發(fā)布時間：2018-02-25 05:26

  本文關(guān)鍵詞： 子宮腫瘤 子宮內(nèi)膜癌 蛋白激酶D 表達(dá) 病理診斷　出處：《臨床與實(shí)驗(yàn)病理學(xué)雜志》2017年01期 　論文類型：期刊論文

【摘要】：目的探討蛋白激酶D1(protein kinase D1,PKD1)在子宮內(nèi)膜癌組織和正常子宮內(nèi)膜組織中的表達(dá),并分析其與子宮內(nèi)膜癌臨床病理特征的關(guān)系。方法應(yīng)用免疫組化SP法及qRT-PCR法檢測92例子宮內(nèi)膜癌組織和48例正常子宮內(nèi)膜組織中PKD1 mRNA及其蛋白的表達(dá),分析PKD1在子宮內(nèi)膜癌組織中的表達(dá)及其與分化程度、臨床分期的關(guān)系。應(yīng)用Western blot法檢測PKD1蛋白在正常子宮內(nèi)膜細(xì)胞株及不同分化程度的子宮內(nèi)膜癌細(xì)胞株中的表達(dá)水平。結(jié)果子宮內(nèi)膜癌組織中的PKD1 mRNA及蛋白表達(dá)水平均顯著高于正常子宮內(nèi)膜組織(P0.01);PKD1 mRNA及蛋白表達(dá)與子宮內(nèi)膜癌的組織分化程度均有相關(guān)性,且組織分化程度越低,臨床病理分期越高,PKD1的表達(dá)越豐富。PKD1蛋白在子宮內(nèi)膜癌細(xì)胞株中的表達(dá)水平也遠(yuǎn)高于正常子宮內(nèi)膜細(xì)胞中的表達(dá)(P0.01),且癌細(xì)胞株的分化水平越低,PKD1蛋白表達(dá)的水平越高。結(jié)論P(yáng)KD1在子宮內(nèi)膜癌患者癌灶中呈高表達(dá),PKD1表達(dá)水平的高、低可以做為預(yù)測子宮內(nèi)膜癌惡性程度的一項(xiàng)重要參考依據(jù)。
[Abstract]:Objective to investigate the expression of protein kinase D 1 protein kinase D 1 protein in endometrial carcinoma and normal endometrium. Methods the expression of PKD1 mRNA and its protein in 92 cases of endometrial carcinoma and 48 cases of normal endometrium were detected by immunohistochemical SP method and qRT-PCR method. To analyze the expression and differentiation of PKD1 in endometrial carcinoma. Western blot method was used to detect the expression of PKD1 protein in normal endometrial cell line and endometrial cancer cell line with different differentiation degree. Results PKD1 mRNA and protein expression water in endometrial carcinoma tissue. The expression of PKD1 mRNA and protein in endometrial tissues was significantly higher than that in normal endometrial tissues, and there was a correlation between the expression of PKD1 mRNA and the degree of differentiation of endometrial carcinoma. And the lower the degree of tissue differentiation, The higher the clinicopathologic stage, the richer the expression of PKD1 protein. The expression level of PKD1 protein in endometrial cancer cell line is much higher than that in normal endometrial cell line, and the differentiation level of cancer cell line is lower. Conclusion the higher the expression of PKD1 in endometrial carcinoma, the higher the expression of PKD1. The low level can be used as an important reference for predicting the malignancy of endometrial carcinoma.
【作者單位】： 河南省南陽市第一人民醫(yī)院生殖婦科;
【基金】：國家自然科學(xué)基金(81473438) 河南省科技攻關(guān)課題(122102330049)
【分類號】：R737.33
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本文編號：1533189