本文選題：維生素E　切入點(diǎn)：妊娠糖尿病　出處：《桂林醫(yī)學(xué)院》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：探討維生素E（Ve）及葉酸對(duì)高血糖誘導(dǎo)的乳鼠心肌細(xì)胞凋亡蛋白capase-3表達(dá)的影響。方法：將50只清潔級(jí)成年SD孕鼠隨機(jī)分為5組，分別為正常對(duì)照組(A組)、妊娠糖尿病組(B組)、葉酸組(C組)、維生素E組(D組)和Ve+葉酸組(E組)。除對(duì)照組外，其余大鼠于妊娠第3天腹腔注射鏈脲佐菌素(STZ）35mg/kg制備妊娠糖尿病動(dòng)物模型，對(duì)照組給予等量0.1mmol/L（PH4.5）檸檬酸鈉—檸檬酸緩沖液。孕第5天，VE組、VE+葉酸組兩組給予50mg/kg維生素E灌胃，葉酸組、VE+葉酸組予0.4mg/kg葉酸灌胃，A、B組予等量的生理鹽水灌胃，持續(xù)7天。取自然分娩的乳鼠心肌組織于光鏡下觀察心肌結(jié)構(gòu)的改變，應(yīng)用免疫組織化學(xué)法檢測各組乳鼠心肌細(xì)胞caspase-3抗原的表達(dá)。蛋白免疫印跡(western blot)檢測各組乳鼠心肌細(xì)胞凋caspase-3的表達(dá)。結(jié)果：1.造模前各組血糖值比較差異無統(tǒng)計(jì)學(xué)意義（p0.05）；造模后，糖尿病、葉酸組、VE組、VE+葉酸組血糖值明顯高于A組，差異有統(tǒng)計(jì)學(xué)意義（p0.01）；糖尿病、葉酸組、VE組、VE+葉酸組四組間比較差異無統(tǒng)計(jì)學(xué)意義（p0.05）。2.光鏡下觀察糖尿病、葉酸組、VE組、VE+葉酸組乳鼠心肌組織呈現(xiàn)不同程度損傷，表現(xiàn)為細(xì)胞壞死、水腫、輪廓不清，心肌纖維排列紊亂、炎性細(xì)胞浸潤。3.5組間乳鼠心肌細(xì)胞caspase-3平均吸光度值兩兩比較，差異有統(tǒng)計(jì)學(xué)意義（p0.01）。4.5組胎鼠心肌細(xì)胞caspase-3蛋白表達(dá)灰度值兩兩比較，差異有統(tǒng)計(jì)學(xué)意義（p0.01）結(jié)論：孕鼠妊娠期高血糖可導(dǎo)致胎鼠心肌細(xì)胞凋亡增多，補(bǔ)充VE和葉酸對(duì)妊娠高血糖所致的胎兒心肌細(xì)胞凋亡有保護(hù)作用，葉酸抑制胎鼠心肌凋亡的作用強(qiáng)于VE，，聯(lián)合補(bǔ)充VE和葉酸較單純補(bǔ)充VE或葉酸效果更佳，但不能使其恢復(fù)正常水平。
[Abstract]:Objective: to investigate the effects of Vitamin E (Veg) and folic acid on the expression of apoptotic protein (capase-3) in neonatal rat cardiomyocytes induced by hyperglycemia. Methods: fifty adult SD pregnant rats of clean grade were randomly divided into 5 groups. They were normal control group A, gestational diabetes mellitus group B, folic acid group C group, vitamin E group D) and ve folic acid group. The other rats were intraperitoneally injected with STZ 35 mg / kg on the third day of pregnancy to make gestational diabetic animal model, the control group was given the same amount of 0.1 mmol / L PH4.5) citrate sodium citric acid buffer, and on the 5th day of pregnancy, the VE folic acid group and the VE folic acid group were given 50 mg / kg vitamin E intragastrically. The rats in folic acid group were given 0.4 mg / kg folic acid intragastric perfusion with normal saline for 7 days. The myocardial structure of naturally delivered neonatal rats was observed under light microscope. Immunohistochemical method was used to detect the expression of caspase-3 antigen in neonatal rat cardiomyocytes. Western blotting was used to detect the expression of caspase-3 in neonatal rat cardiomyocytes. Results: 1. There was no significant difference in blood glucose levels before and after modeling. In diabetes mellitus, the blood glucose level in VE group was significantly higher than that in A group, the difference was statistically significant (p 0.01), and there was no significant difference among the four groups in diabetes mellitus (DM) and folic acid group (VE). In folic acid group, VE group showed different degree of injury in myocardial tissue, including cell necrosis, edema, unclear outline, disordered arrangement of myocardial fibers, inflammatory cell infiltration. The average caspase-3 absorbance of myocardial cells of neonatal rats in 3.5 groups was compared in pairs. There was a significant difference in the expression of caspase-3 protein in fetal cardiomyocytes between the two groups (P < 0.01). Conclusion: hyperglycemia during pregnancy can lead to the increase of cardiomyocyte apoptosis in fetal rats. Vitamin E and folic acid supplementation had protective effect on fetal cardiomyocyte apoptosis induced by pregnancy hyperglycemia. Folic acid had stronger effect on inhibiting fetal myocardial apoptosis than VE.The combination of VE and folic acid was better than vitamin E or folic acid alone. But it cannot be brought back to normal.
【學(xué)位授予單位】：桂林醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R714.256

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