本文選題：妊娠高血壓綜合征　切入點(diǎn)：母-胎免疫耐受　出處：《中國(guó)免疫學(xué)雜志》2017年09期 　論文類型：期刊論文

【摘要】：目的:分析妊娠高血壓綜合征(PIH)患者外周血中免疫細(xì)胞因子水平變化及滋養(yǎng)層細(xì)胞中免疫相關(guān)轉(zhuǎn)錄因子的基因、蛋白表達(dá)情況,探討母-胎免疫調(diào)節(jié)機(jī)制在妊娠高血壓綜合征病理過(guò)程中的作用。方法:納入PIH患者50例作為觀察組,選擇同期分娩的正常妊娠婦女40例作為對(duì)照組。ELISA法檢測(cè)兩組孕婦外周血中IFN-γ、IL-4、IL-17及TGF-β水平;實(shí)時(shí)熒光定量PCR(RT-q PCR)法和蛋白質(zhì)印跡法(Western blot)分別測(cè)定兩組孕婦胎盤滋養(yǎng)層細(xì)胞中轉(zhuǎn)錄因子T-bet、GATA-3、RORC、Foxp3的mRNA及蛋白表達(dá)情況。結(jié)果:(1)觀察組外周血中IFN-γ和IL-17水平明顯高于對(duì)照組,TGF-β水平明顯低于對(duì)照組(P0.05);兩組間IL-4水平無(wú)明顯差異(P0.05);觀察組IFN-γ/IL-4、IL-17/TGF-β比值均明顯大于對(duì)照組(P0.05)。(2)與對(duì)照組比,觀察組T-bet、RORC的mRNA及蛋白表達(dá)水平均顯著升高(P0.05),而GATA-3、Foxp3的mRNA及蛋白表達(dá)水平均顯著降低(P0.05),T-bet/GATA-3及RORC/Foxp3比值均顯著增大(P0.05)。結(jié)論:PIH具有明顯的Th1/Th2及Th17/Treg比例失衡現(xiàn)象,其病理機(jī)制可能與Th細(xì)胞介導(dǎo)的母-胎免疫耐受機(jī)制功能紊亂有關(guān)。
[Abstract]:Objective: to analyze the pregnancy induced hypertension syndrome (PIH) gene immune changes in immune cytokines in the peripheral blood and trophoblast cells in the transcription factors related to patients, protein expression and regulation mechanism of maternal fetal immune syndrome in the pathological process for pregnancy induced hypertension. Methods: Sixty PIH patients 50 cases as the observation group during the same period, select 40 cases of normal pregnant women as control group.ELISA detection method IFN- two groups of pregnant women in the peripheral blood of IL-4, IL-17 and TGF- gamma, beta level; real time fluorescence quantitative PCR (RT-q PCR) and Western blotting (Western blot) transcription factor T-bet, two groups of pregnant women were measured in placental trophoblast cells GATA-3, RORC, mRNA and protein expression of Foxp3. Results: (1) observation group peripheral blood IFN- and IL-17 levels were significantly higher than the control group, TGF- levels were significantly lower than the control group (P0.05); IL-4 in the two groups no significant difference ISO (P0.05); the observation group IFN- beta gamma /IL-4, IL-17/TGF- ratio was significantly higher than the control group (P0.05). (2) compared with control group, the observation group T-bet, mRNA and protein expression levels of RORC were significantly increased (P0.05), GATA-3, mRNA and protein expression levels of Foxp3 were significantly lower (P0.05), T-bet/GATA-3 and RORC/Foxp3 ratio were significantly increased (P0.05). Conclusion: PIH has Th1/Th2 and Th17/Treg imbalance phenomenon obviously, the pathological mechanism may be mediated by Th cells in maternal fetal immune tolerance mechanism dysfunction.

【作者單位】： 皖南醫(yī)學(xué)院弋磯山醫(yī)院婦產(chǎn)科;皖南醫(yī)學(xué)院弋磯山醫(yī)院藥劑科;
【分類號(hào)】：R714.246

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1 王越,葉路,付正英,潘菊芬;妊娠高血壓綜合征發(fā)病中免疫調(diào)節(jié)機(jī)制的研究[J];免疫學(xué)雜志;2004年02期

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本文編號(hào)：1609659