發(fā)布時間：2018-02-27 23:23

  本文關(guān)鍵詞： 未見原核卵裂胚胎 囊胚 拷貝數(shù)變異　出處：《中國現(xiàn)代醫(yī)學(xué)雜志》2017年09期 　論文類型：期刊論文

【摘要】：目的對常規(guī)體外受精(IVF)中未見原核卵裂胚胎囊胚滋養(yǎng)層細(xì)胞10 MB拷貝數(shù)變異進(jìn)行分析。方法對IVF受精的未見原核卵裂胚胎囊胚培養(yǎng),利用全基因組測序的方法對D5評級3BC囊胚的滋養(yǎng)層細(xì)胞進(jìn)行10MB的拷貝數(shù)變異檢測,判斷未見原核卵裂胚胎囊胚非整倍體變異程度。結(jié)果共選擇未見原核卵裂胚胎69例,形成囊胚51例(73.91%);囊胚評級3BC者共33例,對其進(jìn)行滋養(yǎng)層細(xì)胞活檢并進(jìn)行拷貝數(shù)變異檢測,檢測成功23例(70%),其中正常核型(46,XN)17例(73.9%),異常6例(26.1%)。結(jié)論未見原核卵裂胚胎經(jīng)囊胚培養(yǎng)可以獲得較高比例染色體正常胚胎,結(jié)合胚胎植入前遺傳學(xué)診斷及產(chǎn)前診斷技術(shù),未見原核卵裂囊胚具有利用價值。
[Abstract]:Objective to analyze the variation of 10 MB copy number in trophoblast cells of prokaryotic cleavage embryo in vitro fertilization (IVF). Methods to culture the blastocyst without prokaryotic cleavage in IVF fertilization. A total of 10 MB copy number variation was detected in trophoblast cells of D5 rated 3BC blastocysts by whole genome sequencing, and the degree of aneuploidy of blastocysts without prokaryotic cleavage was determined. Results 69 non-prokaryotic blastocysts were selected. There were 51 cases of blastocyst and 33 cases of blastocyst with 3BC grade. The trophoblastic cell biopsy was performed and copy number variation was detected. 23 cases were detected successfully, of which 17 cases were normal karyotype 46 X Ns, and 6 cases were abnormal. Conclusion A high proportion of normal chromosomal embryos can be obtained by blastocyst culture in prokaryotic cleavage embryos, combined with genetic diagnosis and prenatal diagnosis techniques before embryo implantation. No prokaryotic blastocysts were found to be useful.
【作者單位】： 內(nèi)蒙古醫(yī)科大學(xué)附屬醫(yī)院生殖中心;
【基金】：內(nèi)蒙古自然科學(xué)基金(No:2015BS0802) 內(nèi)蒙古教育廳基金(No:NJZY16118) 內(nèi)蒙古醫(yī)科大學(xué)科技百萬工程(No:YKD2014KJBW007、YKD2015KJBW023)
【分類號】：R714.8

【相似文獻(xiàn)】

相關(guān)期刊論文 前3條

1 劉曉妍;郝翠芳;張守信;沈肖方;單英華;黃鑫;;移植未受精晚期卵裂胚胎妊娠2例[J];中華婦幼臨床醫(yī)學(xué)雜志(電子版);2012年01期

2 張守信;劉曉妍;孫成銘;劉杰;郝翠芳;;未見原核晚期卵裂胚胎染色體的微陣列-比較基因組雜交分析[J];中華婦幼臨床醫(yī)學(xué)雜志(電子版);2013年06期

3 ;[J];;年期

相關(guān)重要報紙文章 前1條

1 孫美燕 胡玲;植入前遺傳學(xué)診斷妊娠成功[N];健康報;2002年

相關(guān)碩士學(xué)位論文 前1條

1 連偉玲;移植人類早期卵裂胚胎對體外受精—胚胎移植妊娠結(jié)局的影響[D];鄭州大學(xué);2012年

，

本文編號：1544766