宮頸錐形切除術(shù)后宮頸組織中TNF-α、IL-6、HMGB1的表達(dá)及其意義研究
本文關(guān)鍵詞: 子宮頸錐形切除術(shù)后 子宮切除術(shù) 腫瘤壞死因子α 白細(xì)胞介素 高遷移率族蛋白質(zhì)類 術(shù)后感染 出處:《重慶醫(yī)學(xué)》2017年11期 論文類型:期刊論文
【摘要】:目的探討宮頸錐形切除術(shù)后宮頸分泌物及宮頸組織中炎性因子的動態(tài)變化及其意義。方法選取2013-2015年在該院就診的行宮頸錐形切除術(shù)患者,術(shù)后定期采集宮頸創(chuàng)面分泌物及周圍組織,定量檢測腫瘤壞死因子-α(TNF-α)、白細(xì)胞介素-6(IL-6)、高遷移率族蛋白1(HMGB1)的表達(dá),以術(shù)前作為對照組,進(jìn)行統(tǒng)計分析。觀察宮頸錐形切除術(shù)后行全子宮切除的標(biāo)本中炎性細(xì)胞的浸潤及表達(dá)。結(jié)果宮頸組織及分泌物中TNF-α、IL-6、HMGB1的表達(dá)在宮頸錐形切除術(shù)后逐漸升高,術(shù)后1~2周達(dá)到高峰,與術(shù)前比較差異有統(tǒng)計學(xué)意義(P0.05),之后逐漸下降。在宮頸錐形切除術(shù)后1周行全子宮切除的標(biāo)本病理顯示,炎性細(xì)胞浸潤及炎癥反應(yīng)最嚴(yán)重;其TNF-α、IL-6及HMGB1表達(dá)較術(shù)后4周明顯升高。結(jié)論宮頸錐形切除術(shù)后1~2周時宮頸創(chuàng)面炎癥最為嚴(yán)重,應(yīng)避免在此階段行全子宮切除。
[Abstract]:Objective to investigate the dynamic changes and significance of inflammatory factors in cervical secretions and cervical tissues after cervical conical resection. The secretion of cervical wound and its surrounding tissues were collected regularly after operation, and tumor necrosis factor- 偽 (TNF- 偽) and interleukin-6 (IL-6) were detected quantitatively. The expression of high mobility group protein 1 (HMGB1) was preoperatively used as the control group. To observe the infiltration and expression of inflammatory cells in the specimens after total hysterectomy. Results TNF- 偽 IL-6 was found in cervical tissues and secretions. The expression of HMGB1 increased gradually after cervical conical resection and reached its peak at 1 and 2 weeks postoperatively (P 0.05). The pathological results showed that inflammatory cell infiltration and inflammatory reaction were the most serious in the specimens with total hysterectomy 1 week after cervical conical resection. The expression of TNF- 偽 IL-6 and HMGB1 was significantly higher than that at 4 weeks after operation. Conclusion the inflammation of cervix wound is the most serious at 1 ~ 2 weeks after conical resection of cervix, and total hysterectomy should be avoided at this stage.
【作者單位】: 江蘇大學(xué)第四附屬醫(yī)院暨鎮(zhèn)江市婦幼保健院婦科;
【基金】:國家自然科學(xué)基金項(xiàng)目(81402165,81672913) 江蘇省自然科學(xué)基金項(xiàng)目(BK20141288) 江蘇省鎮(zhèn)江市科技支撐計劃-社會發(fā)展基金資助項(xiàng)目(SH2013088)
【分類號】:R737.33
【正文快照】: 在宮頸上皮內(nèi)瘤變Ⅲ級和子宮頸浸潤癌Ⅰa1期患者的處理過程中,宮頸錐形切除術(shù)是其診斷和治療的重要環(huán)節(jié)。接受宮頸錐形切除術(shù)的部分患者中后續(xù)需行全子宮切除術(shù)。本文連續(xù)檢測宮頸錐形切除術(shù)后宮頸組織及分泌物中腫瘤壞死因子-α(tumornecrosis factor-a,TNF-α)、白細(xì)胞介素-
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