MACC1) in human ovarian cancer cells. Methods Human ovarian cancer cell line SKOV-3/DDP was divided into empty plasmid group and transfection group. The empty plasmids p-super-EGFP were transfected, the recombinant plasmid p-super-EGFP-MACC1 shRNAs were transfected, and the untreated SKOV-3/DDP cells were used as the blank control group. The expression of MACC1 mRNA in each group was detected by RT-PCR method and the expression of MACC1 protein was detected by Western blotting method. Flow cytometry was used to detect cell apoptosis, and in vitro adhesion assay was used to detect cell invasion ability. Western blotting assay was used to detect the expression of C-metERK1 / 2 p-ERK1 / 2 protein. The results were compared with those of blank control group and empty plasmid group. In the transfected group, the expression of MACC1 mRNA and protein decreased, the proliferation ability of the cells decreased and the apoptosis rate increased. The ability of adhesion and invasion was decreased in vitro. The expression of pERK1 / 2 protein increased significantly (P < 0.05), but there was no significant difference between blank control group and blank plasmid group (P < 0.05). Conclusion the proliferation, adhesion and invasion of SKOV-3 / DDP were decreased after the inhibition of MACC1 gene expression. The increase of apoptosis may be related to the inhibition of HGF/C-met and ERK pathways.
【作者單位】： 鄭州市婦幼保健院;鄭州大學(xué)第一附屬醫(yī)院;
【分類號】：R737.31

【相似文獻】

相關(guān)期刊論文 前5條

1 曹博淦;陳暉;;MACC1基因?qū)θ诵〖毎伟┘毎闟BC-5細胞生物學(xué)特性的影響[J];陜西醫(yī)學(xué)雜志;2013年10期

2 楊勇;胡孔旺;;MACC1在胃癌中的表達及其與血管形成的關(guān)系[J];安徽醫(yī)藥;2014年10期

3 尚超;張輝;宋永勝;;MACC1基因與腎癌轉(zhuǎn)移的相關(guān)性及其對細胞侵襲能力的影響[J];現(xiàn)代腫瘤醫(yī)學(xué);2011年04期

4 鄧佑興;史惠蓉;李霞;張瑞濤;;MACC1基因干擾對卵巢上皮性癌細胞增殖和順鉑耐藥的影響[J];中國癌癥雜志;2013年12期

5 ;[J];;年期

相關(guān)碩士學(xué)位論文 前5條

1 付彪;HIF-1α、MACC1、FOXO4與胃癌病理和預(yù)后的關(guān)系[D];安徽醫(yī)科大學(xué);2014年

2 呂煊;MACC1對前列腺癌體外侵襲轉(zhuǎn)移能力的影響[D];華中科技大學(xué);2011年

3 武雅君;MACC1基因在胃癌術(shù)后復(fù)發(fā)中的臨床意義及其可能機制探索[D];南方醫(yī)科大學(xué);2013年

4 李霞;MACC1基因在人卵巢癌順鉑化療耐藥株中的表達及對細胞耐藥性的影響[D];鄭州大學(xué);2014年

5 王剛;MACC1在胰腺癌中的表達及其對增殖、遷移及耐藥等功能影響的實驗研究[D];浙江大學(xué);2012年

，

本文編號：1669299