本文關(guān)鍵詞： 人乳頭狀瘤病毒L1殼蛋白 白介素-2 宮頸上皮內(nèi)瘤變 鱗狀細(xì)胞癌 免疫組化　出處：《山西醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的檢測(cè)HPVL1蛋白和IL-2在宮頸病變中的表達(dá),探討二者與宮頸病變的的關(guān)系和臨床意義,為癌前病變及宮頸癌的診斷和治療提供實(shí)驗(yàn)依據(jù)。 方法選取2011年4月至2011年9月在山西省腫瘤醫(yī)院就診的宮頸液基細(xì)胞學(xué)檢查診斷≥未明確診斷意義的不典型鱗狀細(xì)胞(ASCUS),且檢測(cè)HPVDNA均為陽性,同時(shí)有組織病理學(xué)診斷(作為金標(biāo)準(zhǔn))患者的宮頸組織石蠟包埋標(biāo)本,采用免疫組織化學(xué)法結(jié)合應(yīng)用計(jì)算機(jī)圖像分析方法對(duì)檢測(cè)結(jié)果進(jìn)行圖像采集,并對(duì)實(shí)驗(yàn)數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析,觀察HPVL1蛋白和IL-2在宮頸上皮內(nèi)瘤病變及宮頸癌中的表達(dá)情況,從而研究HPVL1蛋白和IL-2與宮頸上皮內(nèi)瘤病變及宮頸癌之間的關(guān)系。結(jié)果免疫組織化學(xué)方法顯示：HPVL1蛋白的表達(dá)定位于細(xì)胞核,主要表達(dá)于宮頸組織的上皮層。159例標(biāo)本中,HPVL1陽性表達(dá)為47.80%(76例)。慢性宮頸炎、CINI、 CINⅡ、CINIII及SCC的陽性表達(dá)分別為62.50%(30/48)、83.33%(35/42)、37.03%(10/27)、3.3%(1/30)、0%(0/12)。除了CINIII與SCC比較,差異無統(tǒng)計(jì)學(xué)意義(P0.05)外,其他不同病變間的比較,差異有統(tǒng)計(jì)學(xué)意義(P均0.05)。IL-2表達(dá)定位于細(xì)胞膜和/或細(xì)胞質(zhì),主要表達(dá)于宮頸組織的上皮層。IL-2陽性表達(dá)為64.78%(103例)。慢性宮頸炎、CINⅠ、CINⅡ、CINIII及SCC的陽性表達(dá)分別為83.33%(40/48)、89.30%(37/42)、62.97%(17/27)、3.3%(9/30)及0%(0/12),差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論本實(shí)驗(yàn)研究結(jié)果顯示,在宮頸上皮內(nèi)瘤變組織中,HPVL1蛋白與IL-2的表達(dá)具有相關(guān)性(rs=0.165, p0.05)。HPVL1-/IL-2-的表達(dá)44例,其表達(dá)隨著宮頸病變的程度的增加而呈上升趨勢(shì),HPVL1+/IL-2+的表達(dá)64例,其表達(dá)隨著宮頸病變的程度的增加而呈下降趨勢(shì)。
[Abstract]:Objective to detect the expression of HPVL1 protein and IL-2 in cervical lesions and to explore the relationship between them and cervical lesions and to provide experimental evidence for the diagnosis and treatment of precancerous lesions and cervical cancer. Methods from April 2011 to September 2011, cervical liquid-based cytology was performed in Shanxi Provincial Cancer Hospital. Atypical squamous cells with no definite diagnostic significance were selected, and HPVDNA was positive. At the same time, paraffin embedded specimens of cervical tissues of patients with histopathological diagnosis (as gold standard) were collected by immunohistochemical method and computer image analysis. The expression of HPVL1 protein and IL-2 in cervical intraepithelial neoplasia and cervical carcinoma were analyzed statistically. The relationship between HPVL1 protein and IL-2 and cervical intraepithelial neoplasia and cervical carcinoma was studied. Results the expression of HPVL1 L1 protein was localized in the nucleus by immunohistochemical method. The positive expression of HPVL1 was 47.80 in 76 cases of cervical epithelium. The positive expression of CINI, CIN 鈪，

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