本文選題：吲哚美辛　切入點(diǎn)：宮頸癌Hela細(xì)胞　出處：《中國藥房》2015年31期 　論文類型：期刊論文

【摘要】：目的:研究吲哚美辛對宮頸癌Hela細(xì)胞增殖、凋亡的影響。方法:以體外培養(yǎng)的Hela細(xì)胞為試驗(yàn)對象,分別用0(空白對照)、200、400、600、800、1 000μmol/L吲哚美辛作用24、48、72 h后,采用MTT法檢測吲哚美辛對Hela細(xì)胞的增殖抑制率;用0(空白對照)、400、600、800μmol/L吲哚美辛作用24 h后,采用倒置顯微鏡觀察細(xì)胞形態(tài)的改變,并用流式細(xì)胞儀檢測細(xì)胞周期分布和細(xì)胞凋亡情況。結(jié)果:600、800、1 000μmol/L吲哚美辛可抑制Hela細(xì)胞的增殖,增殖抑制率與藥物濃度和作用時(shí)間呈正相關(guān)。與空白對照比較,吲哚美辛可使Hela細(xì)胞形態(tài)由多角形逐漸向圓形轉(zhuǎn)變且細(xì)胞呈現(xiàn)凋亡和壞死狀態(tài);其中細(xì)胞G0/G1期比例增加、S期比例減少,細(xì)胞凋亡率增加。結(jié)論:吲哚美辛可抑制Hela細(xì)胞增殖,誘導(dǎo)其凋亡,主要阻滯細(xì)胞于G0/G1期。
[Abstract]:Objective: to study the effect of indomethacin on the proliferation and apoptosis of cervical cancer Hela cells. Methods: Hela cells cultured in vitro were treated with indomethacin for 244872 hours. The inhibitory rate of indomethacin on the proliferation of Hela cells was detected by MTT assay, and the morphological changes of Hela cells were observed by inverted microscope after 24 hours of exposure to indomethacin at 600 渭 mol/L. Cell cycle distribution and apoptosis were detected by flow cytometry. Results the proliferation of Hela cells was inhibited by 1 000 渭 mol/L of 1 000 渭 mol/L. The inhibitory rate of proliferation was positively correlated with the concentration and time of action. Indomethacin could gradually change the shape of Hela cells from polygonal to circular and present apoptosis and necrosis, in which the proportion of G _ 0 / G _ 1 phase increased in S phase and the apoptosis rate increased. Conclusion: indomethacin can inhibit the proliferation of Hela cells. Apoptosis was induced, mainly in G _ 0 / G _ 1 phase.
【作者單位】： 浙江省人民醫(yī)院藥學(xué)部;浙江中醫(yī)藥大學(xué)藥學(xué)院;
【分類號】：R737.33

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相關(guān)期刊論文 前7條

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本文編號：1633288