本文關(guān)鍵詞： 子宮內(nèi)膜癌 SNCG 激光掃描共聚焦顯微鏡 細(xì)胞周期　出處：《實用醫(yī)學(xué)雜志》2017年22期 　論文類型：期刊論文

【摘要】：目的觀察γ-神經(jīng)突觸核蛋白(SNCG)基因穩(wěn)定沉默后人子宮內(nèi)膜癌HEC-1A細(xì)胞生長周期的變化。方法采用流式細(xì)胞術(shù)檢測SNCG基因沉默后HEC-1A細(xì)胞周期變化;通過激光掃描共聚焦顯微鏡觀察吖啶橙染色后G2/M期百分比變化。結(jié)果流式細(xì)胞術(shù)結(jié)果顯示,SNCG基因沉默后HEC-1A細(xì)胞處于G_1和G_2/M期增多,處于S期減少,差異有統(tǒng)計學(xué)意義(P0.05);共聚焦顯微鏡觀察結(jié)果顯示,SNCG基因沉默后HEC-1A細(xì)胞處于G_2/M期百分比增高,差異有統(tǒng)計學(xué)意義(P0.05)。結(jié)論兩個方法共同驗證了慢病毒質(zhì)粒載體shRNA干擾后SNCG基因穩(wěn)定沉默的HEC-1A細(xì)胞周期阻滯于G_2/M期,提示SNCG基因與子宮內(nèi)膜癌細(xì)胞生長周期密切相關(guān)。
[Abstract]:Objective to observe the expression of 緯 -neurosynaptophysin (SNCGG). Methods the changes of HEC-1A cell cycle after SNCG gene silencing were detected by flow cytometry. The percentage change of G _ 2 / M phase after acridine orange staining was observed by laser scanning confocal microscope. After SNCG gene silencing, the number of HEC-1A cells increased in Gsta1 and G2 / M phase, and decreased in S phase. The difference was statistically significant (P 0.05). The results of confocal microscopy showed that the percentage of HEC-1A cells in 2 / M phase was increased after the silencing of SNCG gene. The difference was statistically significant (P0.05). Conclusion the two methods combined to verify that the stable silencing HEC-1A cell cycle of SNCG gene after the interference of lentivirus plasmid shRNA was blocked in G _ S _ 2 / M phase. The results suggest that SNCG gene is closely related to the growth cycle of endometrial cancer cells.
【作者單位】： 廣西醫(yī)科大學(xué)第一附屬醫(yī)院婦科;廣東省深圳市光明新區(qū)人民醫(yī)院婦科;
【基金】：國家自然科學(xué)基金資助項目(編號:81460396)
【分類號】：R737.33
【正文快照】： γ-神經(jīng)突觸核蛋白(γ-synuclein,SNCG)是神經(jīng)突觸核蛋白家族的一員,一種致癌基因,最早發(fā)現(xiàn)于人乳腺癌c DNA文庫,又稱乳腺癌易感基因1(breast cancer susceptibility gene 1,BRCA1)。近年來,在乳腺癌、食管癌和膀胱癌等惡性腫瘤中相繼發(fā)現(xiàn)SNCG基因異常高表達(dá),且有大量研究報道，

本文編號：1461173