本文關(guān)鍵詞： 多囊卵巢綜合征 胰島素抵抗 來曲唑 高脂飲食　出處：《首都醫(yī)科大學(xué)學(xué)報(bào)》2017年02期 　論文類型：期刊論文

【摘要】：目的采用兩種不同喂養(yǎng)時(shí)間的來曲唑聯(lián)合高脂飼料方法,建立多囊卵巢綜合征胰島素抵抗(polycystic ovary syndrome with insulin resistance,PCOS-IR)大鼠模型,分析兩種模型各自的內(nèi)分泌和代謝特征。方法將18只6周齡SD雌性大鼠采用數(shù)字表法隨機(jī)分為3組:對照組、來曲唑A組和來曲唑B組,每組各6只。對照組予普通飼料喂養(yǎng),每日灌胃0.5%(質(zhì)量分?jǐn)?shù))羧甲纖維素鈉溶液;來曲唑A組連續(xù)21 d灌胃來曲唑溶液,同時(shí)給予高脂飼料喂養(yǎng);來曲唑B組連續(xù)30 d灌胃來曲唑溶液,并給予與來曲唑A組相同的高脂飼料喂養(yǎng)。觀察3組大鼠的體質(zhì)量和卵巢組織學(xué)變化,測定血清卵泡刺激素(follicle stimulating hormone,FSH)、黃體生成素(luteinizing hormone,LH)、睪酮(testosterone,T)、三酰甘油(triglyceride,TG)和空腹胰島素(fasting insulin,FINS)等濃度,計(jì)算LH/FSH比值和胰島素抵抗指數(shù)(homeostasis model of assessment for insulin resistence index,HOMAIR)。結(jié)果與對照組比較,來曲唑A組大鼠體質(zhì)量明顯增加,差異有統(tǒng)計(jì)學(xué)意義(P0.05),性激素及代謝等指標(biāo)較對照組有升高趨勢,但差異無統(tǒng)計(jì)學(xué)意義(P0.05)。來曲唑B組大鼠的體質(zhì)量亦有明顯增加(P0.05),卵巢呈多囊樣改變,血清T、LH/FSH顯著升高(P0.01),同時(shí),三酰甘油、空腹血糖(fasting plasma glucose,FPG)、空腹胰島素和HOMA-IR值均顯著升高(P0.01)。結(jié)論來曲唑聯(lián)合高脂飼料喂養(yǎng)30 d可成功誘導(dǎo)出多囊卵巢綜合征胰島素抵抗大鼠模型;與同法喂養(yǎng)21 d的模型相比,其內(nèi)分泌與代謝指標(biāo)改變更符合人PCOS-IR的臨床病理特征。
[Abstract]:Objective to adopt two different feeding methods of letrozole combined with high fat diet. To establish polycystic ovary syndrome with insulin resistance with polycystic ovary syndrome (PCOS). The endocrine and metabolic characteristics of the two models were analyzed. Methods 18 6-week-old SD female rats were randomly divided into three groups: control group. Letrozole A group and letrozole B group, 6 rats in each group. The control group was fed with normal diet and was given 0.5 (mass fraction) sodium carboxymethylcellulose solution daily. Letrozole A group was fed with high fat diet for 21 days. Letrozole B group was fed with letrozole solution for 30 days and fed with the same high-fat diet as letrozole A group. The changes of body weight and ovarian histology of rats in three groups were observed. Serum follicle stimulating hormone (follicle stimulating hormoneation) and luteinizing hormone were measured. LHG, testosterone testosterone, triglyceride triglyceride (TGG) and fasting insulin fasting insulin. Isoconcentration of fins. Calculate the LH/FSH ratio and insulin resistance index. Homeostasis model of assessment for insulin resistence index. Results compared with the control group, the body mass of Letrazole A rats was significantly increased, the difference was statistically significant (P 0.05), sex hormone and metabolism were higher than those in the control group. But the difference was not statistically significant (P 0.05). The body weight of rats in Letrozol B group also increased significantly (P 0.05), the ovary showed polycystic changes, serum T. LH/FSH significantly increased P0.01g, and triglyceride, fasting plasma glucose (FPGs). Fasting insulin and HOMA-IR increased significantly (P 0.01). Conclusion Letrazole combined with high fat diet for 30 days can successfully induce insulin resistance model of polycystic ovary syndrome in rats. Compared with the model fed with the same method for 21 days, the changes of endocrine and metabolism indexes were more consistent with the clinicopathological characteristics of human PCOS-IR.
【作者單位】： 首都醫(yī)科大學(xué)附屬北京中醫(yī)醫(yī)院婦科;
【基金】：國家自然科學(xué)基金(81674010)~~
【分類號】：R711.75;R-332
【正文快照】： 多囊卵巢綜合征(polycystic ovary syndrome,P-COS)是婦科常見的內(nèi)分泌與代謝功能異常的復(fù)雜性疾病,以持續(xù)無排卵或稀發(fā)排卵、高雄激素和/或高胰島素血癥、胰島素抵抗(insulin resistance,IR)為特征,表現(xiàn)為月經(jīng)稀發(fā)、閉經(jīng)、不孕、肥胖、多毛、痤瘡等[1-2]。據(jù)統(tǒng)計(jì),國外育齡期

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