本文選題：上皮性卵巢癌　切入點(diǎn)：miR-146a　出處：《中南大學(xué)》2014年碩士論文

【摘要】：目的： 檢測miR-146a在正常卵巢組織、良性上皮性卵巢腫瘤組織、上皮性卵巢癌組織中的表達(dá)水平并結(jié)合臨床病理參數(shù)資料進(jìn)行分析,初步探討miR-146a在上皮性卵巢癌中發(fā)生發(fā)展過程中的可能作用；進(jìn)一步檢測正常卵巢組織和上皮性卵巢癌組織中miR-146a靶基因IR-AK1蛋白分子表達(dá)情況,初步探討miR-146a在上皮性卵巢癌中可能的分子調(diào)控通路。 研究方法： 1.采用實時熒光定量PCR法檢測正常卵巢組織、良性上皮性卵巢腫瘤組織、上皮性卵巢癌組織中miR-146a的相對表達(dá)水平,并分析miR-146a表達(dá)和各臨床病理參數(shù)的關(guān)系。 2.運(yùn)用Western-Blotting檢測正常卵巢組織和上皮性卵巢癌組織中miR-146a靶基因IRAK1蛋白分子相對表達(dá)水平并分析上皮性卵巢癌中miR-146a與IRAK1表達(dá)相關(guān)性。 結(jié)果： 1.在正常的卵巢組織、良性上皮性卵巢腫瘤以及上皮性卵巢癌組織中,miR-146a的相對表達(dá)量逐漸升高,與正常卵巢組織相比,miR-146a表達(dá)在良性上皮性卵巢腫瘤及上皮性卵巢癌組織中明顯升高,差異有統(tǒng)計學(xué)意義(P0.05)；且上皮性卵巢癌組織中miR-146a的表達(dá)明顯高于良性上皮性卵巢腫瘤,差異有統(tǒng)計學(xué)意義(P0.05)。 2. miR-146a的相對表達(dá)量在低分化上皮性卵巢癌組中的明顯高于高-中分化上皮性卵巢癌組,差異具有統(tǒng)計學(xué)意義(P=0.006)；在不同年齡、FIGO分期、CA125水平組中表達(dá)均無統(tǒng)計學(xué)意義(P均0.05)。 3.與正常卵巢組織相比,IRAKI蛋白表達(dá)水平在過表達(dá)miR-146a的上皮性卵巢癌組織中明顯降低,差異具有統(tǒng)計學(xué)意義(P0.05),且兩者表達(dá)水平呈明顯的負(fù)相關(guān)(r=-0.78,P0.05)。 結(jié)論： 1.上皮性卵巢癌組織中miR-146a表達(dá)水平相比于正常卵巢組織和良性卵巢上皮性腫瘤組織明顯上調(diào),可能參與了上皮性卵巢癌的發(fā)生及調(diào)控。 2.miR-146a表達(dá)水平在上皮性卵巢癌低分化組中明顯高于高-中分化組,miR-146a可能與上皮性卵巢癌腫瘤細(xì)胞惡性程度有關(guān)。 3.過表達(dá)miR-146a的上皮性卵巢組織中IRAK1蛋白分子顯著下調(diào),兩者呈顯著負(fù)相關(guān),在蛋白分子水平上支IRAK1是miR-146a的靶基因,miR-146a可能通過靶向抑制IRAK1mRNA的翻譯,調(diào)控上皮性卵巢癌細(xì)胞的生物學(xué)功能。
[Abstract]:Objective:. The expression of miR-146a in normal ovarian tissue, benign epithelial ovarian tumor tissue and epithelial ovarian carcinoma tissue was detected and analyzed with clinicopathological data. To explore the possible role of miR-146a in the occurrence and development of epithelial ovarian cancer, and to detect the expression of miR-146a target gene IR-AK1 protein in normal ovarian tissues and epithelial ovarian cancer tissues. To explore the possible molecular regulatory pathway of miR-146a in epithelial ovarian cancer. Research methods:. 1. The relative expression of miR-146a in normal ovarian tissues, benign epithelial ovarian tumor tissues and epithelial ovarian cancer tissues was detected by real-time fluorescence quantitative PCR. The relationship between miR-146a expression and clinicopathological parameters was analyzed. 2. The relative expression of miR-146a target gene IRAK1 protein in normal ovarian tissues and epithelial ovarian carcinoma tissues was detected by Western-Blotting and the correlation between miR-146a and IRAK1 expression in epithelial ovarian carcinoma was analyzed. Results:. 1. In normal ovarian tissues, the relative expression of miR-146a in benign epithelial ovarian tumors and epithelial ovarian cancer tissues increased gradually. The expression of miR-146a in benign epithelial ovarian tumor and epithelial ovarian carcinoma was significantly higher than that in normal ovarian tissue (P 0.05), and the expression of miR-146a in epithelial ovarian carcinoma was significantly higher than that in benign epithelial ovarian tumor. The difference was statistically significant (P 0.05). 2. The relative expression of miR-146a in poorly differentiated epithelial ovarian carcinoma group was significantly higher than that in well-moderately differentiated epithelial ovarian carcinoma group, and the difference was statistically significant (P < 0.05). 3. The expression of IRAKI protein in epithelial ovarian carcinoma with overexpression of miR-146a was significantly lower than that in normal ovarian tissues, and the difference was statistically significant (P 0.05), and there was a significant negative correlation between the expression level of IRAKI and the expression of IRAKI protein in epithelial ovarian carcinoma tissues with overexpression of miR-146a. Conclusion:. 1. The expression of miR-146a in epithelial ovarian carcinoma was significantly higher than that in normal ovarian tissues and benign ovarian epithelial tumor tissues, which may be involved in the genesis and regulation of epithelial ovarian cancer. The expression of 2.miR-146a in the poorly differentiated epithelial ovarian carcinoma group was significantly higher than that in the high and medium differentiation group. The expression of miR-146a might be related to the malignancy of epithelial ovarian cancer cells. 3. IRAK1 protein molecules were significantly down-regulated in epithelial ovarian tissues with overexpression of miR-146a, and there was a significant negative correlation between them. At the protein molecular level, IRAK1 was the target gene of miR-146a, and miR-146a might inhibit the translation of IRAK1mRNA by targeting. The biological function of epithelial ovarian cancer cells is regulated.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R737.31

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