目的：研究促紅細(xì)胞生成素與黃芪對新生兒窒息后血清誘導(dǎo)人腎小管細(xì)胞TLR4/MyD88 （Toll-like receptors 4/myeloid differentiation factor 88）表達(dá)的影響方法：（1）以HK-2細(xì)胞（Human renal proximal tubular cell,人近曲腎小管上皮細(xì)胞）作為研究對象。（2）以足月重度窒息（生后Apgar評分均小于4分）新生兒第一個(gè)24小時(shí)血清（20%體積分?jǐn)?shù)）作為致病因素。（3）實(shí)驗(yàn)分四組,即對照組、窒息組、EPO（erythropoietin,促紅細(xì)胞生成素）組、黃芪（Astragalus, AS）組。（4）檢測指標(biāo)：倒置相差顯微鏡下觀察HK-2細(xì)胞形態(tài)變化；流式細(xì)胞儀（Flow Cytometry, FCM）檢測各組細(xì)胞TLR4特異性熒光抗體陽性結(jié)合率表達(dá)情況；采用免疫組織化學(xué)法（SP法）檢測各組細(xì)胞MyD88表達(dá)情況。（5）統(tǒng)計(jì)學(xué)分析：數(shù)據(jù)均用x±s表示,采用one-way ANOVA分析,組間比較用LSD法,運(yùn)用SPSS14.0軟件進(jìn)行統(tǒng)計(jì)學(xué)處理,P<0.05為差異具有統(tǒng)計(jì)學(xué)意義。結(jié)果：（1）倒置相... 

【文章來源】：西南醫(yī)科大學(xué)四川省

【文章頁數(shù)】：65 頁

【學(xué)位級別】：碩士

【部分圖文】：

EPO和黃蔑對新生兒窒息后血清誘導(dǎo)人CDHK--2細(xì)胞形態(tài)學(xué)變化的影響(光

附圖圖1EPO和黃蔑對新生兒窒息后血清誘導(dǎo)人鏡X400)。CDHK--2細(xì)胞形態(tài)學(xué)變化的影響(光A:對照組B:窒息組C:EPO組D:黃蔑組Figure1EffeetofErythroPoietinandAstr昭a(bǔ)lusontheehangesofmorPhology(IH，underC:groupinH恤anHK--2indueedbyPostasPhyxial一ser恤ofneonateslightmieroseoPyX400).A:eontrolgrouP，B:asPhyxiagrouP，Astragalus.PretreatlllentwithEPO，D:grouPofPretreatment賈ith

Astragalus.一一。八八八.八八.../反反反反反反反反反一一一一洲火火，‘’耐認(rèn)’志圖2EPO和黃蔑對新生兒窒息后血清誘導(dǎo)人HKesZ細(xì)胞TL凡特異性熒光抗體陽性結(jié)合率表達(dá)的影響A:對照組B:窒息組c:EPO組D:黃蔑組Figure2EffeetsofErythroPoietinandAstragalusontheexPressionofthePositivebindingrateofsPeeialfluoreseentantibodyinTLR;onHKesZeellindueedbypostasphyxial一serumofneonates(F以).A:eontrolgroup，B:asPhyxiagrouP，C:grouPofPretreat.entwithEPOD:grouPofPretreat.ent下ithAstragalus.

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