本文選題：卵巢子宮內膜異位癥　切入點：不孕癥　出處：《青島大學》2017年碩士論文

【摘要】：目的:觀察兩個標記基因含黃素單氧化酶3(FMO3,在輸卵管上皮組織高表達)、腦惡性腫瘤缺失基因(DMBT1,在子宮內膜組織高表達)分別在卵巢子宮內膜異位癥(EMs)不孕患者和已育患者異位病灶組織及正常卵巢組織中m RNA和蛋白表達水平,探討卵巢子宮內膜異位癥的異位病灶組織不同來源是否與其相關性不孕有關。方法:收集2014年4月~2015年8月期間在青島大學附屬醫(yī)院住院手術治療患者,90例卵巢子宮內膜異位癥患者根據是否伴有不孕分為卵巢EMs+不孕(40例)、卵巢EMs+已育(50例),兩組異位病灶組織分別作為觀察組和對照組。取因子宮肌瘤手術的35例患者正常卵巢組織作為正常組。采用Real-Time PCR法檢測FMO3、DMBT1m RNA表達水平,采用Western blotting法檢測FMO3、DMBT1蛋白表達水平。結果:1.觀察組中兩個標記基因FMO3、DMBT1在m RNA上表達水平均高于正常組,DMBT1上調為正常組的22.073-53.097(平均為33.953)倍,FMO3上調為正常組的2.058-6.096(平均為4.793)倍,且差異均有顯著的統(tǒng)計學意義(P均0.05);2.對照組中僅有DMBT1在m RNA表達水平高于正常組,上調水平為正常組的5.892-12.436(平均為9.619)倍,兩者間的差異有顯著的統(tǒng)計學意義(P0.05)。FMO3在m RNA表達水平無明顯增加,表達為正常組的1.501-2.131(平均為1.816)倍,兩者間無顯著的統(tǒng)計學意義(P0.05);3.觀察組與對照組相比,觀察組FMO3在m RNA表達水平明顯升高,上調為1.738-8.500(平均為3.847)倍,差異有顯著的統(tǒng)計學意義(P0.05)。DMBT1在m RNA的上調倍數為1.013-4.514(平均2.116),但其兩組間的差異無顯著的統(tǒng)計學意義(P0.05);4.與正常組DMBT1、FMO3的蛋白表達水平(分別為0.726-0.843(平均為0.780)、0.659-0.723(平均為0.699))相比,觀察組中DMBT1、FMO3的蛋白表達水平均明顯升高,分別為1.834-2.756(平均為1.600)、1.143-1.356(平均為1.259),且差異均具有顯著的統(tǒng)計學意義;5.與正常組相比,對照組中DMBT1的蛋白表達水平明顯升高,其表達水平為1.565-2.286(平均為1.400),差異有明顯的統(tǒng)計學意義(P0.05)。FMO3蛋白表達水平為0.826-0.941(平均為0.880),兩組中的表達水平無顯著差異(P0.05);6.實驗組與對照組相比,FMO3的蛋白表達水平明顯升高,且差異具有顯著的統(tǒng)計學意義(P0.05)。DMBT1的蛋白表達水平在兩者間無明顯區(qū)別,其差異不具有顯著的統(tǒng)計學意義(P0.05)。結論:卵巢EMs相關性不孕患者的異位病灶組織高表達FMO3、DMBT1,提示不孕患者的異位病灶組織來源可能為輸卵管上皮組織和子宮內膜組織;卵巢EMs合并已育患者異位病灶組織僅高表達DMBT1,FMO3的表達無明顯升高,提示已育患者異位病灶組織可能來源為子宮內膜組織;輸卵管組織來源的異位內膜組織可能與卵巢Ems患者不孕有關。
[Abstract]:Objective: to observe the expression of two marker genes, FMO3, in epithelial tissue of fallopian tube, and in ovarian endometriosis (EMs), respectively, and the deletion gene of DMBT1 in malignant tumor of brain (high expression in endometrial tissue); The expression level of m RNA and protein in ectopic focus tissue and normal ovarian tissue were detected. To investigate whether different sources of ectopic focus tissue in endometriosis of ovary are related to its associated infertility. Methods: from April 2014 to August 2015, 90 patients with ovarian cancer were treated by surgical treatment in Qingdao University affiliated Hospital. According to whether endometriosis is accompanied by infertility, 40 cases of ovarian EMs infertility and 50 cases of ovarian EMs have been divided into two groups: observation group and control group. 35 patients with uterine leiomyoma were treated as normal. Real-Time PCR assay was used to detect the expression of DMBT1m RNA in ovarian tissues. Western blotting method was used to detect the expression of FMO3 / DMBT1 protein. Results: 1. The expression level of two marker genes FMO3 / DMBT1 on m RNA in the observation group was higher than that in the normal group (22.073-53.097 (mean 33.953)) and the expression of FMO3 was 2.058-6.096 (mean 4.793) times higher than that in the normal group. The expression of DMBT1 in the control group was higher than that in the normal group, and the upregulation level was 5.892-12.436 (9.619) times higher than that in the normal group. There was no significant difference between the two groups in the expression level of m RNA, which was 1.501-2.131 times of normal group (average 1.816), and no significant statistical significance was found between the two groups. Compared with the control group, the expression of P0.05FMO3 in the observation group was higher than that in the control group, and there was no significant difference between the two groups. In the observation group, the expression of FMO3 in m RNA was significantly increased by 1.738-8.500 (mean 3.847) times. There was significant difference in the upregulation multiple of P0.05N. DMBT1 in m RNA (mean 2.116), but there was no significant difference between the two groups. The protein expression level of DMBT1 / FMO3 was 0.726-0.843 (mean 0.7800.659-0.723) in the normal group, but there was no significant difference between the two groups. The protein expression level of DMBT1 / FMO3 was significantly increased in the observation group (1.834-2.756 (mean 1.600-1.356), and the difference was significant statistically significant (P < 0.05). Compared with the control group, the expression of DMBT1 protein in the control group was significantly higher than that in the control group. The expression level of FMO3 was 1.565-2.286 (average 1.400), and the difference was significant (P 0.05) .FMO3 protein expression was 0.826-0.941 (mean 0.880). There was no significant difference between the two groups (P 0.05). Compared with the control group, the expression level of FMO3 in the experimental group was significantly higher than that in the control group, and the expression level of FMO3 in the experimental group was significantly higher than that in the control group, and the expression of FMO3 in the experimental group was significantly higher than that in the control group. There was no significant difference in the protein expression of P0.05U. DMBT1 between the two groups. Conclusion: the expression of FMO3 and DMBT1 in the ectopic lesions of patients with ovarian EMs associated infertility is high, suggesting that the ectopic lesions of infertile patients may originate from the tubal epithelial tissue and endometrial tissue. The expression of DMBT1FMO3 was not significantly increased in ovarian EMs complicated with ectopic lesions, suggesting that the ectopic focus tissues of fertile patients may originate from endometrial tissue. Ectopic endometrial tissue derived from oviduct tissue may be associated with infertility in patients with ovarian Ems.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R711.71;R711.6

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