本文選題：白念珠菌　切入點(diǎn)：隨機(jī)擴(kuò)增多態(tài)性DNA　出處：《中國(guó)感染與化療雜志》2015年04期 　論文類型：期刊論文

【摘要】：目的了解上海地區(qū)白念珠菌主要基因型,為監(jiān)測(cè)和診治臨床白念珠菌感染提供實(shí)驗(yàn)依據(jù)。方法收集2013年11月—2014年1月復(fù)旦大學(xué)附屬婦產(chǎn)科醫(yī)院、國(guó)際和平婦幼保健院和上海市第一婦嬰保健院3所醫(yī)院陰道炎患者陰道分泌物分離的白念珠菌共115株,隨機(jī)擴(kuò)增多態(tài)性DNA檢測(cè)其基因型,ATB FUNGUS 3試劑盒檢測(cè)5種抗真菌藥物最低抑菌濃度。結(jié)果隨機(jī)擴(kuò)增多態(tài)性DNA結(jié)果顯示,115株菌分為A、B、C3型,其中A型89株(占77.4%)又分5個(gè)亞型,B型22株(占19.1%)分為2個(gè)亞型。C型4株(占3.5%)。115株白念珠菌對(duì)5-氟胞嘧啶敏感率為96.5%;對(duì)兩性霉素B敏感率為100%;對(duì)氟康唑敏感率為85.2%;對(duì)伊曲康唑敏感率為55.7%;對(duì)伏立康唑敏感率為84.3%。結(jié)論上海地區(qū)3所醫(yī)院分離的白念珠菌基因型沒(méi)有明顯區(qū)別,同源性分型主要以A型為主。藥敏試驗(yàn)顯示,該地區(qū)白念珠菌對(duì)5-氟胞嘧啶、兩性霉素B、氟康唑、伏立康唑敏感率較高。
[Abstract]:Objective to investigate the major genotypes of Candida albicans in Shanghai and to provide experimental evidence for the monitoring and diagnosis of clinical Candida albicans infection. Methods from November 2013 to January 2014, the hospital of gynecology and obstetrics affiliated to Fudan University was collected. A total of 115 strains of Candida albicans isolated from vaginal secretions of vaginitis patients in the three hospitals of International Peace Maternal and Child Health Hospital and Shanghai first Maternal and Child Health Hospital were isolated. Random amplified polymorphic DNA (RAPD) was used to detect the minimum inhibitory concentration of five antifungal drugs. Of these, 89 strains of type A (77.4%) were further divided into 5 subtypes, 22 strains of subtype B (19.1%), and 4 strains of subtype C. C (3.55%). 115 strains of Candida albicans were found to be sensitive to 5-fluorocytosine 96.5%, amphotericin B sensitivity to 100 batches and fluconazole sensitivity to fluconazole. The sensitivity rate to itraconazole was 55.7%, the sensitivity rate to voriconazole was 84.30.Conclusion there was no significant difference in the genotypes of Candida albicans isolated from 3 hospitals in Shanghai. The susceptibility of Candida albicans to 5-fluorocytosine, amphotericin B, fluconazole and fulconazole was high.
【作者單位】： 復(fù)旦大學(xué)附屬婦產(chǎn)科醫(yī)院檢驗(yàn)科;國(guó)際和平婦幼保健院;上海市第一婦嬰保健院;
【基金】：上海申康醫(yī)院發(fā)展中心市級(jí)醫(yī)院臨床輔助科室能力建設(shè)項(xiàng)目(SHDC22014016)
【分類號(hào)】：R711.31;R446.19

【參考文獻(xiàn)】

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本文編號(hào)：1568996