本文關(guān)鍵詞：AQP9和Caspase7在羊水過少產(chǎn)婦胎盤及胎膜組織中的表達(dá)及其相關(guān)性　出處：《延安大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 羊水過少 胎盤 胎膜 水通道蛋白 凋亡蛋白

【摘要】：目的:通過檢測(cè)水通道蛋白9（AQP9）和凋亡蛋白Caspase7在正常羊水量孕產(chǎn)婦和羊水過少孕產(chǎn)婦胎盤及胎膜中的水平，探討AQP9和Caspase7與羊水過少的關(guān)系及其二者的相關(guān)性。 方法:選擇2013年6月至2013年11月在延安大學(xué)附屬醫(yī)院產(chǎn)科足月孕（37-41周）剖宮產(chǎn)分娩的羊水過少組孕產(chǎn)婦及正常組孕產(chǎn)婦各30例，兩組孕產(chǎn)婦年齡、孕周、孕次等均無顯著性差異，既往均無內(nèi)外科合并癥及其他妊娠合并癥（如妊娠期高血壓疾病、胎兒宮內(nèi)生長(zhǎng)受限、胎膜早破、胎兒畸形等），近期無藥物服用史、感染史及母體脫水等因素。采用免疫組化法(SP法)分別檢測(cè)30例正常羊水量和30例羊水過少孕產(chǎn)婦胎盤及胎膜組織中AQP9和Caspase7表達(dá)水平，對(duì)兩種蛋白的表達(dá)強(qiáng)度行半定量分析，并確定其表達(dá)部位。 結(jié)果： 1. Caspase7、AQP9在兩組孕產(chǎn)婦胎盤組織中的表達(dá) Caspase7、AQP9蛋白在兩組產(chǎn)婦的胎盤的細(xì)胞中均有陽性表達(dá)，，呈棕黃色，其陽性表達(dá)部位主要分布于胎盤合體滋養(yǎng)細(xì)胞的細(xì)胞膜和胞漿中。羊水過少組孕產(chǎn)婦胎盤滋養(yǎng)細(xì)胞中Caspase7的表達(dá)水平高于對(duì)照組(t=2.85),而AQP9蛋白的表達(dá)水平卻低于對(duì)照組(t=2.32)，差異均有統(tǒng)計(jì)學(xué)意義(P＜0.05). 2. Caspase7、AQP9在兩組孕產(chǎn)婦胎膜組織中的表達(dá) Caspase7、AQP9蛋白在兩組產(chǎn)婦的胎膜組織的細(xì)胞中均有陽性表達(dá)，呈棕黃色，其陽性表達(dá)部位主要分布于胎膜上皮細(xì)胞的細(xì)胞膜和胞漿中。羊水過少組孕產(chǎn)婦胎膜細(xì)胞中Caspase7的表達(dá)水平高于對(duì)照組(t=3.12),而AQP9蛋白的表達(dá)水平卻低于對(duì)照組(t=4.16)，差異均有統(tǒng)計(jì)學(xué)意義(P＜0.05)。 3. Caspase7、AQP9在兩組孕產(chǎn)婦胎盤胎膜組織中的表達(dá)關(guān)系 經(jīng)Spearman等級(jí)相關(guān)分析后發(fā)現(xiàn):正常組孕產(chǎn)婦胎盤滋養(yǎng)細(xì)胞及胎膜上皮細(xì)胞Caspase7與AQP9的表達(dá)系數(shù)分別為rs=0.12，rs=0.23，P均大于0.05，差異均無統(tǒng)計(jì)學(xué)意義;而在羊水過少組其值分別為rs= 0.63, rs= 0.78，P值均小于0.05，差異均有統(tǒng)計(jì)學(xué)意義。 結(jié)論： 1.兩組孕產(chǎn)婦胎盤胎膜中Caspase7蛋白與AQP9蛋白的表達(dá)差異均有統(tǒng)計(jì)學(xué)差異。因此羊水過少孕婦胎盤胎膜中Caspase7蛋白的高表達(dá)與AQP9蛋白的低表達(dá)，提示AQP9和Caspase7在產(chǎn)婦胎盤及胎膜中的異常表達(dá)與羊水過少的發(fā)生有密切關(guān)系。 2.正常組孕產(chǎn)婦胎盤胎膜細(xì)胞中Caspase7與AQP9的表達(dá)均無相關(guān)性關(guān)系，而在羊水過少組胎盤胎膜細(xì)胞中Caspase7與AQP9的表達(dá)水平均呈負(fù)相關(guān)。由此我們推測(cè)在羊水過少組的孕婦胎盤胎膜細(xì)胞上Caspase7的表達(dá)增加可誘導(dǎo)細(xì)胞自身凋亡增加,使得胎盤胎膜上AQP9的表達(dá)下降，影響胎盤胎膜上AQP9的結(jié)構(gòu)甚至功能,導(dǎo)致物質(zhì)交換障礙,進(jìn)一步影響體內(nèi)的水代謝及其循環(huán),從而降低羊水等物質(zhì)在母體、胎兒及胎膜之間的交換,導(dǎo)致羊水過少。
[Abstract]:Objective: to detect the levels of aquaporin 9 (AQP9) and apoptotic protein Caspase7 in placenta and membranes of normal amniotic fluid pregnant women and oligohydramnios women. To investigate the relationship between AQP9 and Caspase7 and oligohydramnios. Methods: 30 pregnant women with oligohydramnios and 30 normal pregnant women were selected from June 2013 to November 2013. There was no significant difference between the two groups in age, gestational week and second trimester. There were no complications of internal and surgical diseases and other pregnancy complications (such as hypertensive disorder complicating pregnancy, fetal growth restriction, premature rupture of membranes). Fetal malformation, no recent history of drug use. History of infection and maternal dehydration. Immunohistochemical method (SP method). The expression of AQP9 and Caspase7 in placenta and membranes of 30 cases of normal amniotic fluid and 30 cases of oligohydramnios were detected respectively. The expression intensity of the two proteins was determined by semi-quantitative analysis. Results: 1. Expression of Caspase7 and AQP9 in placenta of pregnant and parturient women The expression of Caspase7 and AQP9 protein in placental cells of both groups was brown and yellow. The expression of Caspase7 in placental trophoblastic cells in oligohydramnios group was higher than that in control group (2.85). . The expression of AQP9 protein was significantly lower than that of control group (P < 0.05). 2. Expression of Caspase7 and AQP9 in fetal membrane of pregnant and parturient women The expression of Caspase7 and AQP9 protein was brown in the cells of fetal membrane tissue of the two groups. The expression of Caspase7 in fetal membranes of oligohydramnios group was higher than that of control group (3.12). The expression of AQP9 protein was significantly lower than that of control group (P < 0.05). 3. Expression of Caspase7 and AQP9 in placental membranes of pregnant and parturient women Spearman grade correlation analysis showed that the expression coefficients of Caspase7 and AQP9 in placental trophoblastic cells and fetal membrane epithelial cells of normal pregnant women were rs=0.12 respectively. There was no significant difference between 0.23 P and 0.05; In oligohydramnios group, the values were 0.63, 0.78, P < 0.05, respectively. The difference was statistically significant. Conclusion: 1. The expression of Caspase7 protein and AQP9 protein in placental membranes of pregnant women with oligohydramnios was significantly different between the two groups. Therefore, the high expression of Caspase7 protein in placental membranes of oligohydramnios pregnant women. And the low expression of AQP9 protein. The results suggest that the abnormal expression of AQP9 and Caspase7 in placenta and membranes is closely related to the occurrence of oligohydramnios. 2. There was no correlation between the expression of Caspase7 and AQP9 in placental membranes of normal pregnant women. However, the expression of Caspase7 and AQP9 in placental membranes of oligohydramnios was negatively correlated with that in oligohydramnios. Therefore, we speculate that Caspase7 is expressed on placental membranes of pregnant women with oligohydramnios. The increase of cell apoptosis can be induced by the increase of cell apoptosis. The expression of AQP9 on placental membranes is decreased, the structure and even function of AQP9 on placental membranes are affected, the substance exchange is obstructed, and the water metabolism and its circulation are further affected. Thus reducing the exchange of amniotic fluid and other substances between the maternal, fetal and fetal membranes, resulting in oligohydramnios.
【學(xué)位授予單位】：延安大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R714.2

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