本文關(guān)鍵詞：microRNA-145的表達(dá)抑制子宮頸癌細(xì)胞的侵襲和遷移　出處：《吉林大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

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【摘要】：目的： 觀察microRNA-145的表達(dá)對(duì)子宮頸癌細(xì)胞侵襲和遷移能力的影響。 方法： 將實(shí)驗(yàn)室連續(xù)傳代培養(yǎng)的Hela細(xì)胞分為2組：（1）：轉(zhuǎn)染microRNA-145類似物的實(shí)驗(yàn)（M）組；（2）轉(zhuǎn)染miR-145模擬物陰性對(duì)照的對(duì)照（NC）組。 腫瘤細(xì)胞侵襲試驗(yàn)：將兩組細(xì)胞加入含有0.1%的FBS的DMEM培養(yǎng)基中饑餓18小時(shí)，然后將我們將細(xì)胞種在transwell小室上室內(nèi)，聚碳酸酯膜具有通透性，上室內(nèi)的細(xì)胞可以受下層培養(yǎng)液中的成分的影響，從而研究下層培養(yǎng)液的成分對(duì)細(xì)胞生長(zhǎng)、運(yùn)動(dòng)等的影響。用棉簽擦去基質(zhì)膠和上室內(nèi)的細(xì)胞后用結(jié)晶紫染色。隨機(jī)選取視野，觀察和拍照，，計(jì)算通過(guò)聚碳酸酯膜的細(xì)胞數(shù)。 腫瘤遷移實(shí)驗(yàn)：在實(shí)驗(yàn)組和對(duì)照組細(xì)胞生長(zhǎng)的中央?yún)^(qū)域用微量槍頭劃線，細(xì)胞的中央部分被去除，然后將無(wú)血清培養(yǎng)基加入其中，再放入37度5%CO2培養(yǎng)箱，繼續(xù)培養(yǎng)。至實(shí)驗(yàn)設(shè)定的時(shí)間（0，6，12，24小時(shí)），取出細(xì)胞培養(yǎng)板，觀察中央劃痕區(qū)是否有周邊細(xì)胞生長(zhǎng)，以此判斷細(xì)胞的遷移能力，按0，6，12，24小時(shí)取樣，拍照。 所得數(shù)據(jù)采用Image-Pro Plus6.0及SPSS15.0軟件作統(tǒng)計(jì)學(xué)處理和分析，比較兩組間差異程度是否具有統(tǒng)計(jì)學(xué)意義。 實(shí)驗(yàn)結(jié)果： 轉(zhuǎn)染microRNA-145類似物的實(shí)驗(yàn)組的侵襲和遷移能力顯著低于miR-145模擬物陰性對(duì)照的對(duì)照組。miR-145可通過(guò)調(diào)控一些特定的基因和蛋白來(lái)影響腫瘤細(xì)胞的侵襲和遷移。
[Abstract]:Objective: To observe the effect of microRNA-145 expression on invasion and migration of cervical cancer cells. Methods: The cultured Hela cells were divided into two groups: group 1: microRNA-145 analogue transfection group. 2) the control group transfected with miR-145 analogue negative control group. Tumor cell invasion test: two groups of cells were fed in DMEM medium containing 0.1% FBS for 18 hours, then we planted the cells in the transwell chamber. The polycarbonate membrane is permeable and the cells in the upper chamber can be affected by the components in the lower culture medium, so as to study the cell growth of the lower culture medium. The effect of exercise. The cells were stained with crystal violet with cotton swabs. The number of cells passing through the polycarbonate membrane was calculated by randomly selecting the field of vision, observing and taking pictures, and calculating the number of cells passing through the polycarbonate film. Tumor migration experiment: in the experimental group and control group, the central region of the cell growth was drawn with a trace shot head, the central part of the cell was removed, and then the serum-free medium was added into it. Then put it in 37 擄5 CO _ 2 incubator and continue culture. At the time of experiment, we took out the cell culture plate and observed whether there were peripheral cells growing in the central scratch area. To judge the migration ability of the cells, take samples for 24 hours and take pictures. The data were analyzed by Image-Pro Plus6.0 and SPSS15.0 software. Experimental results: The invasion and migration ability of the experimental group transfected with microRNA-145 analogues was significantly lower than that of the control group with negative miR-145 analog. Genes and proteins affect the invasion and migration of tumor cells.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.33

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 鐘麗;安云婷;;miR-145與婦科惡性腫瘤關(guān)系的研究進(jìn)展[J];實(shí)用臨床醫(yī)學(xué);2013年05期

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