本文關(guān)鍵詞： F基因 宮頸癌 表達(dá)分析　出處：《南方醫(yī)科大學(xué)學(xué)報(bào)》2017年06期 　論文類型：期刊論文

【摘要】：目的檢測(cè)F10基因及其編碼蛋白在宮頸癌組織中的表達(dá)情況,探討F10基因與宮頸癌病理發(fā)生發(fā)展的關(guān)系。方法收集宮頸癌臨床組織標(biāo)本共30例,運(yùn)用RT-PCR的方法檢測(cè)30對(duì)宮頸癌組織及相應(yīng)的癌旁組織中的F10基因的表達(dá),運(yùn)用免疫組織化學(xué)方法檢測(cè)并比較30對(duì)宮頸癌組織與癌旁組織及正常組織中F10基因蛋白的表達(dá)情況。結(jié)果 RT-PCR的檢測(cè)結(jié)果顯示,宮頸癌組織中F10基因的表達(dá)明顯高于相應(yīng)癌旁組織,差異有統(tǒng)計(jì)學(xué)意義(P0.05),免疫組織化學(xué)檢測(cè)F10蛋白的結(jié)果與RT-PCR結(jié)果基本一致。對(duì)不同分化程度的病例進(jìn)行分析,結(jié)果顯示低分化組宮頸癌組織中F10的表達(dá)顯著高于中低分化或中分化病例。術(shù)前化療的宮頸癌組織中F10的表達(dá)量降低,顯著低于未行術(shù)前化療的病例(P0.01)。結(jié)論宮頸癌組織中F10基因的表達(dá)量與其分化程度呈負(fù)相關(guān),分化程度越低的宮頸癌組織中F10表達(dá)越強(qiáng),可能與癌癥的產(chǎn)生與發(fā)展有關(guān)。另外,化療顯著下調(diào)宮頸癌組織中F10的表達(dá)。
[Abstract]:Objective to detect the expression of F10 gene and its coding protein in cervical carcinoma and to explore the relationship between F10 gene and pathological development of cervical carcinoma. Methods 30 clinical specimens of cervical cancer were collected. The expression of F10 gene in 30 pairs of cervical cancer tissues and corresponding adjacent tissues was detected by RT-PCR. Immunohistochemical method was used to detect and compare the expression of F10 gene protein in 30 pairs of cervical cancer tissues, adjacent tissues and normal tissues. The expression of F10 gene in cervical cancer tissues was significantly higher than that in corresponding adjacent tissues, and the difference was statistically significant (P 0.05). The results of immunohistochemical detection of F10 protein were consistent with those of RT-PCR. The results showed that the expression of F10 in poorly differentiated cervical cancer tissues was significantly higher than that in moderately or moderately differentiated cases. Conclusion there is a negative correlation between the expression of F10 gene and the degree of differentiation in cervical carcinoma. The stronger the expression of F10 in the cervical carcinoma with lower differentiation, may be related to the occurrence and development of cancer, and the expression of F10 gene in cervical cancer is significantly lower than that in patients without preoperative chemotherapy. In addition, the expression of F10 gene in cervical cancer is negatively correlated with the degree of differentiation. Chemotherapy significantly down-regulated the expression of F10 in cervical carcinoma.
【作者單位】： 南方醫(yī)科大學(xué)南方醫(yī)院婦產(chǎn)科;
【基金】：國(guó)家自然科學(xué)基金(81170595)~~
【分類號(hào)】：R737.33

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊金娣;龐戰(zhàn)軍;;F10基因沉默及過(guò)表達(dá)對(duì)絨癌細(xì)胞系JAR細(xì)胞周期的影響[J];南方醫(yī)科大學(xué)學(xué)報(bào);2016年03期

2 蘇曉華;龐戰(zhàn)軍;;葡萄胎病理相關(guān)新基因F10與絨癌細(xì)胞系JAR成瘤性的關(guān)系研究[J];解放軍醫(yī)學(xué)雜志;2016年01期

3 蘇曉華;龐戰(zhàn)軍;蘇桂棟;;葡萄胎病理相關(guān)新基因F10與絨癌細(xì)胞系JEG-3成瘤性的關(guān)系[J];南方醫(yī)科大學(xué)學(xué)報(bào);2015年05期

4 蘇曉華;龐戰(zhàn)軍;;F10基因過(guò)表達(dá)及沉默對(duì)絨癌細(xì)胞系JAR侵襲相關(guān)蛋白酶表達(dá)的影響[J];醫(yī)學(xué)研究生學(xué)報(bào);2015年04期

5 龐戰(zhàn)軍;周君桂;;葡萄胎病理相關(guān)基因F10編碼蛋白單克隆抗體的制備與應(yīng)用[J];免疫學(xué)雜志;2015年02期

6 賀紅娟;陳爾飛;雷蕾;;腫瘤發(fā)生與相關(guān)基因突變的關(guān)聯(lián)[J];基因組學(xué)與應(yīng)用生物學(xué);2015年01期

7 付欣;李熹，

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