本文關(guān)鍵詞：RNA干擾EFEMP1對(duì)卵巢癌細(xì)胞增殖、侵襲與轉(zhuǎn)移的影響　出處：《現(xiàn)代婦產(chǎn)科進(jìn)展》2016年01期 　論文類型：期刊論文

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【摘要】：目的:探討fibulin糖蛋白家族中fibulin-3(EFEMP1)對(duì)卵巢癌細(xì)胞增殖、侵襲與轉(zhuǎn)移的影響,為卵巢癌針對(duì)性治療提供理論基礎(chǔ)。方法:利用慢病毒轉(zhuǎn)染RNA干擾的方式,轉(zhuǎn)染具有高侵襲轉(zhuǎn)移能力的S1克隆細(xì)胞株,抑制EFEMP1表達(dá)。Western blot、Real-time RT-PCR和免疫組化對(duì)轉(zhuǎn)染效果進(jìn)行驗(yàn)證。采用體外細(xì)胞功能分析技術(shù)檢測(cè)干擾前后EFEMP1對(duì)細(xì)胞增殖、侵襲與轉(zhuǎn)移能力的影響。建立裸鼠皮下移植瘤模型,體內(nèi)觀察EFEMP1對(duì)腫瘤生長(zhǎng)的影響。結(jié)果:RNA干擾有效抑制EFEMP1表達(dá),EFEMP1降表達(dá)顯著抑制腫瘤細(xì)胞增殖、侵襲和轉(zhuǎn)移能力,裸鼠體內(nèi)成瘤率降低并且腫瘤體積顯著下降,同時(shí)腫瘤組織Fibulin基因表達(dá)顯著降低。結(jié)論:EFEMP1與卵巢癌發(fā)生發(fā)展及侵襲轉(zhuǎn)移密切相關(guān),為卵巢癌的治療提供新的思路。
[Abstract]:Objective: to investigate the effect of fibulin-3 (EFEMP1) in fibulin glycoprotein family on the proliferation, invasion and metastasis of ovarian cancer cells. Methods: using lentivirus transfection of RNA interference to transfect S1 clone cell line with high invasion and metastasis ability. Inhibition of EFEMP1 expression. Western blot. Real-time RT-PCR and immunohistochemistry were used to verify the transfection effect. In vitro cell function analysis was used to detect the proliferation of EFEMP1 before and after interference. The effect of EFEMP1 on tumor growth was observed in vivo. Results EFEMP1 interference could effectively inhibit the expression of EFEMP1. The down-regulated expression of EFEMP1 significantly inhibited the proliferation, invasion and metastasis of tumor cells, and the tumorigenesis rate and tumor volume in nude mice decreased significantly. At the same time, the expression of Fibulin gene in tumor tissue was significantly decreased. Conclusion the expression of Fibulin gene is closely related to the occurrence, development, invasion and metastasis of ovarian cancer, which provides a new idea for the treatment of ovarian cancer.
【作者單位】： 山東大學(xué)公共衛(wèi)生學(xué)院少兒衛(wèi)生與婦幼保健系;
【基金】：國(guó)家自然基金青年基金(NO:81202056)
【分類號(hào)】：R737.31
【正文快照】： 卵巢腫瘤為婦科生殖系統(tǒng)常見(jiàn)腫瘤,死亡率居?jì)D科惡性腫瘤首位,嚴(yán)重危害婦女的身心健康[1-2]。由于缺乏有效的早期診斷和治療方法,大部分患者確診時(shí)已為晚期,其5年生存率為25%~30%[3]。 近年卵巢癌治療已取得重大進(jìn)展,但其死亡率仍高居不下。深入研究發(fā)現(xiàn),腫瘤的浸潤(rùn)與轉(zhuǎn)移構(gòu)成，

本文編號(hào)：1390412